Circadian regulation of the lactate metabolic kinetics in mice using the [ 1 H- 13 C]-NMR technique DOI Creative Commons
Lili Chen, Kefan Wu, Jin‐Gang He

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 26, 2023

Abstract Lactate is not only the energy substrate of neural cells, but also an important signal molecule in brain. In modern societies, disturbed circadian rhythms pose a global challenge. Therefore, exploring influence period on lactate and its metabolic kinetics essential for advancement neuroscientific research. present study, mice were infused [3- 13 C] through tail vein duration two minutes. After 30 minutes metabolism, animals sacrificed brain tissue was extracted tested. And then [ 1 H- NMR technology employed to investigate kinetic information metabolism different regions during various time periods acute sleep deprivation, detect enrichment neurochemicals. The results revealed fluctuating concentrations throughout day, with lower levels light higher dark periods. Most metabolites displayed strong sensitivity rhythm, exhibiting significant day-night variations. Conversely, few showed changes after primarily temporal region. Interestingly, contrast liver exhibited increase following deprivation. This study explored hinted at potential clinical implications disorders involving rhythm disturbances, providing new research basis exploration metabolism.

Language: Английский

Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis DOI

Tian Feng,

Yunling Gao, Di Hu

et al.

Chinese Chemical Letters, Journal Year: 2023, Volume and Issue: 35(8), P. 109259 - 109259

Published: Oct. 29, 2023

Language: Английский

Citations

13
The role of TRPV4 in acute sleep deprivation-induced memory impairment: mechanisms of calcium dysregulation and synaptic plasticity disruption DOI Creative Commons

Meimei Guo,

Feiyang Zhang,

Sha Liu

et al.

Cell Insight, Journal Year: 2025, Volume and Issue: unknown, P. 100240 - 100240

Published: March 1, 2025

Acute sleep deprivation (ASD) impairs memory formation, but the underlying mechanisms remain unclear. In this study, we employed an ASD model combined with fear conditioning to investigate these mechanisms. mRNA sequencing revealed upregulated expression of Transient Receptor Potential Vanilloid 4 (TRPV4), a nonselective Ca2+-permeable cation channel critical for calcium signaling, in mice ASD-induced impairments. Notably, TRPV4 knockdown reversed deficits. was associated increased intracellular Ca2+ concentrations, reduced spine density, and decreased postsynaptic density protein 95 (PSD95), key regulator synaptic plasticity. These findings suggest that may cause overload, leading disrupted plasticity impaired learning memory. Importantly, significantly mitigated impairments, contributed restoration. Together, demonstrate protective role against deficits highlight as potential therapeutic target impairment ASD.

Language: Английский

Citations

0
AlkB-Facilitated Demethylation Enables Quantitative and Site-Specific Detection of Dual Methylation of Adenosine in RNA DOI

Xia Guo,

Neng‐Bin Xie,

Wei Chen

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 96(2), P. 847 - 855

Published: Dec. 30, 2023

RNA molecules undergo various chemical modifications that play critical roles in a wide range of biological processes. N6,N6-Dimethyladenosine (m6,6A) is conserved modification and essential for the processing rRNA. To gain deeper understanding functions m6,6A, site-specific accurate quantification this indispensable. In study, we developed an AlkB-facilitated demethylation (AD-m6,6A) method detection m6,6A RNA. The N6,N6-dimethyl groups can cause reverse transcription to stall at site, resulting truncated cDNA. However, found Escherichia coli AlkB demethylase effectively demethylate RNA, generating full-length cDNA from AlkB-treated By quantifying amount produced using quantitative real-time PCR, were able achieve Using AD-m6,6A method, successfully detected quantified position 1851 18S rRNA 937 mitochondrial 12S human cells. Additionally, level 1007 was significantly reduced lung tissues sleep-deprived mice compared with control mice. Overall, provides valuable tool easy, accurate, quantitative, which aid uncovering diseases.

Language: Английский

Citations

10
Sleep Disorders: Pathogenesis and Therapeutic Interventions DOI Creative Commons

Cheng Liu,

Zhigang He,

Yanqiong Wu

et al.

MedComm, Journal Year: 2025, Volume and Issue: 6(3)

Published: March 1, 2025

Sleep disorder significantly disrupts the quality of life for patients. Although it is clinically acknowledged, fundamental neuropathological mechanisms are still not understood. Recent preclinical research has been directed toward understanding underlying sleep deprivation and sleep/wake dysregulation. linked to changes in structure function neural basis cognition. We reviewed circuits related disorders, along with alterations connectivity brain region functions, based on advancements electrophysiology optogenetic/chemogenetic techniques. subsequently outline cellular molecular modifications disorders studies, primarily involving neuronal metabolism, electrophysiological activity, synaptic plasticity, glial cells. Correspondingly, crosstalk between peripheral organs, we elucidate involvement celiac disease hepatic pathogenesis disorders. In this review, mainly discussed at molecular, cellular, circuit levels that contribute disorder. The review also covered potential strategies treating future avenues.

Language: Английский

Citations

0
Ectopic expression of Slc1a2 in the prefrontal cortex of sleep-deprived male mice counteracts the glutamate/GABA-glutamine dysfunction DOI Creative Commons
Fengying Zhang, Li Yao, Li Jiang

et al.

BMC Biology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 26, 2025

Language: Английский

Citations

0
Astaxanthin Mitigates ADHD Symptoms in Spontaneously Hypertensive Rats via Dopaminergic Modulation and Brain–Gut Axis Regulation DOI Creative Commons
Yuxin Leng, Ning Wu, Jing Wang

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(7), P. 1637 - 1637

Published: April 7, 2025

Attention Deficit Hyperactivity Disorder (ADHD) is a prevalent neurodevelopmental disorder that significantly impacts learning, daily functioning, and personal development. Astaxanthin (ASTA), naturally occurring antioxidant, has garnered interest as potential therapeutic agent for various diseases, particularly in mitigating oxidative stress. This study explores novel application of ASTA the context ADHD, aiming to investigate its effects underlying mechanisms. Spontaneously hypertensive rats (SHRs), widely used ADHD model animals, were treated with (50/100 mg/kg/day) three weeks, 5 mg/kg/day atomoxetine (ATO) positive, Wistar Kyoto (WKY) control. Behavioral improvements assessed using open field test (OFT) Morris water maze (MWM). Biochemical analyses conducted evaluate changes levels neurotrophic factors, while histological examinations performed assess neuroprotective effects. Additionally, role brain–gut axis was investigated. The behavioral symptoms hyperactivity, anxiety, impaired spatial memory animals mitigated by ASTA. improvement primarily attributed restoration neurotransmitter levels, dopamine (DA), achieved through modulation several critical components within system, including receptor 1 (DR1), transporter (DAT), tyrosine hydroxylase (TH), synaptic-associated protein 25 (SNAP-25). regulating serotonin (SERT) glial cell-derived factor (GDNF) supports recovery facilitates optimal brain Furthermore, cerebellar cells protected, structure intestinal microbiota regulated. can mitigate SHR dopaminergic multiple neurotransmitters, neuro-intestinal environment, which establishes promising nutraceutical candidate adjunctive therapy pediatric ADHD.

Language: Английский

Citations

0
Circadian Regulation of the Lactate Metabolic Kinetics in Mice Using the [1H-13C]-NMR Technique DOI
Lili Chen, Kefan Wu, Jin‐Gang He

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(8), P. 5802 - 5813

Published: Jan. 17, 2024

Language: Английский

Citations

1
Sleep Disturbance as a Predictor of Accelerated Human Aging DOI Creative Commons
A. V. Gorodnicheva, О. П. Ковтун, T. O. Brodovskaya

et al.

Ural Medical Journal, Journal Year: 2024, Volume and Issue: 23(3), P. 146 - 162

Published: July 12, 2024

Introduction . One of the main predictors aging is sleep disturbance, study role which in development accelerated process recognized as an urgent problem for medical community. The aim work to analyze modern data on age-related aspects status and describe known markers early associated with somnological disorders. Materials methods search selection literary sources was carried out databases PubMed, eLibrary, CyberLeninka, Google Scholar. Search depth — 17 years, from 2007 2024. total number publications included review 80. Results discussion. Aging refers a general pathological that affects all human physiological systems. Differences between terms biological chronobiological age are established. disorders has been determined. It established increases, changes electrophysiological pattern observed. found positively correlated disturbance. importance neuroimaging determining rate people shown. Conclusion Sleep disturbance defined significant indicator body. Further research into structure quality may change understanding pathogenetic mechanisms contribute new diagnostic therapeutic strategies field anti-aging medicine.

Language: Английский

Citations

1
The role of gut microbiota in chronic restraint stress-induced cognitive deficits in mice DOI Creative Commons

Qiong Ling,

Junhong Zhang, Lin Zhong

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Aug. 2, 2024

Chronic stress induces cognitive deficits. There is a well-established connection between the enteric and central nervous systems through microbiota-gut-brain (MGB) axis. However, effects of gut microbiota on deficits remain unclear. The present study aimed to elucidate composition in explore its potential predicting chronic stress-induced Mice were randomly divided into control restraint (CRS) groups. mice subjected CRS further deficit (CRS-CD) non-cognitive (CRS-NCD) groups using hierarchical cluster analysis novel object recognition test results. diversity analyzed. After being distress, CRS-CD travelled shorter movement distances (p = 0.034 vs. CRS-NCD; p < 0.001 control) had lower index than CRS-NCD 0.0001 mice. results revealed that 5 bacteria at genus levels significantly different fecal samples three Further analyses demonstrated Muricomes not only enriched group but also correlated with decreased index. area under receiver operating curve for CRS-induced was 0.96. Our indicates involved development induced by stress. have predict

Language: Английский

Citations

1
Curcumin nanoparticles in heat stroke management DOI Creative Commons

Fei Guo,

Yizhan Wu,

Jiangwei Liu

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 12, 2024

Language: Английский

Citations

1