A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface DOI Open Access

Anca Emanuela Minuti,

Luminiţa Lăbușcă, Dumitru-Daniel Herea

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 24(1), P. 430 - 430

Published: Dec. 27, 2022

Preparing biological specimens for scanning electron microscopy (SEM) can be difficult to implement, as it requires specialized equipment and materials well the training of dedicated personnel. Moreover, procedure often results in damage samples analyzed. This work presents a protocol preparation evaluate adherence nanomaterials on cell surface using SEM. To this end, we used silicon wafers substrate grow cells replaced steps such critical point drying order make method quicker easier perform. The new was tested two different types cells, i.e., human osteosarcoma adipose-derived mesenchymal stem proved that grossly preserve integrity estimate nanomaterials' interaction with surfaces.

Language: Английский

Co-Fe nanowires: a viable strategy for destroying cancer cells via a magnetomechanical effect DOI Creative Commons
Anca-Emanuela Minuti, Cristina Stavilă,

Adrian Ghemes

et al.

Journal of Nanoparticle Research, Journal Year: 2025, Volume and Issue: 27(5)

Published: April 21, 2025

Language: Английский

Citations

0
A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface DOI Open Access

Anca Emanuela Minuti,

Luminiţa Lăbușcă, Dumitru-Daniel Herea

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 24(1), P. 430 - 430

Published: Dec. 27, 2022

Preparing biological specimens for scanning electron microscopy (SEM) can be difficult to implement, as it requires specialized equipment and materials well the training of dedicated personnel. Moreover, procedure often results in damage samples analyzed. This work presents a protocol preparation evaluate adherence nanomaterials on cell surface using SEM. To this end, we used silicon wafers substrate grow cells replaced steps such critical point drying order make method quicker easier perform. The new was tested two different types cells, i.e., human osteosarcoma adipose-derived mesenchymal stem proved that grossly preserve integrity estimate nanomaterials' interaction with surfaces.

Language: Английский

Citations

11