Integrins as Key Mediators of Metastasis

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 904 - 904

Published: Jan. 22, 2025

Metastasis is currently becoming a major clinical concern, due to its potential cause therapeutic resistance. Its development involves series of phases that describe the metastatic cascade: preparation pre-metastatic niche, epithelial–mesenchymal transition, dissemination, latency and colonization new tissue. In last few years, targets, such as integrins, are arising face this disease. Integrins transmembrane proteins found in every cell have key role cascade. They intervene adhesion intracellular signaling dependent on extracellular matrix cytokines microenvironment. case, integrins can initiate guide formation niche increase tumor migration survival. also take part vascularization process necessary sustain metastasis. This fact emphasizes importance inhibitory therapies capable interfering with function

Language: Английский

Rapid Isolation and Characterization of Exosomes through a Single-Step, Label-Free Protein Biomarker Analysis

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Exosomes, small extracellular vesicles with compositions reflecting their cell of origin, serve as sensitive and specific biomarkers for disease detection. We herein report a protocol rapid isolation characterization exosomes by single-step label-free protein biomarker analysis. Using simple centrifugation-filtration-concentration (CFC) method, are isolated enriched 50-fold from conditioned culture media. For analysis, unconjugated antibodies added directly to the exosome solution. The interaction between leads aggregation exosomes, subsequently, an average particle size increase assay This can be detected using dynamic light scattering correlated presence or absence on exosomes. In this study, three types, human embryonic kidney (HEK293) cells, genetically modified HEK-GFP GBM/NSC CD133+ cells were isolated. released HEK293, HEK-GFP, exhibited monodispersed distributions centered around 70, 66, 249 nm, respectively. Positive antibody binding surface proteins resulted in peak shift, increasing 25, 32, 148 respectively, while upon addition negative remained minimum. provides convenient platform design development diagnostic tests targeting

Language: Английский

Basement membranes in lung metastasis growth and progression

Matrix Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

The lung is a highly vascularized tissue that often harbors metastases from various extrathoracic malignancies. Lung parenchyma consists of complex network alveolar epithelial cells and microvessels, structured within an architecture defined by basement membranes. Consequently, understanding the role extracellular matrix (ECM) in growth essential to uncover biology this pathology developing targeted therapies. These membranes play critical progression metastases, influencing multiple stages metastatic cascade, acquisition aggressive phenotype intravasation, extravasation colonization secondary sites. This review examines biological composition membranes, focusing on their core components-collagens, fibronectin, laminin-and specific roles cancer progression. Additionally, we discuss function integrins as primary mediators cell adhesion signaling between tumor cells, matrix, well implications for lung. We also explore vascular co-option (VCO) form resistance linked vasculature. Finally, covers current clinical therapies targeting adhesion, remodeling, development, aiming improve precision effectiveness treatments against metastases.

Language: Английский