CRISPR/Cas12a-based biosensors for environmental monitoring and diagnostics
Environmental Technology & Innovation,
Journal Year:
2024,
Volume and Issue:
34, P. 103625 - 103625
Published: April 4, 2024
Contaminants,
such
as
nucleic
acids
or
toxic
small
molecules,
threaten
both
human
health
and
ecosystems
when
they
infiltrate
the
environment.
The
precise
highly
sensitive
identification
of
contaminants
holds
paramount
importance
across
diverse
domains,
including
safeguarding
food
integrity,
facilitating
clinical
diagnostics,
monitoring
environmental
conditions.
Traditional
methodologies,
encompassing
spectroscopy,
chromatography,
sequencing,
metagenomics,
have
conventionally
served
pivotal
roles
in
detection
processes.
Nevertheless,
these
methods
encountered
recurring
challenges
related
to
sensitivity,
specificity,
portability.
This
review
focuses
on
groundbreaking
CRISPR/Cas12-based
biosensors.
These
biosensors
leverage
incredible
precision
programmability
CRISPR/Cas
system
recognize
specific
targets.
Here,
we
comprehensively
assess
fundamental
mechanisms
that
enable
detection,
ranging
from
guide
RNA
design
collateral
cleavage.
versatility
CRISPR/Cas12
becomes
evident
through
their
applications.
applications
encompass
medical
safety,
monitoring.
transition
conventional
ultimately
represents
a
significant
milestone
contaminant
detection.
By
incorporating
molecular
biology,
nanotechnology,
bioinformatics,
potential
reshape
landscape
water
CRIPSR-Cas
diagnostics
is
transformative
technology
paves
way
for
safer
healthier
future
environment
life.
Language: Английский
Influence of Spacing on the Retention Process of Cascade Permeable Dams for Upstream Sediment-Laden Flow
Jian Liu,
No information about this author
Hong Zhou,
No information about this author
Longyang Pan
No information about this author
et al.
Water,
Journal Year:
2025,
Volume and Issue:
17(1), P. 95 - 95
Published: Jan. 1, 2025
Permeable
dams
are
an
important
means
for
river
management
and
ecology
protection.
Reasonable
dam
spacing
will
help
regulate
sediment
transport
reduce
load
in
lakes.
Flume
experiments
were
carried
out
to
investigate
the
effects
of
hydrological
conditions
on
retention
performance
permeability
cascade
permeable
dams.
The
experimental
results
show
that
coefficient
1#
decreased
by
about
30–40%
with
a
large
rate
during
initial
experiment
stage.
decrease
amplitude
rising
water
level
front
(D/L)
positively
correlated
flow
rate.
At
D/L
=
5,
difference
at
end
was
significantly
higher
than
other
spacing.
mass
retained
accounts
41–65%
total
retained,
which
is
twice
2#
dam,
plays
major
role
A
mathematical
model
predicting
spatial-temporal
concentration
inside
proposed
based
seepage
theory
porous
media.
research
great
guiding
significance
design
parameters.
Language: Английский
Water and Sediment Quantity and Quality Generated in Check Dams as a Nature-Based Solutions (NbS)
Water,
Journal Year:
2025,
Volume and Issue:
17(6), P. 810 - 810
Published: March 12, 2025
The
study
evaluates
the
implementation
of
check
dams
as
nature-based
solutions
to
address
soil
erosion,
improve
sediment
quality,
and
enhance
water
retention
in
Urku
Huayku
ravine,
located
on
Ilaló
volcano
Ecuador.
Weekly
samples
were
analysed
from
2021
2023.
Critical
parameters
measured
include
pH,
electrical
conductivity,
nutrient
concentrations,
organic
matter
content.
Macroinvertebrates
collected
assess
biodiversity
changes
using
Andean
Biotic
Index
(ABI)
Shannon
Diversity
Index.
Results
show
significant
improvements:
quality
remained
neutral
(average
pH
7.06),
while
increased
0.2%
3.2%
Additionally,
improved,
with
a
355.6%
increase
macroinvertebrate
abundance.
Statistical
tests
confirmed
positive
impact
quality.
also
identified
potential
areas
for
additional
dam
installations
QGIS
analysis,
emphasising
steep
slopes
ideal
locations.
This
demonstrates
efficacy
restoration
degraded
ecosystems
underscores
their
pivotal
role
climate
change
mitigation.
Through
enhanced
storage
matter,
facilitated
capture
approximately
58%
carbon.
they
contributed
improved
biodiversity.
Further
research
is
recommended
optimise
placement
explore
indicators
Andes
Mountain
bodies
over
3000
m
above
sea
level.
Language: Английский
Evaluation of adaptive capacity of slope regulation and storage measures on the Loess Plateau under drought stress
Siying Yan,
No information about this author
Baisha Weng,
No information about this author
Denghua Yan
No information about this author
et al.
Agricultural Water Management,
Journal Year:
2024,
Volume and Issue:
306, P. 109184 - 109184
Published: Nov. 20, 2024
Language: Английский
Development of in-situ anisotropic deformation characteristics detection equipment for loess hole
Measurement,
Journal Year:
2024,
Volume and Issue:
unknown, P. 116427 - 116427
Published: Dec. 1, 2024
Language: Английский
Problem of Improving the Environmental and Socio-Economic System of the Volga-Akhtuba Floodplain: Position Examination of Large Dam in the Volga River Based on Computational Fluid Dynamics
2022 4th International Conference on Control Systems, Mathematical Modeling, Automation and Energy Efficiency (SUMMA),
Journal Year:
2023,
Volume and Issue:
unknown, P. 752 - 756
Published: Nov. 8, 2023
The
system
of
hydroelectric
power
stations
on
the
Volga
River,
built
in
20th
century,
completely
changed
hydrological
regime
River.
current
over-regulation
water
flow
is
cause
accelerating
degradation
Volga-Akhtuba
floodplain.
Modern
analysis
availability
indicates
a
critical
point
associated
with
spring
filling
Akhtuba
binnacle.
Various
projects
suggest
solution
to
this
problem.
This
study
aims
at
identifying
most
optimal
location
large
dam
below
Volzhsky
stations.
We
use
new
model
hydraulic
resistance
based
combined
effect
both
friction
force
Manning
and
turbulent
viscosity.
Our
involves
varying
dam's
numerical
shallow
model.
calculate
relationship
between
release
through
source
Akhtuba,
as
target
function
for
analyzing
effectiveness
project
construction
such
dam.
Language: Английский