Multiple full-length homozygous IGH haplotypes from Mauritian cynomolgus macaques DOI Creative Commons

Simone Olubo,

William S. Gibson,

Trent M. Prall

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Background: Nonhuman primates are frequent experimental models for human disease pathology and vaccine design. However, the vast mostly uncatalogued immunogenomic diversity of typical species adds complexity to interpretation experiments hinders reproducibility. Mauritian cynomolgus macaques (MCM) offer a unique opportunity circumvent these difficulties, due their restricted genetic diversity. Results: We assembled high-quality immunoglobulin heavy chain (IGH) haplotypes from long-read genomic sequencing 13 MCM. Four animals were homozygous IGH, yielding 3 distinct haplotypes. IGH haplotype H1 was observed in two homozygotes 5 additional heterozygous animals, accounting half assemblies recovered. The exhibited considerable variation, including 125 kilobase region that duplicated twice H3. Furthermore, shares only 83% average sequence identity with locus rhesus macaque reference genome, addition numerous large structural variations. annotated IG gene content all complete MCM found 298 functional IGHV alleles, which 94 (32%) not existing databases. also identified 69 IGHD 11 IGHJ 38 constant alleles across isotypes. Conclusions: In total, we multiple common genetically diverse within provide annotations facilitate future work this important animal model.

Language: Английский

Multiple full-length homozygous IGH haplotypes from Mauritian cynomolgus macaques DOI Creative Commons

Simone Olubo,

William S. Gibson,

Trent M. Prall

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Background: Nonhuman primates are frequent experimental models for human disease pathology and vaccine design. However, the vast mostly uncatalogued immunogenomic diversity of typical species adds complexity to interpretation experiments hinders reproducibility. Mauritian cynomolgus macaques (MCM) offer a unique opportunity circumvent these difficulties, due their restricted genetic diversity. Results: We assembled high-quality immunoglobulin heavy chain (IGH) haplotypes from long-read genomic sequencing 13 MCM. Four animals were homozygous IGH, yielding 3 distinct haplotypes. IGH haplotype H1 was observed in two homozygotes 5 additional heterozygous animals, accounting half assemblies recovered. The exhibited considerable variation, including 125 kilobase region that duplicated twice H3. Furthermore, shares only 83% average sequence identity with locus rhesus macaque reference genome, addition numerous large structural variations. annotated IG gene content all complete MCM found 298 functional IGHV alleles, which 94 (32%) not existing databases. also identified 69 IGHD 11 IGHJ 38 constant alleles across isotypes. Conclusions: In total, we multiple common genetically diverse within provide annotations facilitate future work this important animal model.

Language: Английский

Citations

0