Ex vivo study on prebiotic & choline combination to modulate gut bacteria, enhance choline bioavailability, and reduce TMA production DOI Open Access

Ying Qi Goh,

Guoxiang Cheam,

Mingyue Yeong

et al.

Microbiome Research Reports, Journal Year: 2025, Volume and Issue: 4(2)

Published: May 6, 2025

Aim: Choline is a universal methyl group donor, playing an essential role in DNA methylation, signaling pathways, and the transport metabolism of lipids. The primary source choline intake diet, chronic deficiency has been associated with dementia, cardiovascular disease, liver disease. bioavailability can be diminished by gut microbes that express trimethylamine-lyase (cutC ), enzyme converts into trimethylamine (TMA), precursor for TMA N-oxide (TMAO), which increased risk diseases. Gut microbiota modulation achieved prebiotics such as galactooligosaccharides, inulin, fructooligosaccharides. aim our study to use modulate enhance minimize production. Methods: We employed ex vivo microcosm system consisting healthy human stool samples different measured levels targeted metabolomics. Shotgun metagenomic profiling was also performed investigate alternation composition during prebiotic interventions. Results: Our showed conversion dependent on derivative supplementing galactooligosaccharides (GOS) reduces this conversion. enriched from genus Dialister , whereas GOS supplementation led increase Blautia reduction Clostridia populations. Loss reduced subset Clostridium species, citroniae known encode cutC gene. abundance enhanced chorismate biosynthesis pathway, while supported tryptophan methionine pathways. Conclusion: This first identify combination potential strategy its metabolites order improve disease etiology.

Language: Английский

Ex vivo study on prebiotic & choline combination to modulate gut bacteria, enhance choline bioavailability, and reduce TMA production DOI Open Access

Ying Qi Goh,

Guoxiang Cheam,

Mingyue Yeong

et al.

Microbiome Research Reports, Journal Year: 2025, Volume and Issue: 4(2)

Published: May 6, 2025

Aim: Choline is a universal methyl group donor, playing an essential role in DNA methylation, signaling pathways, and the transport metabolism of lipids. The primary source choline intake diet, chronic deficiency has been associated with dementia, cardiovascular disease, liver disease. bioavailability can be diminished by gut microbes that express trimethylamine-lyase (cutC ), enzyme converts into trimethylamine (TMA), precursor for TMA N-oxide (TMAO), which increased risk diseases. Gut microbiota modulation achieved prebiotics such as galactooligosaccharides, inulin, fructooligosaccharides. aim our study to use modulate enhance minimize production. Methods: We employed ex vivo microcosm system consisting healthy human stool samples different measured levels targeted metabolomics. Shotgun metagenomic profiling was also performed investigate alternation composition during prebiotic interventions. Results: Our showed conversion dependent on derivative supplementing galactooligosaccharides (GOS) reduces this conversion. enriched from genus Dialister , whereas GOS supplementation led increase Blautia reduction Clostridia populations. Loss reduced subset Clostridium species, citroniae known encode cutC gene. abundance enhanced chorismate biosynthesis pathway, while supported tryptophan methionine pathways. Conclusion: This first identify combination potential strategy its metabolites order improve disease etiology.

Language: Английский

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