Hippophae Rhamnoides‐derived Phytomedicine Nano‐System Modulates Bax/Fas Pathways to Reduce Proliferation in Triple‐Negative Breast Cancer

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(29)

Published: Aug. 12, 2024

Triple-negative breast cancer (TNBC) is the most common primary tumor of with limited effectual drug availability. Therefore, aim study to develop an innovative phyto-nanomedicine (PNM) cure TNBC least genotoxicity. Hereinafter, sea buckthorn' extracted polyphenols (SBP), combine metformin (MET), are synthesized as a novel PNM evaluate its anti-tumor properties, effectiveness, and mechanism action in vitro vivo models. The SBP exhibits 16 new kinds that been reported earlier which regulated cell development, proliferation, programmed death (PCD) effectively. SBP-MET inhibits MDA-MB-231 (47%), MDA-MB-436 (46%), 4T1 (46%) proliferation but does not affect L929 normal murine development successfully induce PCD (73.19%) cells. Mechanistically, proteome expression profiling reveals upregulation proapoptotic Bax protein activation Fas signaling pathways convince downstream Daxx FADD proteins, further triggers Caspase-3 prompts apoptosis human cells by cleaving PARP-1 protein. Current findings establish highly biocompatible has significant potential inhibit growth (RCD) model, thereby opening arena for therapy.

Language: Английский

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Ginkgetin delays the progression of osteoarthritis by inhibiting the NF-κB and MAPK signaling pathways.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Background Osteoarthritis (OA) is considered an advancing chronic degenerative joint disease, leading to severe physical functional impairment of patients. Its development closely related increased inflammation and oxidative stress within the joint. Ginkgetin (GK), a natural non-toxic chemical, has proven anti-inflammatory, antioxidant, anti-tumor, neuroprotective effects.Methods First, this study utilizes network pharmacology explore intrinsic connection between GK OA. In vitro, SW1353 human cartilage cells were stimulated with Tert-butyl hydrogen peroxide (TBHP), different concentrations pre-treated evaluate its protective effects. GK's anti-inflammatory antioxidative effects comprehensively assessed via MTT assay, western blot, cell immunofluorescence, ELISA, transcriptome sequencing. Potential underlying mechanisms also explored. vivo, OA was induced in rats anterior cruciate ligament transection (ACLT), impact on protection further histological analysis blot.Results Network revealed that regulates several key pathways, especially NF-κB, HIF-1, PI3K-AKT, substances like reactive oxygen species. vitro experiments showed effectively reverses damage from TBHP, inhibits inflammatory factor release, protects cellular matrix (ECM) degradation. These functions may be achieved NF-κB MAPK signaling pathways. vivo significantly reduced proteoglycan loss ACLT inhibited metalloproteinase 13 (MMP13) glycan protease 5 (ADAMTS5) production, preventing degeneration rats.Conclusion The research findings indicate novel approach for treatment

Language: Английский

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Modification of Cotton with Chitosan: Deposition of Copper(II) Sulfate by Complexation Copper Ions

Processes, Journal Year: 2024, Volume and Issue: 12(12), P. 2772 - 2772

Published: Dec. 5, 2024

This study introduces a novel approach for enhancing the functional properties of cotton fibers through complexation copper sulfate, and subsequent combination with chitosan (COT-CuSO4-CTS). Our preliminary investigations focused on development composites as candidate materials coatings antimicrobial properties. The were thoroughly characterized via scanning electron microscopy (SEM) optical microscopy, providing insights into their structural features composition. findings show that modified exhibit potent activity. Specifically, COT-CuSO4 COT-CuSO4-CTS samples demonstrated zones inhibition against both Gram-positive Staphylococcus aureus Gram-negative Escherichia coli, confirming ability to reduce microbial growth significantly. incorporation layer significantly enhanced Ultraviolet Protection Factor (UPF) fabric from 3.37 over 50, indicating exceptional UV shielding capabilities, while copper(II) oxide treatment provided moderate UPF value 14.56. Blood compatibility studies further revealed fabrics influence coagulation parameters, marked prolongation in activated partial thromboplastin time (aPTT) prothrombin (PT) compared untreated cotton. anticoagulant effect is primarily linked presence copper, although addition modulates this response, slightly reducing clotting times alone. Cytotoxicity genotoxicity assessments using Peripheral Mononuclear (PBM) cells indicated was non-toxic non-genotoxic. However, displayed reduction cell viability induced DNA damage, highlighting potential cytotoxic genotoxic effects. Notably, showed lower cytotoxicity than COT-CuSO4-CTS, suggesting reduces overall composite. Furthermore, plasmid relaxation assays interact DNA, exhibiting stronger interaction consistent PBM cells.

Language: Английский

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