Effects of Lupeol on Intestinal Anastomosis After Experimental Intestinal Ischemia-Reperfusion Injury in Rats

Drug Design Development and Therapy, Journal Year: 2025, Volume and Issue: Volume 19, P. 479 - 490

Published: Jan. 1, 2025

Intestinal ischemia/reperfusion (I/R) injury can occur in a wide variety of diseases and surgeries. If necessary, the blood flow should be restored, including re-anastomosis by removing intestines with impaired circulation. In this process, anastomotic strength is as important inflammatory responses oxidative stress. Therefore, we conducted study to investigate effects lupeol on intestinal ischemia-reperfusion injury, not only biochemically histopathologically but also miRNAs. Female rats were randomly divided into six groups. While laparotomy was performed control group (Group C), anastomosis sham S). other groups, superior mesenteric artery clamped for 45 minutes. groups I/R1 L1, intestine transected, end-to-end at 1st hour reperfusion. I/R24 L24, procedure 24th addition, treatment given before reperfusion following 4 days L1 L24. All rats, except group, bursting pressure measured 5th day anastomosis, then all sacrificed. TNF-α, IL-6 levels samples MDA, GSH, caspase-3, miR-29b-3p, miR-34a-5p, miR-495-3p tissues measured, histopathology examined. Lupeol treatment, which statistically significant some parameters, demonstrated positive decreasing TNF, IL-6, histopathological damage increasing GSH pressure. decreased miR-34a-5p expression increased miR-29b-3p expression. protected from I/R its antioxidant anti-inflammatory effects. Besides, it reduced strength. Additionally, expressions altered lupeol.

Language: Английский

<i>Guiera</i> <i>senegalensis</i> Alleviates Ischemia Renal Reperfusion Injury in Albinos Wistar Rats

Open Journal of Pathology, Journal Year: 2025, Volume and Issue: 15(01), P. 16 - 27

Published: Jan. 1, 2025

Therapeutic effect of umbilical cord mesenchymal stem cells on renal ischemia-reperfusion injury

Acta Pharmaceutica, Journal Year: 2025, Volume and Issue: 75(1), P. 103 - 118

Published: March 1, 2025

Abstract Acute kidney injury (AKI) is a growing global health issue with no effective treatments. This study evaluates the therapeutic effects of umbilical cord mesenchymal stem cells (UC-MSCs) on AKI caused by ischemia-reperfusion (IRI) in mice. Thirty mice were divided into sham group, an IRI and MSC-treated group. Renal function was assessed, histological analysis, immunofluorescence, real-time PCR used to evaluate renal damage, inflammatory cell presence, cytokine expression (TNF-α, IL-6, IL-10). Results showed that MSC treatment reduced decreased pro-inflammatory cytokines IL-6), increased anti-inflammatory IL-10, promoted repair homing sites. Thus, MSCs may mitigate reducing inflammation enhancing repair.

Language: Английский