MBene Nanosheets with DNA Adsorbability for Circulating Tumor DNA Assay via Fluorescence Biosensing and Paper‐Based Microfluidic POCT DOI Open Access
Siyi Yang,

Zhu Jia-jia,

Liangyi Zhao

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Abstract The detection of circulating tumor DNA (ctDNA) in blood is significant for non‐invasive cancer diagnosis and treatment monitoring. Herein, MBene nanosheets synthesized compared with MXene via Density Functional Theory (DFT) calculations fluorescence kinetic evaluations, first time, revealing MBene's exceptional adsorbability discrimination to single‐stranded (ssDNA) double‐stranded (dsDNA). Then, a sensitive biosensor ctDNA developed, demonstrating impressive performance. To facilitate point‐of‐care testing (POCT) ctDNA, paper‐based microfluidic chip incorporated delay area mixing channel designed. constricted‐expanded structure optimization guided by numerical simulations experiments. A WeChat mini program named “ctDNA Detection” designed readout assay. Furthermore, cell mice serum samples are analyzed, Magnetic Bead@Graphene Oxide (MB@GO) clutch probes magnetic pre‐enrichment. results accuracy confirmed its consistency standard qPCR (AUC = 1). successful post‐surgery mouse models underscored the biosensor's potential Thus, this research not only advanced understanding MBene‐DNA interaction biosensing, but also can pave way novel applications bioimaging nanopore‐based nucleic acid sequencing, leveraging digital transformation base‐MBene adsorption differences.

Language: Английский

MBene Nanosheets with DNA Adsorbability for Circulating Tumor DNA Assay via Fluorescence Biosensing and Paper‐Based Microfluidic POCT DOI Open Access
Siyi Yang,

Zhu Jia-jia,

Liangyi Zhao

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Abstract The detection of circulating tumor DNA (ctDNA) in blood is significant for non‐invasive cancer diagnosis and treatment monitoring. Herein, MBene nanosheets synthesized compared with MXene via Density Functional Theory (DFT) calculations fluorescence kinetic evaluations, first time, revealing MBene's exceptional adsorbability discrimination to single‐stranded (ssDNA) double‐stranded (dsDNA). Then, a sensitive biosensor ctDNA developed, demonstrating impressive performance. To facilitate point‐of‐care testing (POCT) ctDNA, paper‐based microfluidic chip incorporated delay area mixing channel designed. constricted‐expanded structure optimization guided by numerical simulations experiments. A WeChat mini program named “ctDNA Detection” designed readout assay. Furthermore, cell mice serum samples are analyzed, Magnetic Bead@Graphene Oxide (MB@GO) clutch probes magnetic pre‐enrichment. results accuracy confirmed its consistency standard qPCR (AUC = 1). successful post‐surgery mouse models underscored the biosensor's potential Thus, this research not only advanced understanding MBene‐DNA interaction biosensing, but also can pave way novel applications bioimaging nanopore‐based nucleic acid sequencing, leveraging digital transformation base‐MBene adsorption differences.

Language: Английский

Citations

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