Production of an amylase from newly Bacillus strain: Optimization by response-surface methodology, characterization and application with a fungal lipase in bread making DOI
Sameh Ben Mabrouk,

Bouthaina Ben Hadj Hmida,

Haïfa Sebii

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138147 - 138147

Published: Nov. 1, 2024

Language: Английский

Comparing a polynomial DOE model and an ANN model for enhanced geranyl cinnamate biosynthesis with Novozym® 435 lipase DOI
João Francisco Cabral do Nascimento, Bianca Dalbem dos Reis, Álvaro de Baptista Neto

et al.

Biocatalysis and Agricultural Biotechnology, Journal Year: 2024, Volume and Issue: 58, P. 103240 - 103240

Published: May 17, 2024

Language: Английский

Citations

6
Optimization of citrulline production from a Bacillus subtilis BH-01 isolated from raw buffalo milk DOI Creative Commons

Marwa A. K. Mansour,

Salah Ali,

Manal Hassan

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 10, 2025

Abstract The main purpose of this study was to optimize the L-citrulline production process using Plackett-Burman and Box-Behnken designs. L-citrulline-producing bacterium BH-01 isolated from raw buffalo milk. isolate tested for probiotic activities such as tolerance simulated gastric intestinal juices, antagonistic activity against six antibiotic-resistant bacteria, temperature tolerance. arginine deiminase (ADI) were optimized statistical bacterial molecularly identified Bacillus subtilis strain AUMC B-498 (accession number PP574248.1). exhibited resistance at pH 2.0 bile salt 0.5% a two-hour exposure period. It could inhibit growth Escherichia coli , Klebsiella pneumonia Serratia sp., Staphylococcus aureus methicillin - resistant (MRSA), Streptococcus pneumoniae. From results optimization, design temperature, L-arginine, incubation period, peptone most effective factors among eight selected variables. Based on these, used required maximize citrulline production. maximum 632.5 µg/L, ADI 1.42 U/mL. Therefore, Buffalo milk might be promising candidate in food, biotechnological, pharmaceutical applications due its dual functionality characteristics.

Language: Английский

Citations

0
Multi-criteria selectivity of PLA polymer 3D printing parameters: impact on the roughness of finished surfaces DOI

Mehrez Ben Rezg,

Mohamed Nasser,

Romdhane Othmani

et al.

Progress in Additive Manufacturing, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Language: Английский

Citations

0
Optimizing electrocoagulation parameters for enhanced leachate remediation DOI
Jamal Mabrouki, Karima Azoulay, Imane Bencheikh

et al.

Journal of Dispersion Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 17

Published: May 2, 2025

Language: Английский

Citations

0
Chitinase-functionalized UiO-66 framework nanoparticles active against multidrug-resistant Candida Auris DOI Creative Commons
Shaymaa A. Ismail, Bahgat Fayed, Reda M. Abdelhameed

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 20, 2024

Abstract Candida auris ( C. ) is a yeast that has caused several outbreaks in the last decade. Cell wall chitin plays primary role antifungal resistance of . Herein, we investigated potential chitinase immobilized with UiO-66 to act as potent agent against Chitinase was produced from Talaromyces varians SSW3 yield 8.97 U/g dry substrate (ds). The statistically enhanced 120.41 ds by using Plackett–Burman and Box–Behnken design. We synthesized framework characterized SEM, TEM, XRD, FTIR, particle size analyzer, zeta sizer. had 70.42 ± 8.43 nm uniform cubic shape smooth surface. on an immobilization 65% achieved after 6 h loading period. increased enzyme activity stability, indicated obtained K d T 1/2 values. Furthermore, hydrolytic UiO-66, increase V max decrease m 2- 38-fold, respectively. Interestingly, boosted MIC 50 0.89 0.056 U/mL, compared 5.582 0.57 U/mL for free enzyme. This study offers novel promising alternative approach combat new emerging pathogen auris.

Language: Английский

Citations

3
Application of natural fungi in bioconversion of lignocellulosic waste to second-generation ethanol DOI Creative Commons
Marina Fomina, Olga Yurieva, Artem Pavlychenko

et al.

Biosystems Diversity, Journal Year: 2024, Volume and Issue: 32(1), P. 45 - 59

Published: Jan. 7, 2024

The second generation (2G) or cellulosic ethanol can help with diversification of the use fossil energy sources. However, as bioconversion plant waste into 2G bioethanol requires expensive additional steps pre-treatment/hydrolysis lignocellulosic materials, and this technology has not yet reached technological readiness level which would allow it to be scaled-up, process needs more interdisciplinary comprehensive studies. This work was aimed at experimental study a full cycle successive processes pre-treatment/saccharification using cellulolytic enzymes filamentous fungi fermentation obtained syrups by xylose-fermenting yeast, selected natural microorganisms for fungal-based agricultural ethanol. Using Plackett-Burman Box-Behnken methods mathematical statistics, optimal conditions pre-treatment enzymatic hydrolysis wheat straw hemi- multi-enzyme complex fungal strain Talaromyces funiculosus UCM F-16795 were established: microwave-assisted alkali sodium hydroxide (NaOH) solutions (concentration range 4.6–4.8%), saccharification medium pH 4, temperature 40 °С, duration 18 hours, dilution culture liquid buffer solution 1:1. total microwave irradiation 1.2 kJ ratio substrate/enzyme 100 mg/1 mL used. Under optimized conditions, hydrolysates contained 5.0–7.5 g/L reducing sugars, which, according HPLC assessment, 0.7–1.0 glucose, 2.2–2.9 xylose 0.7–0.8 cellobiose. We used yeast in mixtures most important monosaccharides hydrolysates, concentration relevant us during substrates T. enzymes. Based on sequencing phylogenetic analysis, Y-2810 confirmed Scheffersomyces stipitis; its nucleotide sequences ITS region 28S gene rDNA deposited GenBank under accession numbers OP931914 OP931915, respectively. ethanologenic S. stipitis studied design, assessing gas chromatography-mass spectrometry. Yeast static microaerophilic showed 1.5 times higher rate production 1.7 greater efficiency ethanologenesis per biomass than submerged cultivation. Optimization resulted maximum mixture being 11.30 ± 0.36 ethanol, values factors: 30 xylose, 5.5 glucose cultivation days. It revealed that tested concentrations did significantly affect xylose-fermentation non-competitive inhibition transport cells occur. demonstrated potential based strains optimization all steps.

Language: Английский

Citations

2
Production of an amylase from newly Bacillus strain: Optimization by response-surface methodology, characterization and application with a fungal lipase in bread making DOI
Sameh Ben Mabrouk,

Bouthaina Ben Hadj Hmida,

Haïfa Sebii

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138147 - 138147

Published: Nov. 1, 2024

Language: Английский

Citations

0