New insights into the influence of encapsulation materials on the feasibility of ultrasonic-assisted encapsulation of Mosla chinensis essential oil

Ultrasonics Sonochemistry, Journal Year: 2024, Volume and Issue: 103, P. 106787 - 106787

Published: Jan. 30, 2024

The study aimed to estimate the feasibility of α-cyclodextrin (α-CD), β-cyclodextrin (β-CD), and γ-cyclodextrin (γ-CD) encapsulate Mosla chinensis essential oil (EO) by ultrasonic-assisted method. physical properties variations, stabilization mechanisms, formation processes inclusion complexes (ICs) were investigated using experimental methods. Molecular docking, molecular dynamics (MD) simulation, Scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, gas chromatography–mass spectrometry showed that ICs successfully prepared, which differentially improved thermal stability retained chemical composition EO. dissolution profile Peppas model can be used describe diffuse release mechanism Finally, docking MD simulation theoretically confirmed interaction conformational changes carvacrol (the main active component EO) inside cavity CDs. results indicate hydrogen bonding was primary driving force for spontaneous access cavity. Further, a binding dynamic balance occurs between β-CD, whereas bind away in IC α-CD, γ-CD. comprehensive show medium size β-CD is suitable host molecule EO encapsulation, release, stabilization. A combination theoretical calculations useful pinpoint targeted design optimization cyclodextrin encapsulation small entity molecules. rationally screened as better candidate stabilizing EO, provides an option meaningful path realistic applications.

Language: Английский

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The Role of the Nuclear Factor-Kappa B (NF-κB) Pathway in SARS-CoV-2 Infection

Pathogens, Journal Year: 2024, Volume and Issue: 13(2), P. 164 - 164

Published: Feb. 12, 2024

COVID-19 is a global health threat caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and associated with significant increase in morbidity mortality. The present review discusses nuclear factor-kappa B (NF-κB) activation its potential therapeutical role treating COVID-19. pathogenesis, the major NF-κB pathways, involvement of SARS-CoV-2 have been detailed. Specifically, impact on managing has discussed. As central player immune inflammatory responses, modulating could offer strategic avenue for infection. Understanding pathway’s aid developing treatments against SARS-CoV-2. Further investigations into intricacies are required to reveal effective therapeutic strategies combating infection

Language: Английский

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Preliminary results and a theoretical perspective of co‑treatment using a miR‑93‑5p mimic and aged garlic extract to inhibit the expression of the pro‑inflammatory interleukin‑8 gene

Experimental and Therapeutic Medicine, Journal Year: 2025, Volume and Issue: 29(4), P. 1 - 11

Published: Feb. 25, 2025

The coronavirus disease‑19 (COVID‑19) pandemic has been a very significant health issue in the period between 2020 and 2023, forcing research to characterize severe acute respiratory syndrome 2 (SARS‑CoV‑2) sequences develop novel therapeutic approaches. Interleukin‑6 (IL‑6) IL‑8 are considered targets for COVID‑19 emerging evidence suggested that microRNAs (miRNAs/miRs) serve key role regulating these genes. MiRNAs short, 19‑25 nucleotides length, non‑coding RNAs regulate gene expression at post‑transcriptional level through sequence‑selective recognition of 3'‑untranslated region (3'‑UTR) regulated mRNAs, eventually repressing translation, commonly, via mRNA degradation. For example, among several miRNAs involved regulation 'cytokine storm', miR‑93‑5p can inhibit by directly targeting 3'‑UTR mRNA. In addition, Toll‑like receptor‑4 (TLR4) interleukin‑1 receptor‑associated kinase 4 (IRAK4) expression, thus affecting nuclear factor‑κB (NF‑κB) pathway NF‑κB‑regulated genes, such as IL‑6, IL‑1β other hyper‑expressed genes during storm'. present study, results provided preliminary suggesting miR‑93‑5p‑based miRNA therapeutics could be combined with anti‑inflammatory aged garlic extract (AGE) more effectively expression. human bronchial epithelial IB3‑1 cell line was employed experimental model system. cells were stimulated BNT162b2 vaccine transfected pre‑miR‑93‑5p absence or presence AGE, verify inhibitory effects on BNT162b2‑induced gene. accumulation assessed RT‑qPCR; release protein determined Bio‑Plex assay. possible applications TLR4/NF‑κB agents (such AGE) treating pathologies hyperinflammatory state, COVID‑19, cystic fibrosis diseases, summarized.

Language: Английский

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The Potential of Glucosinolates and Their Hydrolysis Products as Inhibitors of Cytokine Storms

Molecules, Journal Year: 2024, Volume and Issue: 29(20), P. 4826 - 4826

Published: Oct. 11, 2024

A cytokine storm is an intense inflammatory response characterized by the overproduction of proinflammatory cytokines, resulting in tissue damage, and organ dysfunction. Cytokines play a crucial role various conditions, such as coronavirus disease, which immune system becomes overactive releases excessive levels including interleukins, tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ). This anomalous often leads to acute respiratory distress syndrome (ARDS), disseminated intravascular coagulation (DIC), multiple injury (MOI). Glucosinolates are plant secondary metabolites predominantly found

Language: Английский

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Aged Garlic Extract (AGE) and Its Constituent S-Allyl-Cysteine (SAC) Inhibit the Expression of Pro-Inflammatory Genes Induced in Bronchial Epithelial IB3-1 Cells by Exposure to the SARS-CoV-2 Spike Protein and the BNT162b2 Vaccine

Molecules, Journal Year: 2024, Volume and Issue: 29(24), P. 5938 - 5938

Published: Dec. 16, 2024

Garlic (Allium sativum L.) is a species of the onion family (Alliaceae) widely used as food and folk medicine. The objective this study was to determine effects AGE (aged garlic extract) on pro-inflammatory genes relevant COVID-19. To aim, we treated bronchial epithelial IB3-1 cells with SARS-CoV-2 spike protein (S-protein) or COVID-19 BNT162b2 vaccine in absence presence AGE. results obtained demonstrated that potent inhibitor S-protein-induced expression IL-1β, IL-6 IL-8 genes. Bio-Plex analysis reduced release IL-8, which were highly induced by S-protein. No inhibition cells' growth, toxicity pro-apoptotic found AGE-treated cells. one major constituents (S-allyl cysteine, SAC) studied same experimental model systems. SAC able inhibit extracellular confirming S-allyl-cysteine responsible for inhibiting Docking experiments suggest possible mechanism action an interference activity Toll-like receptors (TLRs), particularly TLR4, thereby NF-κB- NF-κB-regulated genes, such These both deserve further efforts verify their SARS-CoV-2-infected

Language: Английский

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Inhibitory effects of SARS-CoV-2 spike protein and BNT162b2 vaccine on erythropoietin-induced globin gene expression in erythroid precursor cells from patients with β-thalassemia

Experimental Hematology, Journal Year: 2023, Volume and Issue: 129, P. 104128 - 104128

Published: Nov. 6, 2023

During the recent COVID-19 pandemic several β-thalassemia patients have been infected by SARS-CoV-2 and most were vaccinated against SARS-CoV-2. Recent studies demonstrate an impact of infection on hematopoietic system. The main objective this study was to verify effects exposure erythroid precursor cells (ErPCs) from Spike protein (S-protein) BNT162b2 vaccine. Erythropoietin (EPO)-cultured ErPCs either untreated or treated with S-protein employed a cellular Biobank developed before pandemic. genotypes β+-IVSI-110/β+-IVSI-110 (one patient), β039/β+-IVSI-110 (three patients) β039/ β039 (two patients). After 5 days treatment BNT162b2, lysates analysed HPLC, for hemoglobin production, isolated RNA assayed RT-qPCR, detection globin gene expression. conclusions results obtained are that vaccine inhibit (a) fetal production β-thalassemic (b) γ-globin mRNA accumulation. In addition, we performed in silico suggesting high affinity (HbF). Remarkably, binding interaction energy comparable angiotensin-converting enzyme 2 (ACE2). Our consistent hypothesis relevant vaccination

Language: Английский

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The anti-SARS-CoV-2 BNT162b2 vaccine suppresses mithramycin-induced erythroid differentiation and expression of embryo-fetal globin genes in human erythroleukemia K562 cells

Experimental Cell Research, Journal Year: 2023, Volume and Issue: 433(2), P. 113853 - 113853

Published: Nov. 7, 2023

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causative of the ongoing disease 2019 (COVID-19) pandemic. SARS-CoV-2 Spike protein (S-protein) plays an important role in early phase infection through efficient interaction with ACE2. S-protein produced by RNA-based COVID-19 vaccines, that were fundamental for reduction viral spread within population and clinical severity COVID-19. However, has been hypothesized to be responsible damaging cells several tissues some side effects vaccines. Considering impact on hematopoietic system, aim this study was verify effect BNT162b2 vaccine erythroid differentiation human K562 cell line, past intensively studied as a model system mimicking steps erythropoiesis. In context, we focused hemoglobin production induced expression embryo-fetal globin genes, are among most features differentiation. We found suppresses mithramycin-induced cells. Reverse-transcription-qPCR Western blotting assays demonstrated suppression associated sharp inhibition α-globin γ-globin mRNA accumulation. Inhibition accumulation ζ-globin ε-globin mRNAs also observed. addition, provide silico studies suggesting direct between Hb Portland, major This thus provides information need great attention possible alteration parameters following and/or vaccination.

Language: Английский

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Sulforaphane Pre-Treatment Prevents Alcohol-Induced Dysfunction in Human and Murine Macrophage Cells in Vitro

Published: Jan. 1, 2024

Language: Английский

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Sulforaphane Pre-Treatment Prevents Alcohol-Induced Dysfunction in Human and Murine Macrophage Cells in Vitro

Published: Jan. 1, 2024

Language: Английский

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Anti-Inflammatory and Neuroprotective Polyphenols Derived from the European Olive Tree, Olea europaea L., in Long COVID and Other Conditions Involving Cognitive Impairment

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11040 - 11040

Published: Oct. 14, 2024

The European olive tree, Olea europaea L., and its polyphenols hold great therapeutic potential to treat neuroinflammation cognitive impairment. This review examines the evidence for anti-inflammatory neuroprotective actions of their in treatment long COVID neurodegenerative diseases such as Alzheimer’s disease (AD), Parkinson’s (PD), multiple sclerosis (MS). Key findings suggest that exhibit antioxidant, anti-inflammatory, neuroprotective, antiviral properties, making them promising candidates intervention, especially when formulated unique combinations. Recommendations future research directions include elucidating molecular pathways through mechanistic studies, exploring implications polyphenol supplementation, conducting clinical trials assess efficacy safety. Investigating synergistic effects with other agents addressing different targets is suggested further exploration. reviewed strengthens translational value conditions involving dysfunction emphasizes novelty new formulations.

Language: Английский

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