A‐to‐I‐Edited miR‐1304‐3p Inhibits Glycolysis and Tumor Growth of Esophageal Squamous Cell Carcinoma by Inactivating Wnt5a/ROR2 Signaling

Molecular Carcinogenesis, Journal Year: 2025, Volume and Issue: 64(3), P. 552 - 564

Published: Jan. 6, 2025

ABSTRACT A‐to‐I RNA editing is a pervasive mechanism in the human genome that affects regulation of gene expression and closely associated with pathogenesis numerous diseases. This study elucidates regulatory edited miR‐1304‐3p esophageal squamous cell carcinoma (ESCC). Western blot, immunohistochemistry, RT‐qPCR assays were employed to quantify protein mRNA expression. Colony formation, Edu, wound healing, Transwell applied determine miRNA function. Glycolysis was assessed using glucose uptake lactate production assay. A dual‐luciferase reporter assay confirmed downstream targets miRNA, xenograft demonstrated efficacy miRNA. The level observed increase KYSE180 KYSE140 ESCC cells following ADAR1 treatment. Following editing, function progression underwent reversal, shifting from carcinogenic inhibitory. Wild‐type (WT) IRS1, whereas version ROR2. WT miR‐1304‐3p, but not version, suppressed tumor‐suppressive effect IRS1 ESCC. Conversely, ROR2, specific target acted as tumor promoter Furthermore, can inhibit glycolysis inactivate Wnt5a/ROR2 signaling pathway alters by changing its gene. hinders development inhibiting inactivating pathway.

Language: Английский

Unfolded protein responses: Dynamic machinery in wound healing

Pharmacology & Therapeutics, Journal Year: 2025, Volume and Issue: unknown, P. 108798 - 108798

Published: Jan. 1, 2025

Language: Английский

Downregulation of SMAD2 and SMAD4 is associated with poor prognosis and shorter survival in esophageal squamous cell carcinoma

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: March 3, 2025

Language: Английский