Determination of eight phthalate metabolites in urine of pregnant women and evaluation of neonatal birth outcomes based on solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry DOI
Zihao Wang, Meijuan Xu,

Bingchu Li

et al.

Chinese Journal of Chromatography, Journal Year: 2025, Volume and Issue: 43(1), P. 60 - 67

Published: Jan. 1, 2025

Phthalates (PAEs) are endocrine-disrupting chemicals that widely present in everyday life and enter the human body through various pathways. The release of PAEs into environment pathways include leaching, evaporation, abrasion, use personal care products exposes humans to via ingestion, inhalation, dermal absorption. Pregnant women, as a particularly vulnerable population, risk adverse newborn growth development when exposed PAEs. While concentrations urine reflect recent exposure levels humans, urinary phthalate metabolites (mPAEs) commonly used biomarkers internal owing relatively short biological half-lives (<24 h). In this study, we developed solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS) method for simultaneously detecting eight mPAEs pregnant women. Urine samples were enzymatically hydrolyzed with β-glucosidase then purified using Bond Elut Plexa SPE column, subsequent elution, concentration, redissolved performed prior HPLC-MS/MS. Separation was achieved an Agilent Eclipse Plus C18 column (100 mm×3 mm, 3.5 μm), gradient elution 0.1% acetic acid aqueous solution acetonitrile mobile phases. Multiple reaction monitoring (MRM) mode detection, quantification internal-standard method. Good linearities obtained range 0.1-200 ng/mL mPAEs, limits detection (LODs) (LOQs) 0.015-0.048 0.050-0.160 ng/mL, respectively. exhibited recoveries 80.2%-99.7% at three spiked (1, 10, 50 ng/mL). This subsequently analyze 497 women from Ezhou Maternity Child Health Care Hospital. participants widespread PAEs, monobutyl (MBP) showing highest median level 104.46 monobenzyl (MBzP) lowest (0.22 addition, study assessed neonatal birth outcomes. Linear regression modeling revealed gestational age decreased by 0.11 weeks (95% confidence interval (CI): -0.18--0.03) every natural-log (ln) increase monoethyl (MEP) woman. Moreover, weight 39.28 g CI: -76.48--2.09) 39.62 -73.73--5.52), ln mono(2-ethyl-5-oxohexyl) (MEOHP) mono(2-ethylhexyl) (MECPP) levels, is characterized its simplicity, low LODs, high accuracy, precision. provides clear evidence PAE during pregnancy negatively affects measuring linking these findings Further large-scale cohort studies needed validate findings, along mechanistic animal models or in-vitro systems elucidate which contribute

Language: Английский

Gene-environment interactions within a precision environmental health framework DOI Creative Commons
Alison A. Motsinger‐Reif, David M. Reif, Farida S. Akhtari

et al.

Cell Genomics, Journal Year: 2024, Volume and Issue: 4(7), P. 100591 - 100591

Published: June 25, 2024

Understanding the complex interplay of genetic and environmental factors in disease etiology role gene-environment interactions (GEIs) across human development stages is important. We review state GEI research, including challenges measuring advantages analysis understanding mechanisms. discuss evolution studies from candidate to genome-wide interaction (GWISs) multi-omics mediating effects. advancements methods importance large-scale datasets. also address translation findings into precision health (PEH), showcasing real-world applications healthcare prevention. Additionally, we highlight societal considerations justice, return results participants, data privacy. Overall, underscore significance for prediction prevention advocate integrating exposome PEH omics studies.

Language: Английский

Citations

10
Determination of eight phthalate metabolites in urine of pregnant women and evaluation of neonatal birth outcomes based on solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry DOI
Zihao Wang, Meijuan Xu,

Bingchu Li

et al.

Chinese Journal of Chromatography, Journal Year: 2025, Volume and Issue: 43(1), P. 60 - 67

Published: Jan. 1, 2025

Phthalates (PAEs) are endocrine-disrupting chemicals that widely present in everyday life and enter the human body through various pathways. The release of PAEs into environment pathways include leaching, evaporation, abrasion, use personal care products exposes humans to via ingestion, inhalation, dermal absorption. Pregnant women, as a particularly vulnerable population, risk adverse newborn growth development when exposed PAEs. While concentrations urine reflect recent exposure levels humans, urinary phthalate metabolites (mPAEs) commonly used biomarkers internal owing relatively short biological half-lives (<24 h). In this study, we developed solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS) method for simultaneously detecting eight mPAEs pregnant women. Urine samples were enzymatically hydrolyzed with β-glucosidase then purified using Bond Elut Plexa SPE column, subsequent elution, concentration, redissolved performed prior HPLC-MS/MS. Separation was achieved an Agilent Eclipse Plus C18 column (100 mm×3 mm, 3.5 μm), gradient elution 0.1% acetic acid aqueous solution acetonitrile mobile phases. Multiple reaction monitoring (MRM) mode detection, quantification internal-standard method. Good linearities obtained range 0.1-200 ng/mL mPAEs, limits detection (LODs) (LOQs) 0.015-0.048 0.050-0.160 ng/mL, respectively. exhibited recoveries 80.2%-99.7% at three spiked (1, 10, 50 ng/mL). This subsequently analyze 497 women from Ezhou Maternity Child Health Care Hospital. participants widespread PAEs, monobutyl (MBP) showing highest median level 104.46 monobenzyl (MBzP) lowest (0.22 addition, study assessed neonatal birth outcomes. Linear regression modeling revealed gestational age decreased by 0.11 weeks (95% confidence interval (CI): -0.18--0.03) every natural-log (ln) increase monoethyl (MEP) woman. Moreover, weight 39.28 g CI: -76.48--2.09) 39.62 -73.73--5.52), ln mono(2-ethyl-5-oxohexyl) (MEOHP) mono(2-ethylhexyl) (MECPP) levels, is characterized its simplicity, low LODs, high accuracy, precision. provides clear evidence PAE during pregnancy negatively affects measuring linking these findings Further large-scale cohort studies needed validate findings, along mechanistic animal models or in-vitro systems elucidate which contribute

Language: Английский

Citations

0