Ascorbic acid alleviates reproductive toxicity of di-(2-ethyl hexyl) phthalate in female Wistar rats DOI Creative Commons
Kalaivani Manokaran, Ravi Sankar Bhaskaran, Jayesh Mudgal

et al.

Asian Pacific Journal of Reproduction, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Objective: To investigate the potential of ascorbic acid in mitigating reproductive toxicity induced by di-(2-ethyl hexyl) phthalate (DEHP) female Wistar rats, focusing on oxidative stress, hormone levels, and gonadotropin receptors expression. Methods: Forty rats [30 days old, weighing (60±10)g] were randomly divided into five groups ( n =8 per group). Group 1 received corn oil (control). Groups 2 3 administered DEHP at 10 100 mg/kg body weight (b.wt.), respectively. 4 5 mg/ kg b.wt., respectively, plus b.wt.. All treatments given orally for 30 days. Blood ovarian tissues collected to assess serum hormones, receptor gene expression, stress markers, apoptosis. Results: DEHP, particularly higher dose, significantly decreased levels (follicle-stimulating hormone, luteinizing estradiol) expression (FSHR, LHR), while increasing Co-treatment with improved these parameters, reducing apoptosis, restoring Histopathology revealed fewer atretic follicles less disruption structure acid-treated compared those treated alone. Conclusions: Ascorbic demonstrates protective effects against DEHP-induced likely through normalizing function.

Language: Английский

Ascorbic acid alleviates reproductive toxicity of di-(2-ethyl hexyl) phthalate in female Wistar rats DOI Creative Commons
Kalaivani Manokaran, Ravi Sankar Bhaskaran, Jayesh Mudgal

et al.

Asian Pacific Journal of Reproduction, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Objective: To investigate the potential of ascorbic acid in mitigating reproductive toxicity induced by di-(2-ethyl hexyl) phthalate (DEHP) female Wistar rats, focusing on oxidative stress, hormone levels, and gonadotropin receptors expression. Methods: Forty rats [30 days old, weighing (60±10)g] were randomly divided into five groups ( n =8 per group). Group 1 received corn oil (control). Groups 2 3 administered DEHP at 10 100 mg/kg body weight (b.wt.), respectively. 4 5 mg/ kg b.wt., respectively, plus b.wt.. All treatments given orally for 30 days. Blood ovarian tissues collected to assess serum hormones, receptor gene expression, stress markers, apoptosis. Results: DEHP, particularly higher dose, significantly decreased levels (follicle-stimulating hormone, luteinizing estradiol) expression (FSHR, LHR), while increasing Co-treatment with improved these parameters, reducing apoptosis, restoring Histopathology revealed fewer atretic follicles less disruption structure acid-treated compared those treated alone. Conclusions: Ascorbic demonstrates protective effects against DEHP-induced likely through normalizing function.

Language: Английский

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