Fluorine–Nitrogen Codoped Carbon Dots for Visualization Imaging of Nucleic Acids via Two-Photon Fluorescence Lifetime Microscopy DOI

Yong Guo,

Zhenlong Huang, Luwei Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Fluorescence imaging is a key tool for visualizing the morphology and dynamics of nucleic acids (DNA RNA) in living cells to understand their role regulating growth, development, reproduction organisms. However, effective probes capable simultaneously targeting both DNA RNA, as well tools analyzing distribution relative ratios organisms, are currently lacking. Therefore, fluorine-nitrogen codoped carbon dots with two-photon absorption (F-NCDs) were synthesized by hydrothermal method exhibited stable fluorescence, good biocompatibility, fluorescence lifetime sensitive RNA). The as-prepared F-NCDs act probe quantify distinguish RNA nucleus via multicolor microscopy (TP-FLIM). was particularly tracking changes DNA/RNA plant cell nuclei (onion root tips) during different division stages distinguishing animal tissues (zebrafish). development provides insights into preparation offers an visualization TP-FLIM dynamically study function genetic material various life activities.

Language: Английский

Fluorine–Nitrogen Codoped Carbon Dots for Visualization Imaging of Nucleic Acids via Two-Photon Fluorescence Lifetime Microscopy DOI

Yong Guo,

Zhenlong Huang, Luwei Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Fluorescence imaging is a key tool for visualizing the morphology and dynamics of nucleic acids (DNA RNA) in living cells to understand their role regulating growth, development, reproduction organisms. However, effective probes capable simultaneously targeting both DNA RNA, as well tools analyzing distribution relative ratios organisms, are currently lacking. Therefore, fluorine-nitrogen codoped carbon dots with two-photon absorption (F-NCDs) were synthesized by hydrothermal method exhibited stable fluorescence, good biocompatibility, fluorescence lifetime sensitive RNA). The as-prepared F-NCDs act probe quantify distinguish RNA nucleus via multicolor microscopy (TP-FLIM). was particularly tracking changes DNA/RNA plant cell nuclei (onion root tips) during different division stages distinguishing animal tissues (zebrafish). development provides insights into preparation offers an visualization TP-FLIM dynamically study function genetic material various life activities.

Language: Английский

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