Enhanced capacity of a leaf beetle to combat dual stress from entomopathogens and herbicides mediated by associated microbiota

Integrative Zoology, Journal Year: 2024, Volume and Issue: 19(6), P. 1092 - 1104

Published: Feb. 20, 2024

Herbicides have demonstrated their impact on insect fitness by affecting associated microbiota or altering the virulence of entomopathogenic fungi toward insects. However, limited research has explored implications herbicide stress intricate tripartite interaction among insects, bacterial communities, and entomopathogens. In this study, we initially that bacteria confer a leaf beetle, Plagiodera versicolora, with capability to resist fungus Aspergillus nomius infection, sustained even under glyphosate stress. Further analysis revealed significant alteration in abundance composition due treatment. The dominant bacterium, post A. infection following combination treatments, exhibited strong suppressive effects fungal growth. Additionally, markedly inhibited pathogenic bacterium Pseudomonas though it P. versicolora's immunity, ultimately enhancing beetle's tolerance nomius. summary, our findings suggest bestow an augmented resilience against dual stressors both entomopathogen glyphosate. These results provide insight into residues interactions bacteria, fungi, holding for pest control ecosystem assessment.

Language: Английский

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Aquatic ecotoxicity of glyphosate, its formulations, and co-formulants: evidence from 2010 to 2023

Environmental Sciences Europe, Journal Year: 2024, Volume and Issue: 36(1)

Published: Feb. 6, 2024

Abstract Glyphosate (GLY), the most widely used herbicide active ingredient (AI) in world, is frequently detected aquatic environments where it can affect non-target organisms. Globally, more than 2000 commercial GLY-based herbicides (GBHs) are to control weeds. Non-target organisms exposed complex pesticide formulations under real environmental conditions, but co-formulants contained GBHs classified as so-called inert and inactive ingredients terms of their biological effects. The main objective this comprehensive review compile results ecotoxicological studies on side-effects GLY, GBHs, formulating agents. Based demonstrated for a variety plant animal organisms, oxidative stress appears be major trigger these adverse effects, affecting integrity DNA other biochemical functions. Furthermore, there evidence impairment various physiological behavioral Adverse effects GLY have been observed even at very low concentrations. There also differences sensitivity tested, with similar lifestyles, habitats or identical taxa. typically investigate short-term single exposure GLY/GBH species, whilst reality multiple applications together pesticides common during cropping cycle. Moreover, interactions between GLY/GBHs contaminants rarely studied. Higher toxicity compared alone has often observed, demonstrating that highly toxic own markedly increase GBH formulation. possible impurities such heavy metals, cause additional problems environment food safety. widespread massive use leads increased hazards. In addition, need revision risk assessment system emphasized. According studies, current pollution by problematic cannot considered environmentally sustainable. It is, therefore, necessary least tighten permitted forms use.

Language: Английский

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Elucidating the effects of pure glyphosate and a commercial formulation on early life stages of zebrafish using a complete biomarker approach: All-or-nothing!

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 916, P. 170012 - 170012

Published: Jan. 19, 2024

Language: Английский

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Glyphosate- and aminomethylphosphonic acid (AMPA)-induced mortality and residues in juvenile brown trout (Salmo trutta f. fario) exposed at different temperatures

Environmental Sciences Europe, Journal Year: 2024, Volume and Issue: 36(1)

Published: Feb. 16, 2024

Abstract Background Glyphosate is a broad-spectrum, non-selective systemic herbicide with commonly assumed low potential for accumulation in biota. Nevertheless, glyphosate has been shown to bioaccumulate the tissues of several organisms. To understand bioconcentration dynamics fish, brown trout ( Salmo trutta forma fario ) different age were exposed concentrations glyphosate, formulation Roundup® LB Plus, and major transformation product aminomethylphosphonic acid (AMPA) two, three, or four weeks at temperatures laboratory. Mortality rates determined, tissue samples collected end experiment ascertain AMPA residues by liquid chromatography coupled mass spectrometry (LC–MS/MS). Results Brown mortality during exposure was considerably higher 15 °C than 7 °C. Also, significant increase containing muscle, head, backbone, caudal fin increasing observed. Six-month-old fish contained more per kg wet weight after ten-month-old fish. The factors (BCFs) much °C, but both cases decreased concentrations. BCF formulated one parent compound. Approximately 30–42% organ-absorbed remained even when kept clean water lacking test substances three termination exposure. Conclusion Our study demonstrated that there an interaction between ambient temperature terms toxicity. Further it surrounding media lead significantly increased these tissues, although neither nor bioaccumulation animal expected due high solubility this chemical. As consequence, uptake humans through consumption contaminated edible very likely.

Language: Английский

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Overview of human health effects related to glyphosate exposure

Frontiers in Toxicology, Journal Year: 2024, Volume and Issue: 6

Published: Sept. 18, 2024

Glyphosate is a chemical compound derived from glycine, marketed as broad-spectrum herbicide, and represents one of the most widely used pesticides in world. For long time, it was assumed that glyphosate harmless, either due to its selective enzymatic acting method on plants, because commercial formulations were believed contain only inert chemicals. spread environment, general population daily exposed via different routes, including consumption both plant, non-plant based foods. has been detected high amounts workers’ urine, but likewise bodily fluids, such blood maternal milk, also 60%–80% population, children. Considering massive presence, exposure could be considered health risk for humans. Indeed, 2015, IARC (International Agency Research Cancer) classified derivatives Group 2A, probable human carcinogens. In 2022, nevertheless, EFSA (European Food Safety Authority) stated available data did not provide sufficient evidence prove mutagenic/carcinogenic effects glyphosate. Therefore, European Commission (EC) decided renew approval another 10 years. The purpose this review examine scientific literature, focusing potential risks arising glyphosate, metabolites products (e.g., Roundup ® ), with particular regard mutagenic carcinogenic endocrine disrupter (ED) especially reproductive system.

Language: Английский

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Investigating the genomic and biochemical effects of dalapon on antioxidant systems in zebrafish, Danio rerio

Toxicology Mechanisms and Methods, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 16

Published: March 12, 2025

This research explored the effects of dalapon exposure on expression various genes, including cat, sod1, sod2, sod3a, sod3b, gpx1a, gpx3, gpx4a, gpx4b, gpx7, gpx8, gpx9, gstr, g6pd, and gsr, along with activities related antioxidant enzymes (AEs), such as CAT, SOD, GPX, G6PD, GST, GR in zebrafish. Kidney liver tissues were analyzed to assess oxidative stress levels. Results indicated that both concentration (25 50 ppm) duration had a significant effect AE gene expression. RT-PCR analysis suggested changes among dalapon-exposed zebrafish might indicate rapid response pesticide-induced stress. Moreover, GST increased at specified concentrations. In contrast, prolonged exceeding 72 h led significantly higher malondialdehyde levels kidney compared control group. These findings enhance our understanding role provide important insights for developing aquaculture breeding programs focused improving fish tolerance. Furthermore, phylogenetic conserved synteny confirmed enzyme genes are orthologous those found other model organisms, medaka stickleback. Consequently, these results could be beneficial vertebrate species.

Language: Английский

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Comparative Aquatic Risk of Three Glyphosate–Based Herbicides Using Early‐Stage Development of Clarias gariepinus (Burchell, 1822)

Journal of Toxicology, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Clarias gariepinus’ (Burchell, 1822) early‐stage development was assessed in microconcentrations of glyphosate‐based herbicides (GBHs), Forceup, Roundup, and Uproot. Using the default ecological trigger value 0.37 mg L −1 glyphosate as a reference, were diluted to containing 0.006, 0.013, 0.025, 0.05, 0.10 ([v/v] %) herbicide using borehole water, which served control. Concentrations control replicated three times. Fertilization (%), time morula formation commencement hatching (minutes), (% fertilized eggs), 96‐h larval survival hatched larvae) monitored. Within formulation, concentration significantly affected fertilization rates ( p < 0.001), hatching, ([ χ 2 ] 5 = 16,648, 0.010; [Kruskal–Wallis H test]). Morula formation, fertilization, by formulation 3, while rate at all concentrations 3 6.49, 0.039). The well recommended application Roundup Proactive aquatic riparian environments 0.32% (v/v) are higher than lowest significant effect herbicides. Reactive oxygen species (ROS) superoxide dismutase (SOD) embryos, but not significantly, levels freshly stripped eggs > 0.05) (Mann–Whitney U test). Early‐stage normal controls, suggesting balance between ROS SOD. This was, however upset treatments, leading deleterious effects on development. GBHs pose greater risk fish reproduction, varying severity with formulation. should be considered regulations for their use environments, balancing effectiveness toxicity.

Language: Английский

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Environmental concentrations of glyphosate through direct or parental exposure alter nervous system development and reduce the fertility rate in zebrafish

NeuroToxicology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Effect of glyphosate, its metabolite AMPA, and the glyphosate formulation Roundup® on brown trout (Salmo trutta f. fario) gut microbiome diversity

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 15, 2024

Glyphosate is used worldwide as a compound of pesticides and detectable in many environmental compartments. It enters water bodies primarily through drift from agricultural areas so that aquatic organisms are exposed to this chemical, especially after rain events. advertised sold highly specific herbicide, which interacts with the EPSP synthase, an enzyme shikimate metabolism, resulting inhibition synthesis vital aromatic amino acids. However, not only plants but also bacteria can possess influences glyphosate on microbiomes cannot be excluded. Those may result subtle long-term effects, e.g., disturbance symbiotic interactions bionts microorganisms their microbiomes. Mechanisms how transformation product aminomethylphosphonic acid (AMPA) might interfere context have understood far. In present study, molecular biological fingerprinting methods showed concentration-dependent effects AMPA fish addition, age-dependent differences composition regarding abundance diversity were detected. Furthermore, effect exposure was investigated for several pathogens gut terms gene expression virulence factors associated pathogenicity. vitro transcriptome analysis pathogen Yersinia ruckeri revealed it questionable whether observed microbiome caused by intended mode action glyphosate, such synthase activity.

Language: Английский

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In vivo estrogenicity of glyphosate, its formulations, and AMPA on transgenic zebrafish (Danio rerio) embryos

Environmental Pollution, Journal Year: 2023, Volume and Issue: 342, P. 123113 - 123113

Published: Dec. 8, 2023

In this study, the disrupting effects of glyphosate (GLY), aminomethylphosphonic acid (AMPA), and three glyphosate-based herbicides (GBHs) on vitellogenesis in a non-concentration-dependent manner are reported for first time 120 h acute exposure zebrafish at environmentally relevant concentrations. GBHs commonly used worldwide weed control management. Due to their extensive application, they frequently occur aquatic ecosystems may affect various organisms. The active substance GLY its major by-product, AMPA, most thoroughly studied chemicals; however, adverse complex formulas with diverse unknown content co-formulants still not sufficiently researched. This study focused embryotoxicity, sublethal malformations, estrogenic potency GLY, four embryos using wild type an estrogen-sensitive, transgenic line (Tg(vtg1:mCherry)). After exposition, AMPA did cause toxicity, while LC

Language: Английский

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