Upcycling of SARS-CoV-2 Rapid Antigen Test Cassettes into Flame Retardant Plastics DOI Open Access

Tadej Slatinek,

Janez Slapnik

Materials, Journal Year: 2024, Volume and Issue: 17(10), P. 2384 - 2384

Published: May 16, 2024

The COVID-19 pandemic resulted in the generation of large quantities medical waste and highlighted importance efficient management systems. One good example this is rapid antigen tests, which contain valuable resources, are usually incinerated after their use. present study aimed to evaluate potential test cassettes (RATCs) as a resource for preparation sustainable flame-retardant plastics. Milled RATCs were compounded with different concentrations (10-30 wt.%) aluminium diethylphosphinate (ADP) injection moulded into specimens. Prepared samples exposed ultraviolet (UV) ageing varying durations characterised by Fourier-transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), dynamic mechanical (DMA), tensile Charpy impact vertical burning tests. FT-IR revealed that composed mainly high-impact polystyrene (HIPS), was further confirmed suitable glass transition temperatures (Tg) determined DSC DMA. addition ADP progressive embrittlement HIPS increasing concentration, while flammability decreased significantly reached V-1 classification at loading 30 wt.%. UV caused photo-oxidative degradation HIPS, strain-at-break, not affected.

Language: Английский

Microfluidic immunoassays for point-of-care testing of SARS-CoV-2 antigens and antibodies DOI

Cuili Li,

Wan Zhou,

Angel Gutierrez Ruiz

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 177, P. 117809 - 117809

Published: June 6, 2024

Language: Английский

Citations

9
Recent Advances in Quantum Dot-Based Lateral Flow Immunoassays for the Rapid, Point-of-Care Diagnosis of COVID-19 DOI Creative Commons
Seyyed Mojtaba Mousavi, Masoomeh Yari Kalashgrani, Ahmad Gholami

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(8), P. 786 - 786

Published: Aug. 3, 2023

The COVID-19 pandemic has spurred demand for efficient and rapid diagnostic tools that can be deployed at point of care to quickly identify infected individuals. Existing detection methods are time consuming they lack sensitivity. Point-of-care testing (POCT) emerged as a promising alternative due its user-friendliness, rapidity, high specificity Such tests conveniently conducted the patient’s bedside. Immunodiagnostic offer identification positive cases urgently required. Quantum dots (QDs), known their multimodal properties, have shown potential in terms combating or inhibiting virus. When coupled with specific antibodies, QDs enable highly sensitive viral antigens patient samples. Conventional lateral flow immunoassays (LFAs) been widely used simplicity, low cost, portability. However, often sensitivity required accurately detect loads. dot (QD)-based alternative, offering significant advancements specificity. Moreover, immunoassay (LFIA) method, which fulfils POCT standards, gained popularity diagnosing COVID-19. This review focuses on recent QD-based LFIA diagnosis. Strategies enhance using explored, underlying principles elucidated. benefits method highlighted, published performance diagnostics is examined. Overall, integration quantum holds immense promise revolutionizing detection, treatment, prevention, convenient effective approach combat pandemic.

Language: Английский

Citations

12
Advanced techniques and nanotechnologies for point-of-care testing DOI Creative Commons
Valeria Secchi, Alice Armanni, Linda Barbieri

et al.

Frontiers in Nanotechnology, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 15, 2025

Hospitals and other healthcare facilities harbor a complex interplay of microbial pathogens. The correct understanding pathogens distribution evolution is therefore crucial for infections control the design effective prevention strategies. In parallel, integration cutting-edge nanotechnologies early detection monitoring these specific target considered most approach to face nosocomial infections. this context, point-of-care (POC) testing, also known as near-patient becoming increasingly important. review we provide systematic insight into recent scientific technological advances in pathogen that explore advanced realize devices nanobiosensors, with improved selectivity sensitivity. particular, report on diffused affordable developed still developing POC aim increase sensitivity, speed accuracy different environments, from intensive care units outside laboratory hospital settings. text sub-divided several sections, each one focused type nanomaterials techniques actually employed.

Language: Английский

Citations

0
Crystal Structures of Antigen-Binding Fragment of Anti-Osteocalcin Antibody KTM219 DOI Open Access

Shuma Yazaki,

M. Komatsu,

Jinhua Dong

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 648 - 648

Published: Jan. 14, 2025

Osteocalcin is a useful biomarker for bone formation and bone-related diseases. KTM219 an anti-osteocalcin C-terminal peptide antibody. The single-chain variable region (scFv) antigen-binding fragment (Fab) of are applicable to the Quenchbody (Q-body) immunoassay. Q-body new type fluorescent immunosensor, which scFv or Fab labeled with dye. When binds its antigen, fluorescence intensity increases. highly sensitive detection antigens by changes in performed single step mixing sample reagent. In this study, reveal recognition mechanism antibody discuss structural basis Q-body, we solved crystal structures complex antigen osteocalcin (BGP-C7). Also, structure grown presence dye, carboxytetramethylrhodamine (TAMRA); however, tightly bound TAMRA was not found electron density map. We predicted binding sites pocket docking simulations. These results support proposed mechanism. would be further development improvement immunosensors.

Language: Английский

Citations

0
SARS-CoV-2 neutralizing antibody determination after vaccination using spectrophotometric measurement of lateral flow immunochromatography DOI Creative Commons
Jianglei Ma,

Scott Kaniper,

Yuliya Vabishchevich

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 24, 2025

Neutralizing antibody titers have been found to be strongly correlated with observed vaccine effectiveness against symptomatic and severe COVID-19. Few non-high complexity assays are currently available detect the presence of neutralizing antibodies. This retrospective single-center cross-sectional study compared performance a lateral flow immunochromatography assay coupled spectrophotometric measurement system for detecting SARS-CoV-2 antibodies an enzyme-linked immunosorbent (ELISA) neutralization in context post-vaccination responses. The limit detection was similar ELISA strong linearity throughout measuring interval. Repeatability, interfering substances, cross-reactivity studies were robust. Results 274 plasma samples on whom RNA test vaccination status, including number manufacturer, known showed positive predictive value (PPV) 99.0% (CI 96.4–99.7%) negative (NPV) 91.9% 83.4–96.2%) ELISA. PPV all manufacturer subgroups > 95% except those individuals who had only 1 Pfizer (PPV 80%). NPV PCR no vaccinations 100% while 88.1% without previous or vaccination. 80% contrast Moderna vaccinations. Alternative methodologies requiring less sophisticated laboratory support measure may useful

Language: Английский

Citations

0
Formation and Detection of Gizzerosine in Animal Feed Matrices: Progress and Perspectives DOI

Di Jiao,

Fan Jiao,

Zhen‐Jie Qian

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(7), P. 3247 - 3258

Published: Feb. 6, 2024

Gizzerosine is responsible for gizzard erosion and black vomit, owing to excessive gastric acid secretion in poultry. It a biogenic amine that forms during feed processing. Gizzerosine, derivative of histamine, serious threat animal safety poultry production because it more potent after ingestion harmful than histamine. The difficulty obtaining gizzerosine the lack simple, rapid, sensitive vitro detection techniques have hindered studies on effects health production. In this review, we evaluated natural formation chemical synthesis methods introduced seven their principles analyzing gizzerosine. This review summarizes issues research suggests future development methods.

Language: Английский

Citations

2
COVID-19 point-of-care tests can identify low-antibody individuals: In-depth immunoanalysis of boosting benefits in a healthy cohort DOI Creative Commons
Michael L. Mallory, Jennifer E. Munt, Tara M. Narowski

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(24)

Published: June 12, 2024

The recommended COVID-19 booster vaccine uptake is low. At-home lateral flow assay (LFA) antigen tests are widely accepted for detecting infection during the pandemic. Here, we present feasibility and potential benefits of using LFA-based antibody as a means individuals to detect inadequate immunity make informed decisions about immunization. In health care provider cohort, investigated changes in breadth depth humoral T cell immune responses following mRNA vaccination boosting LFA-positive LFA-negative groups. We show that negative LFA closely reflect lack functional observed battery sophisticated assays, while positive results do not necessarily adequate immunity. After vaccination, both groups gain systemic antibodies against evolving SARS-CoV-2 related viruses. Our findings can alert COVID-19, thereby increasing shots promoting herd

Language: Английский

Citations

2
Advancements and future directions in cardiac biomarker detection using lateral flow assays DOI
Stella Schuster da Silva,

Mylena Lemes Cunha,

Lucas B. Ayres

et al.

Analytical Methods, Journal Year: 2023, Volume and Issue: 15(30), P. 3610 - 3630

Published: Jan. 1, 2023

Lateral flow assays (LFAs) have emerged as one of the most prominent paper-based biosensor platforms for rapidly detecting and quantifying analytes linked to cardiovascular health.

Language: Английский

Citations

5
Ultrasensitive Rapid Antigen Test by Geometric Lateral Flow Assays and Highly Doped Upconversion Nanoparticles DOI
Le Zhang, Shihui Wen, Jawairia Umar Khan

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(42), P. 16581 - 16589

Published: Oct. 7, 2024

The paper-based lateral flow assay (LFA) testing strips are currently the most widely used for point-of-care (POCT), valued their rapid result turnaround times in a few minutes. However, sensitivity has been limited. Upconversion nanoparticles (UCNPs), especially highly doped ones, have emerged as promising luminescent reporters to enhance LFA sensitivity. These UCNPs exhibit nonlinear enhancement luminescence with excitation power density, necessitating higher densities brightness. In this study, we utilized geometric paper strip design minimize immune reaction area and maximize enabling ultrasensitive detection of SARS-CoV-2 nucleoprotein antigen. This also slowed antigen on strip, extending time between antibody, thereby enhancing efficiency reaction. Through design, our approach achieved over 100-fold limit (LOD) compared LFAs, based gold colloidal europium nanoparticles. innovation expands scope applications that require low LOD.

Language: Английский

Citations

1
A semi-quantitative visual lateral flow immunoassay for SARS-CoV-2 antibody detection for the follow-up of immune response to vaccination or recovery DOI Creative Commons
Simone Cavalera, Fabio Di Nardo, Thea Serra

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(8), P. 2139 - 2149

Published: Jan. 1, 2024

A visual semiquantitative lateral flow immunoassay (sqLFIA) was developed for the detection of anti-SARS-CoV-2 antibodies promoted by vaccination or infection. The sqLFIA discriminates classes immune individuals numbering coloured lines.

Language: Английский

Citations

1