Single intranasal immunization with attenuated Wuhan-like SARS-CoV-2 provides highly effective cross-protection against Delta and Omicron variants of concern DOI Creative Commons
Е. Б. Файзулоев, A. V. Gracheva, E. R. Korchevaya

et al.

Journal of microbiology epidemiology immunobiology, Journal Year: 2024, Volume and Issue: 101(1), P. 36 - 51

Published: March 9, 2024

Introduction. Despite the end of COVID-19 pandemic, problem vaccine prevention this disease appears highly relevant. The emergence and widespread distribution Omicron SARS-CoV-2 variant concern (VOC) its sublineages has dramatically reduced efficacy vaccination. possible approach to solving is develop a nasal live attenuated capable activating humoral, mucosal, cell-mediated immunity, providing prolonged immune response cross-protection against different VOCs. aim study was determine immunization with cold-adapted Wuhan-like D-D2 strain homologous heterologous challenges. Materials methods. conducted on an animal model coronavirus pneumonia in golden Syrian hamsters. assessed by comparing dynamics weight, viral load organs histopathological changes lungs immunized unimmunized animals. Results. Single intranasal hamsters showed high immunogenicity: seroconversion evident all provides effective protection development productive infection when challenged both ancestral virus strains related Delta (AY.122) (sublineages BA.1.1 BA.5.2) variants. Conclusion. attenuation promising strategy for vaccine.

Language: Английский

Single intranasal immunization with attenuated Wuhan-like SARS-CoV-2 provides highly effective cross-protection against Delta and Omicron VOCs DOI Creative Commons
Е. Б. Файзулоев, A. V. Gracheva, E. R. Korchevaya

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 9, 2023

Abstract Despite the end of COVID-19 pandemic, problem vaccine prevention this disease appears highly relevant. The emergence and widespread distribution Omicron SARS-CoV-2 variant concern (VOC) its sublineages has dramatically reduced efficacy vaccination with licensed vaccines. possible approach to solving is develop a nasal live attenuated (LAV) capable activating humoral, mucosal, cell-mediated immunity, providing prolonged immune response cross-protection against different VOCs. aim present study was determine immunization cold-adapted Wuhan-like D-D2 strain homologous heterologous challenges. Single intranasal golden Syrian hamsters showed high immunogenicity: seroconversion evident in all immunized animals. absence infectious virus lung, passages, brains animals allows us characterize immunity as "sterile" consistent viral RNA concentration organs on fourth-day post-challenge. Comparison data reproduction organs, weight dynamics, morphological changes lungs unimmunized that provides effective protection infection development pneumonia when challenged both ancestral Delta (B.1.617.2.122) (sublineages BA.1 BA.5.2) variants. Thus, variants have great potential for vaccines their basis.

Language: Английский

Citations

1
Single intranasal immunization with attenuated Wuhan-like SARS-CoV-2 provides highly effective cross-protection against Delta and Omicron variants of concern DOI Creative Commons
Е. Б. Файзулоев, A. V. Gracheva, E. R. Korchevaya

et al.

Journal of microbiology epidemiology immunobiology, Journal Year: 2024, Volume and Issue: 101(1), P. 36 - 51

Published: March 9, 2024

Introduction. Despite the end of COVID-19 pandemic, problem vaccine prevention this disease appears highly relevant. The emergence and widespread distribution Omicron SARS-CoV-2 variant concern (VOC) its sublineages has dramatically reduced efficacy vaccination. possible approach to solving is develop a nasal live attenuated capable activating humoral, mucosal, cell-mediated immunity, providing prolonged immune response cross-protection against different VOCs. aim study was determine immunization with cold-adapted Wuhan-like D-D2 strain homologous heterologous challenges. Materials methods. conducted on an animal model coronavirus pneumonia in golden Syrian hamsters. assessed by comparing dynamics weight, viral load organs histopathological changes lungs immunized unimmunized animals. Results. Single intranasal hamsters showed high immunogenicity: seroconversion evident all provides effective protection development productive infection when challenged both ancestral virus strains related Delta (AY.122) (sublineages BA.1.1 BA.5.2) variants. Conclusion. attenuation promising strategy for vaccine.

Language: Английский

Citations

0