Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Nov. 13, 2024
Abstract
Background
The
antibacterial
and
immunomodulatory
activities
of
bacteriocins
make
them
attractive
targets
for
development
as
anti-infective
drugs.
Although
the
importance
enteric
nervous
system
(ENS)
in
struggle
against
infections
intestine
has
been
demonstrated,
whether
it
is
involved
mechanisms
poorly
defined.
Results
Here,
we
demonstrated
that
bacteriocin
Microcin
J25
(J25)
significantly
alleviated
diarrhea
intestinal
inflammation
piglets
caused
by
enterotoxigenic
Escherichia
coli
(ETEC)
infection.
Mechanistically,
macrophage
levels
were
downregulated
after
treatment,
this
was
replicated
a
mouse
model.
Omics
analysis
validation
screening
revealed
treatment
induced
significant
changes
dopaminergic
neuron
pathway,
but
little
change
microbial
structure.
alleviation
may
occur
down-regulating
dopamine
receptor
(DR)
D1
downstream
DAG-PKC
thus
inhibiting
arachidonic
acid
decomposition,
inhibition
macrophages
through
up-regulation
DRD5
cAMP-PKA
NF-κB.
Conclusions
Our
studies’
findings
provide
insight
into
possible
roles
ENS
ETEC
infection,
providing
more
sophisticated
foundational
understanding
developing
application
potential
J25.
Journal of Medicinal Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Antimicrobial
peptides
(AMPs)
show
potential
as
antibiotic
alternatives
for
bacterial
infections;
nevertheless,
the
susceptibility
to
proteases
limits
their
broader
utilization.
This
study
developed
engineered
lipopeptides
using
antienzymolysis
modifications
and
cysteine
(Cys)-dimerization
strategy.
As
key
parameters
functioning
of
AMPs,
hydrophobicity
positive
charges
were
concentrated
within
peptide
sequence
by
adjusting
intermolecular
disulfide
bond
placement
distribution
effects.
Their
centralization
in
induces
a
differential
propensity
toward
membranes.
Positive
charge-concentrated
dimeric
lipopeptide
(C-C
iMeta,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 23, 2025
Inflammatory
bowel
disease
(IBD)
represents
a
significant
challenge
to
global
health,
characterized
by
intestinal
inflammation,
impaired
barrier
function,
and
dysbiosis,
with
limited
therapeutic
options.
In
this
study,
we
isolated
novel
strain
of
Bacillus
subtilis
(B.
subtilis)
observed
promising
effects
in
protecting
against
disruption
the
gut
barrier.
Our
findings
indicate
that
enhancement
function
is
primarily
attributed
its
metabolites.
We
identified
metabolite,
2-hydroxy-4-methylpentanoic
acid
(HMP),
derived
from
B.
subtilis,
significantly
improved
function.
also
show
growth
arrest
DNA
damage
45A
(GADD45A)
key
regulator
mucosal
integrity,
which
activated
HMP
subsequently
activates
downstream
Wnt/β-catenin
pathway.
potentially
contribute
development
probiotics-derived
metabolites
or
targeted
"postbiotics"
as
therapeutics
for
treatment
prevention
IBD
other
diseases
associated
dysfunction.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(37), P. 25446 - 25464
Published: Sept. 6, 2024
The
alarming
rise
in
global
antimicrobial
resistance
underscores
the
urgent
need
for
effective
antibacterial
drugs.
Drawing
inspiration
from
bacterial-entrapment
mechanism
of
human
defensin
6,
we
have
fabricated
biomimetic
peptide
nanonets
composed
multiple
functional
fragments
bacterial
eradication.
These
are
designed
to
address
challenges
through
a
dual-approach
strategy.
First,
resulting
nanofibrous
networks
trap
bacteria
and
subsequently
kill
them
by
loosening
membrane
structure,
dissipating
proton
motive
force,
causing
metabolic
perturbations.
Second,
these
trapped
clusters
reactivate
macrophages
scavenge
enhanced
chemotaxis
phagocytosis
via
PI3K-AKT
signaling
pathway
ECM-receptor
interaction.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 13, 2025
The
emergence
of
multidrug-resistant
(MDR)
pathogens,
coupled
with
the
limited
effectiveness
existing
antibiotics
in
eradicating
biofilms,
presents
a
significant
threat
to
global
health
care.
This
critical
situation
underscores
urgent
need
for
discovery
and
development
antimicrobial
agents.
Recently,
peptide-derived
nanomaterials
have
shown
promise
combating
such
infections.
Amino
acid
noncovalent
forces,
notably
π–π
stacking
electrostatic
interactions,
remain
underutilized
guiding
coassembly
peptides
into
bacteriostatic
nanomaterials.
Thus,
we
constructed
dimeric
nanopeptide
system
using
disulfide
bonds
cysteine.
self-assembly
nanofibers
was
realized
by
interaction
aromatic
amino
acids
(Trp,
Phe,
Pyr)
attraction
between
oppositely
charged
(Asp
Arg).
optimal
peptide
2D2W
exhibits
potent
antibacterial
activity
against
resistant
bacteria
is
nontoxic.
Mechanistically,
penetrated
outer
membrane
after
adsorption,
resulting
plasma
depolarization,
homeostatic
disruption,
ultimately
bacterial
death.
In
mouse
model
peritonitis,
demonstrated
efficacy
vivo
treatment
conclusion,
design
nanopeptides
co-driven
intermolecular
forces
provides
promising
avenue
high-performance
These
advances
may
also
facilitate
application
advancement
peptide-based
agents
clinical
practice.
Journal of Agricultural and Food Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 13, 2025
Glyphosate-based
herbicide
(GBH),
a
feed
contaminant,
has
been
proven
to
impair
the
growth
and
development
of
humans
animals.
Previous
research
revealed
that
maternal
toxin
exposure
during
pregnancy
could
cause
permanent
fetal
changes
by
epigenetic
modulation.
However,
there
was
insufficient
evidence
involvement
DNA
methylation
in
GBH
exposure-induced
intestinal
health
offspring.
Here,
we
established
pregnant
sow
models
investigate
effects
on
newborn
piglets.
The
results
showed
gestational
compromises
function
piglets
as
well
decreases
mRNA
expression
Dnmt1
Dnmt3b
jejunum.
Further
RRBS
analysis
genomic
hypomethylation
jejunum,
differentially
methylated
regions
were
enriched
pathways
food
digestion
related
GO
terms.
Additionally,
integrative
methylome
transcriptome
identified
23
genes
showing
inverse
correlations
indicated
underlying
injury
mechanisms
upon
GBH.
These
findings
provide
new
insights
fundamental
knowledge
into
possible
offspring
induced
pregnancy,
which
drives
manufacturers
develop
low-toxicity
ensure
safety
human
health.
Journal of Medicinal Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 25, 2025
The
rise
of
antimicrobial
resistance
(AMR)
in
Gram-negative
bacteria,
including
Escherichia
coli
(E.
coli),
poses
a
major
public
health
threat.
This
study
aimed
to
address
the
limitations
existing
peptides
(AMPs)
by
designing
hybrid
with
enhanced
targeting
and
antibacterial
potency.
Eight
heptapeptide
sequences
were
identified
through
phage
display
screening
hybridized
WP
(WKKIWKPGIKKWIK),
peptide
exhibiting
weak
activity
against
bacteria.
systematically
evaluated
for
their
activity,
specificity,
biocompatibility.
SWP
exhibited
superior
particularly
E.
K88
(TI
=
2.378),
demonstrated
specific
binding
thymidylate
kinase
(TMK),
key
bacterial
enzyme.
In
vivo
studies
employing
mouse
peritonitis
model
confirmed
SWP's
ability
reduce
loads
mitigate
tissue
damage
while
maintaining
excellent
These
findings
underscore
as
promising
candidate
development
targeted
agents
specificity
stability
pathogens.
Journal of Crustacean Biology,
Journal Year:
2025,
Volume and Issue:
45(1)
Published: Jan. 10, 2025
Abstract
The
Chinese
mitten
crab,
Eriocheir
sinensis
H.
Milne
Edwards,
1853,
is
a
migratory
species
critical
to
both
aquatic
ecosystems
and
aquaculture
in
China.
We
investigated
the
construction
of
migration
passageways
through
floodgates
examined
length-weight
relationships
E.
Yangtze
River.
In
response
declining
numbers
due
fishing
infrastructural
barriers,
we
developed
implemented
two
types
crab
at
Sanchahe
River
Gate
Nanjing,
China:
vertical
made
burlap
cloth
horizontal
passageway
constructed
from
stainless
steel
canvas.
observed
that
crabs
could
successfully
use
these
passageways,
with
increased
activity
night.
analyzed
490
crabs,
comparing
individuals
ponds.
Results
showed
exhibited
isometric
growth,
whereas
pond
displayed
negative
allometric
growth
patterns.
Additionally,
were
generally
wider
same
length
than
their
counterparts.
findings
highlight
effectiveness
facilitating
provide
new
insights
into
dynamics
sinensis.
This
research
contributes
our
understanding
offers
practical
guidance
for
future
conservation
efforts.
