Molecular Targets of Minor Cannabinoids in Breast Cancer: In Silico and In Vitro Studies

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(9), P. 1245 - 1245

Published: Sept. 21, 2024

Background: Breast cancer therapy has been facing remarkable changes. Classic treatments are now combined with other therapies to improve efficacy and surpass resistance. Indeed, the emergence of resistance demands development novel therapeutic approaches. Due key estrogen signaling, receptor-positive (ER+) breast treatment always focused on aromatase inhibition ER modulation. Lately, effects phytocannabinoids, mainly Δ9-tetrahydrocannabinol (THC) cannabidiol (CBD), have evaluated in different cancers, including breast. However, Cannabis sativa contains more than 120 phytocannabinoids less researched understood. Methods: Here, we evaluated, both silico vitro, ability 129 modulate important molecular targets ER+ cancer: aromatase, ER, androgen receptor (AR). Results: In results suggested that some cannabinoids may inhibit act as ERα antagonists. Nine selected showed, potential either antagonists inverse agonist properties, or agonists. Moreover, these were considered weak inhibitors AR action. Conclusions: Overall, present, for first time, a comprehensive analysis actions tumors, pointing out their diseases.

Language: Английский

New Cannabinoids and Chlorin-Type Metabolites from the Flowers of Cannabis sativa L.: A Study on Their Neuroblastoma Activity

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(4), P. 521 - 521

Published: April 3, 2025

Background/Objectives: Cannabis sativa has been utilized for medical purposes thousands of years. It continues to be recognized as a plant with an extensive variety medicinal and nutraceutical uses today. In this study, chemical investigation the flowers C. isolated by using chromatographic techniques led isolation eleven compounds. These purified compounds were evaluated antitumor activity against SK-N-SH neuroblastoma cells. Methods: The techniques. Their structures identified examination spectroscopic methods, including 1D (1H, 13C, DEPT) 2D (COSY, HSQC, HMBC, NOESY) nuclear magnetic resonance (NMR) spectra mass spectrum, together comparison those reported previously in literature. evaluation toxicity on cells was performed MTT method. Results: Eleven from sativa, two new compounds, namely cannabielsoxa (1), 132-hydroxypheophorbide c ethyl ester (2), six known cannabinoids (6–11), first chlorin-type compounds: pyropheophorbide A (3), b (4), ligulariaphytin (5) plant. results also demonstrated that cannabinoid had stronger inhibitory effects than Conclusions: biological activities showed 4–10 could considered potential neuroblastomas. This is highlighted docking analysis. Additionally, study suggest these have developed into antineuroblastoma products.

Language: Английский