Selective Vulnerability to Neurodegenerative Disease: Insights from Cell Type-Specific Translatome Studies

Biology, Journal Year: 2024, Volume and Issue: 13(2), P. 67 - 67

Published: Jan. 23, 2024

Neurodegenerative diseases (NDs) manifest a wide variety of clinical symptoms depending on the affected brain regions. Gaining insights into why certain regions are resistant while others susceptible is vital for advancing therapeutic strategies. While gene expression changes offer clues about disease responses across regions, mixture cell types therein obscures experimental results. In recent years, methods that analyze transcriptomes individual cells (e.g., single-cell RNA sequencing or scRNAseq) have been widely used and provided invaluable specific types. Concurrently, transgene-based techniques dissect type-specific translatomes (CSTs) in model systems, like RiboTag bacTRAP, unique advantages but received less attention. This review juxtaposes merits drawbacks both methodologies, focusing use CSTs understanding conditions amyotrophic lateral sclerosis (ALS), Huntington’s (HD), Alzheimer’s (AD), prion fatal familial insomnia (FFI), genetic Creutzfeldt–Jakob (gCJD), acquired disease. We conclude by discussing emerging trends observed multiple methods.

Language: Английский

Overview of human transmissible spongiform encephalopathies

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 529 - 545

Published: Dec. 4, 2024

Language: Английский