The potential of type 2 cystatin in blood feeding and innate immunity of the tick Haemaphysalis doenitzi (Acari: Ixodidae) DOI Creative Commons

Songbo Zhang,

Zhihua Gao,

Yikui Wang

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 16, 2023

Abstract Ticks, which are obligate blood-sucking parasites, transmit a wide range of pathogens during their blood-feeding process. Enzymes and macromolecules that inhibit enzymes play crucial role in tick physiology. In the present study, genes encoding type 2 cystatin were cloned characterized from Haemaphysalis doenitzi , functions innate immunity blood feeding further assessed. Four genes, namely HDcyst-1 HDcyst-2 HDcyst-3 HDcyst-4 successfully tick. Their open reading frames vary length, with sizes 390, 426, 426 base pairs (bp), respectively. Proteinase inhibition assays indicated 0.2 µM displayed minimum 50% inhibitory activity against cathepsin B S. The qPCR results imply could pivotal hemophagia, whereas may be integral to regulation digestion. Our investigation unveiled adult ticks subjected LPS injection or exposed low-temperature stress exhibited elevated expression levels compared control group. RNA interference experiments demonstrated cystatins had significant impact on engorged weight, number eggs laid, hatching rate, mortality H. along an extension duration by days. These discoveries suggest involvement processes. Consequently, emerges as promising candidate for development novel immunosuppressive drugs future.

Language: Английский

Sophoricoside improved Crohn's disease-like colitis by inhibiting intestinal epithelial cell apoptosis through PI3K/AKT signaling DOI Creative Commons
Qingqing Li, Jing Li, L.X. Yin

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 131, P. 111886 - 111886

Published: March 16, 2024

Increased apoptosis of intestinal epithelial cells (IECs) is a significant cause barrier dysfunction in Crohn's disease (CD). Sophoricoside (SOP) an isoflavone glycoside known for its anti-apoptotic properties. The aim this study was to investigate the effects SOP on mice with CD-like colitis and understand underlying mechanisms. Mice treated 2,4,6-trinitrobenzene sulfonic acid (TNBS) were used examine therapeutic effect damage. To further explore SOP's impact IECs protection, vitro colonic organoid model induced by TNF-α utilized. Network pharmacology employed predict relevant pathways molecular processes associated treatment CD. Treatment significantly improved symptoms TNBS mice, as demonstrated reductions Disease Activity Index (DAI), weight loss, colon shortening, macroscopic scores, tissue inflammatory expression pro-inflammatory factors. Our experiments confirmed that protects counteracting apoptosis. Additionally, established reduced inhibiting PI3K/AKT signaling pathway. can reduce through inhibition pathway, thereby protecting barrier. This first illustrate how ameliorates barrier, suggesting has potential clinical application treating

Language: Английский

Citations

7
Sophoricoside Inhibited Glioblastoma Cell Progression Through Activated AMP‐Activated Protein Kinase (AMPK) DOI Open Access
Changquan Wang,

Xuehui Xiong,

Chang Li

et al.

Molecular Carcinogenesis, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

ABSTRACT Glioblastoma (GBM) is the most common malignant primary brain tumor, with a mean survival of less than 2 years. Unique structures and microenvironment, including blood−brain barriers, put great challenges on clinical drug development. Sophoricoside (Sop), an isoflavone glycoside isolated from seeds Sophora japonica L., one active constituents traditional Chinese medicine found to inhibit bioactivity cytokines (e.g., interleukin‐5) inflammatory responses, as well attenuate glucose lipid metabolism in related diseases. However, effects Sop cancer progression have not been systemically investigated. In this study, we performed comprehensive investigation Sop's function GBM using colony formation Transwell assays vitro, along subcutaneous xenograft tumor analysis vivo. We employed RNA sequencing bioinformatics conjunction Western blotting (WB) reverse transcription‐quantitative polymerase chain reaction (RT‐PCR) explore underlying mechanism. Our results demonstrated that suppressed U251 cell proliferation metastasis vitro inhibited tumorigenic behavior cells Further investigations revealed positive correlation between levels activated AMP‐activated protein kinase (AMPK) treatment; notably application AMPK inhibitor, compound C (CC), abolished inhibitory phenotype cells. These findings suggest potential treatment highlight opportunities for development new therapeutic strategies.

Language: Английский

Citations

0
Identification of key immune genes of drug-induced liver injury induced by tolvaptan based on bioinformatics DOI

Xiyun Yang,

Ming Yan, Zhihui Zhou

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Language: Английский

Citations

0
Sophoricoside ameliorates cerebral ischemia-reperfusion injury dependent on activating AMPK DOI
Zhaoshuo Li, Mi Zhang, Lixia Yang

et al.

European Journal of Pharmacology, Journal Year: 2024, Volume and Issue: 971, P. 176439 - 176439

Published: Feb. 23, 2024

Language: Английский

Citations

2
Sophoricoside, a genistein glycoside from Fructus Sophorae, promotes hair growth via activation of M4 muscarinic AChR in dermal papilla cells DOI
Gary Ka-Wing Yuen, Shengying Lin,

Tina Ting-Xia Dong

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 334, P. 118585 - 118585

Published: July 16, 2024

Language: Английский

Citations

2
Sophoricoside reduces inflammation in type II collagen-induced arthritis by downregulating NLRP3 signaling DOI Creative Commons
Youyang Liu, Yunlu Zhao, Qi Guo

et al.

Biochemistry and Biophysics Reports, Journal Year: 2024, Volume and Issue: 40, P. 101867 - 101867

Published: Nov. 12, 2024

Language: Английский

Citations

1
Alleviating Effect of the Salt-Out Extract of Styphnolobium japonicum Fruit on Photo-Aging in the Skin of Hairless Mice DOI
Seong-Min Kim, Singeun Kim, Woo Jin Lee

et al.

Food Supplements and Biomaterials for Health, Journal Year: 2023, Volume and Issue: 3(4)

Published: Jan. 1, 2023

Language: Английский

Citations

1
Chronic UVB exposure induces hepatic injury in mice: mechanistic insights from integrated multi-omics DOI
Ruixuan Geng,

Jingya Guo,

Yujie Lao

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 362, P. 124933 - 124933

Published: Sept. 10, 2024

Language: Английский

Citations

0
The Correlation Analysis Between m6A Methylation Modification and Ferroptosis Induced by Cigarette Smoke in Human Bronchial Epithelium DOI Creative Commons
Xixin Duan,

Tingting Hu,

Lijuan Xu

et al.

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(12)

Published: Dec. 1, 2024

ABSTRACT Background Chronic obstructive pulmonary disease (COPD), a prevalent respiratory condition, is characterized by long‐term airway inflammation, which can lead to remodeling and persistent airflow restriction. Exposure cigarette smoke known as major contributor COPD development. Research has confirmed that ferroptosis m6A modification are closely related various inflammatory‐related diseases. However, the correlation between methylation in not been confirmed. In this study, combined with bioinformatics analysis molecular biology methods we investigated how was correlated ferroptosis‐associated genes (SLC7A11 NQO‐1) induced 16HBES cells. Methods Two microarray datasets (GSE30063 GSE64614) were identify differentially expressed (DEGs) through application of techniques. A (CS)‐induced 16HBE cells model established. The ROS, GSH, MDA, total iron content detected relevant detection kits. expression levels associated modification‐related determined via reverse transcription‐quantitative polymerase chain reaction western blot. Results Overall, 529 DEGs identified above two databases. For patients, significant changes observed FAGs (GCLC, NQO‐1, SLC7A11) methylation‐related (FTO). negative also noted level linked gene vitro experiments results indicate SLC7A11 NQO‐1 significantly downregulated, FTO upregulated. addition, stimulation increased LPO, while reducing GSH Conclusion Our findings explored relationship COPD, screened out SLC7A11, may be critical pathogenesis COPD.

Language: Английский

Citations

0
The potential of type 2 cystatin in blood feeding and innate immunity of the tick Haemaphysalis doenitzi (Acari: Ixodidae) DOI Creative Commons

Songbo Zhang,

Zhihua Gao,

Yikui Wang

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 16, 2023

Abstract Ticks, which are obligate blood-sucking parasites, transmit a wide range of pathogens during their blood-feeding process. Enzymes and macromolecules that inhibit enzymes play crucial role in tick physiology. In the present study, genes encoding type 2 cystatin were cloned characterized from Haemaphysalis doenitzi , functions innate immunity blood feeding further assessed. Four genes, namely HDcyst-1 HDcyst-2 HDcyst-3 HDcyst-4 successfully tick. Their open reading frames vary length, with sizes 390, 426, 426 base pairs (bp), respectively. Proteinase inhibition assays indicated 0.2 µM displayed minimum 50% inhibitory activity against cathepsin B S. The qPCR results imply could pivotal hemophagia, whereas may be integral to regulation digestion. Our investigation unveiled adult ticks subjected LPS injection or exposed low-temperature stress exhibited elevated expression levels compared control group. RNA interference experiments demonstrated cystatins had significant impact on engorged weight, number eggs laid, hatching rate, mortality H. along an extension duration by days. These discoveries suggest involvement processes. Consequently, emerges as promising candidate for development novel immunosuppressive drugs future.

Language: Английский

Citations

0