An Engineered Yeast Expressing an Artificial Heavy Metal-Binding Protein Enhances the Phytoremediation of Alum Mine Soils DOI Creative Commons
Wenming Wang,

Liling Xie,

Lin Zhao

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 612 - 612

Published: March 7, 2025

Alum mining leads to significant heavy metal and acid pollution within soils. Phytoremediation is a common strategy used treat alum mine soils, but its efficiency frequently compromised by the alum-mining-induced impairment of plant growth. To improve strength plants against pollution, this study constructed artificial yeast strain ScHB (heavy metal-binding protein-containing Saccharomyces cerevisiae) expressing de novo designed protein HBGFP green fluorescence protein) investigated effect on phytoremediation soils with soil physiochemical assays quantification. This was composed an N-terminal signal peptide, HB metal-binding) domain, GFP (green as well C-terminal glycolphosphatidylinositol-anchoring fragment. The exposure surface increased growth rate cells enhanced cadmium capture from cadmium-containing medium. After culturing Medicago sativa in for 30 days, remarkably plants’ average height 17.5 cm 27.9 their biomass 3.03 g/plant 4.35 g/plant, increasing accumulation antioxidant agents plants. Moreover, strongly improved quality, increase pH values 5.47 6.21 6.9, levels organic matter, total nitrogen, available phosphorus, living bacteria. Furthermore, efficiently abilities remove metals, decreasing cadmium, lead, chromium, copper 90%, 86%, 97%, 88%, respectively. developed genetic engineering method mining.

Language: Английский

Controllable Preparation of Low-Cost Coal Gangue-Based SAPO-5 Molecular Sieve and Its Adsorption Performance for Heavy Metal Ions DOI Creative Commons
Le Kang,

Boyang Xu,

Pengfei Li

et al.

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(5), P. 366 - 366

Published: Feb. 27, 2025

With the advancement of industrial production and urban modernization, pollution from heavy metal ions accumulation solid waste have become critical global environmental challenges. Establishing an effective recycling system for removing metals wastewater is essential. Coal gangue was used in this study as primary material synthesis a fully coal gangue-based phosphorus-silicon-aluminum (SAPO-5) molecular sieve through hydrothermal process. The SAPO-5 characterized several methods, including X-ray diffraction (XRD), scanning electron microscopy (SEM), BET surface analysis, Fourier-transform infrared (FT-IR) spectroscopy, photoelectron spectroscopy (XPS), to examine its mineral phases, microstructure, pore characteristics, structure. Adsorption performance towards with Cd2+ Pb2+ investigated. It found that adsorption processes these are well described by both pseudo-second-order model Langmuir isotherm. According model, exhibited maximum capacities 93.63 mg·g-1 157.73 Pb2+. After five cycles, retained strong stability adsorbing Pb2+, residual 77.03 138.21 excellent mainly attributed mesoporous channel effects, complexation -OH functional groups, electrostatic attraction.

Language: Английский

Citations

2
Microbe-assisted phytoremediation for sustainable management of heavy metal in wastewater - A green approach to escalate the remediation of heavy metals DOI

Rashmi Ranjan Mandal,

Zahid Bashir,

Deep Raj

et al.

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 375, P. 124199 - 124199

Published: Jan. 22, 2025

Language: Английский

Citations

0
Evaluation of Metal Accumulation in Escherichia coli Expressing SPL2 by Single-Cell Inductively Coupled Plasma Mass Spectrometry DOI Open Access
Yasunori Fukumoto,

En-long LI,

Yuki Tanaka

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1905 - 1905

Published: Feb. 22, 2025

Rare earth elements, comprising 17 elements including 15 lanthanides, are essential components in numerous high-tech applications. While physicochemical methods commonly employed to remove toxic heavy metals (e.g., cadmium and mercury) from industrial wastewater, biological approaches offer increasingly attractive alternatives. Biomining, which utilizes microorganisms extract valuable ores wastes, bioremediation, leverages adsorb transport metal ions into cells via active transport, provide eco-friendly solutions for resource recovery environmental remediation. In this study, we investigated the potential of three recently identified lanthanide-binding proteins-SPL2, lanpepsy, lanmodulin-for applications these areas using single-cell inductively coupled plasma mass spectrometry (scICP-MS). Our results demonstrate that SPL2 exhibits superior characteristics lanthanide bioremediation. Heterologous expression a cytosolic fragment bacteria resulted high levels solubility. Single-cell ICP-MS analysis revealed recombinant accumulated lanthanum, cobalt, nickel, cadmium, effectively sequestering lanthanum culture media. Furthermore, conferred enhanced bacterial tolerance exposure. These findings establish as promising candidate developing systems bioremediation rare element biomining.

Language: Английский

Citations

0
An Engineered Yeast Expressing an Artificial Heavy Metal-Binding Protein Enhances the Phytoremediation of Alum Mine Soils DOI Creative Commons
Wenming Wang,

Liling Xie,

Lin Zhao

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 612 - 612

Published: March 7, 2025

Alum mining leads to significant heavy metal and acid pollution within soils. Phytoremediation is a common strategy used treat alum mine soils, but its efficiency frequently compromised by the alum-mining-induced impairment of plant growth. To improve strength plants against pollution, this study constructed artificial yeast strain ScHB (heavy metal-binding protein-containing Saccharomyces cerevisiae) expressing de novo designed protein HBGFP green fluorescence protein) investigated effect on phytoremediation soils with soil physiochemical assays quantification. This was composed an N-terminal signal peptide, HB metal-binding) domain, GFP (green as well C-terminal glycolphosphatidylinositol-anchoring fragment. The exposure surface increased growth rate cells enhanced cadmium capture from cadmium-containing medium. After culturing Medicago sativa in for 30 days, remarkably plants’ average height 17.5 cm 27.9 their biomass 3.03 g/plant 4.35 g/plant, increasing accumulation antioxidant agents plants. Moreover, strongly improved quality, increase pH values 5.47 6.21 6.9, levels organic matter, total nitrogen, available phosphorus, living bacteria. Furthermore, efficiently abilities remove metals, decreasing cadmium, lead, chromium, copper 90%, 86%, 97%, 88%, respectively. developed genetic engineering method mining.

Language: Английский

Citations

0