N6‐Methyladenosine‐Modified circSMAD4 Prevents Lumbar Instability Induced Cartilage Endplate Ossification
Hanwen Li,
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Yingchuang Tang,
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Sihan Hu
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et al.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 12, 2025
Lumbar
instability
causes
cartilage
endplate
ossification
and
intervertebral
disc
degeneration.
In
this
study,
it
is
determined
that
circSMAD4,
a
Yap1-related
circRNA,
stably
downregulated
under
abnormal
stress.
vitro,
circSMAD4
knockdown
resulted
in
Yap1
mRNA
degradation,
whereas
overexpression
increased
expression
nuclear
translocation.
Hence,
the
stabilization
of
essential
for
maintaining
homeostasis
Furthermore,
transcriptome
sequencing
mass
spectrometry
analysis
revealed
METTL14-mediated
N6-methyladenosine
(m6A)
modification
can
stabilize
expression.
Moreover,
shown
to
regulate
through
m6A
reader
IGF2BP1.
The
IGF2BP1
functions
translocate
into
nucleus,
which
protects
chondrocytes
from
Finally,
local
injection
an
AAV5-containing
plasmid
successfully
rescued
LSI-induced
degeneration,
wasn't
observed
knockout
mice.
These
findings
suggest
m6A-modified
translocation,
thus
preventing
degeneration
may
become
potential
therapeutic
tool
managing
instability-induced
Language: Английский
Biomaterial-based circular RNA therapeutic strategy for repairing intervertebral disc degeneration
Biomedical Technology,
Journal Year:
2024,
Volume and Issue:
9, P. 100057 - 100057
Published: Nov. 21, 2024
Language: Английский
Circular RNAs as multifaceted molecular regulators of vital activity and potential biomarkers of aging
Epigenomics,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 11
Published: Nov. 26, 2024
Aging
presents
a
significant
challenge
to
health
and
social
care
systems
due
the
increasing
proportion
of
elderly
population.
The
identification
reliable
biomarkers
assess
progression
aging
remains
an
unresolved
question.
Circular
RNAs
(circRNAs)
are
single-stranded
covalently
closed
RNAs.
They
have
been
found
regulate
various
biological
processes.
CircRNAs
present
in
human
fluids,
relatively
stable,
accumulate
with
age,
making
them
promising
as
aging.
Current
information
on
expression
circRNAs
was
analyzed
using
scientific
databases.
In
this
review,
we
identified
key
stages
study
during
summarized
current
understanding
their
biogenesis.
By
focusing
role
processes
that
contribute
–
such
genomic
stability,
metabolism,
cell
death,
signaling
pathways
hypothesize
may
drive
process
through
age-related
accumulation
resultant
deregulation.
Examples
differential
species,
including
humans,
provided.
This
review
highlights
importance
finding
novel
epigenetic
aging,
beyond
already
molecules
(circFOXO3,
circRNA100783,
circPVT1),
potential
therapeutic
target
for
treatment
age-associated
diseases.
Language: Английский
A Binary siRNA‐Loaded Tetrahedral DNA Nanobox for Synergetic Anti‐Aging Therapy
Small,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 17, 2024
Extensive
accumulation
of
senescent
cells
contributes
to
organismal
aging,
and
slowing
down
the
process
cellular
senescence
may
ameliorate
age-related
pathologies.
Targeted
inhibition
mechanistic
target
rapamycin
complex
1
(mTORC1)
is
found
suppress
conversion
senescence.
The
regulatory-associated
protein
mTOR
(Raptor),
a
key
component
mTORC1,
has
been
implicated
as
important
in
aging
process,
its
druggability
deserves
be
investigated.
Due
high
efficiency
convenience
drug
construction,
siRNA
shows
great
potential
silencing
Raptor
expression
via
RNA
interfering
therapy.
Here,
we
developed
functionalized
anti-aging
nanoplatform
based
on
tetrahedral
DNA
nanostructures
(TDNs)
encapsulating
targeting
for
synergistic
therapy,
named
siR-TDN
Language: Английский