Engineered Macrophage Membrane-Coated Nanoparticles Attenuate Calcium Oxalate Nephrocalcinosis-Induced Kidney Injury by Reducing Oxidative Stress and Pyroptosis

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Association between the fatty liver index, metabolic dysfunction-associated steatotic liver disease and the risk of kidney stones

Kidney & Blood Pressure Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 25

Published: Jan. 2, 2025

Introduction: This study aimed to investigate the potential association between fatty liver index (FLI), metabolic dysfunction-associated steatotic disease (MASLD), and risk of kidney stones using large-scale population-based data. Methods: employed a cross-sectional design, utilizing data from 2007-2018 National Health Nutrition Examination Survey (NHANES) database. A total 24,342 participants were enrolled in study, status was assessed by calculating FLI. MASLD diagnosed FLI conjunction with cardiometabolic criteria. Data on history obtained self-report. We logistic regression models analyze FLI, MASLD, stone constructed multivariable adjustment control for confounders. Furthermore, we used restricted cubic spline curve dose-response relationship conducted subgroup interaction analyses. Results: The study's results indicate strong correlation increasing quartiles notable rise prevalence stones. Specifically, developing 1.68 times higher among highest quartile compared those lowest. patients exhibited 1.35-fold increased non-MASLD. Subgroup analyses demonstrated that consistent across multiple subgroups. However, significant observed subgroups smoking status, physical activity level, hypertension (interaction P < 0.05). analysis did not yield statistically nonlinear risk. identify inflection point values Conclusion: an suggests these conditions may be pivotal factors Further investigation is required elucidate associations' underlying mechanisms develop efficacious interventions reduce Also, formulating personalized prevention treatment strategies different population paramount.

Language: Английский

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Corn Silk Polysaccharides Before and After Selenization Reduced Calcium Oxalate Crystal-Induced HK-2 Cells Pyroptosis by Inhibiting the NLRP3-GSDMD Signaling Pathway

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 3623 - 3638

Published: March 1, 2025

Objective: Pyroptosis is a new type of programmed cell death associated with many inflammatory diseases. Polysaccharides have anti-inflammatory effects. In this study, we investigated whether corn silk polysaccharides (DCSP) before and after selenization (Se-DCSP) can reduce the renal tubule pyroptosis induced by calcium oxalate crystals. Methods: HK-2 cells were exposed to monohydrate size 3 μm (COM-3μm) establish model. The degree damage was determined detecting viability, reactive oxygen species (ROS), lactate dehydrogenase (LDH) content. proportion quantitatively detected Caspase-1/PI double staining. expression levels NLRP3, GSDMD, IL-18, IL-1β confocal microscopy Western blot analyses. Results: DCSP Se-DCSP secretion factors IL-1β/18 related reducing oxidative stress, as well down-regulate Caspase-1, TNF-α, repair damaged cells, inhibit in cells. inhibitory effect selenized polysaccharide significantly enhanced compared that selenification. Conclusion: through NLRP3/Caspase-1/GSDMD/IL-1β/IL-18 signaling pathway risk kidney-stone formation. Keywords: pyroptosis, kidney stones, crystals,

Language: Английский

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Comparison of Endoplasmic Reticulum Stress and Pyroptosis Induced by Pathogenic Calcium Oxalate Monohydrate and Physiologic Calcium Oxalate Dihydrate Crystals in HK-2 Cells: Insights into Kidney Stone Formation

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2070 - 2070

Published: Dec. 15, 2024

Endoplasmic reticulum stress (ERS) can activate pyroptosis through CHOP and TXNIP; however, the correlation between this process formation of kidney stones has not been reported. The purpose is to investigate effects calcium oxalate monohydrate (COM) dihydrate (COD) on ERS in HK-2 cells explore mechanism stones. were injured by 3 μm COM COD. COD significantly upregulated expression levels GRP78, CHOP, TXNIP, pyroptosis-related proteins (NLRP3, caspase-1, GSDMD-N, IL-1β). Fluorescence colocalization revealed that induced inducing interaction TXNIP NLRP3. Both crystals induce cells. induces with NLRP3 upregulation then promotes pyroptosis, while only CHOP. cytotoxicity ability promote crystal adhesion aggregation are higher than COD, suggesting more dangerous for stone formation.

Language: Английский

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Species-level characterization of gut microbiota and their metabolic role in kidney stone formation using full-length 16S rRNA sequencing

Urolithiasis, Journal Year: 2024, Volume and Issue: 52(1)

Published: Aug. 10, 2024

Language: Английский

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Copper Chelation by Tetrathiomolybdate Alleviates Kidney Damage from Calcium Oxalate Stones by Inhibiting Cuproptosis and Oxidative Stress

Published: Jan. 1, 2024

Language: Английский

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