Embryonically generated interneurons (EGINs) exhibit a distinctive developmental program and lamination in the olfactory bulb DOI Open Access

Natalie J. Spence,

Eduardo Martín-López, Kimberly Han

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 25, 2024

Interneurons (INs) in the mouse olfactory bulb (OB) develop during an extensive period of time that begins embryo and continues throughout lifetime individual. The connectivity location these neurons is profoundly affected by generation, making developmental windows critical to understand circuitry OB. Here, we focus on OB embryonic generated interneurons (or EGINs), which have generally received less attention than those adult. Birthdates EGINs were differentiated injections thymidine analogs their final destinations phenotypes analyzed immunohistochemistry. We found retained adult distributed across all layers However, a lateral-to-medial neurogenic gradient seen only earliest EGINs. Within granule cell layer (GCL), predominantly accumulated superficial region at almost ages remained there adulthood. Immunostaining for calbindin, parvalbumin, tyrosine hydroxylase, calretinin largely negative suggesting may represent subpopulations INs are not yet fully characterized. Using utero electroporation label EGIN progenitors, demonstrated they reach primordial as early E13 begin differentiate apical dendrites E15. At E16, progenitors clustered into chain migrating neuroblasts denoting rostral migratory stream (RMS). Collectively, our data highlight importance studying isolated better formation circuits define responses.

Language: Английский

Lateral Ventricular Neural Stem Cells Provide Negative Feedback to Circuit Activation Through GABAergic Signaling DOI Creative Commons
Moawiah M. Naffaa, Henry H. Yin

Cells, Journal Year: 2025, Volume and Issue: 14(6), P. 426 - 426

Published: March 13, 2025

Recent studies have demonstrated that circuit activation in vivo can regulate proliferation of lateral ventricular neural stem cells (LV NSCs), although the underlying molecular and cellular mechanisms are not yet fully understood. Here, we investigated role GABAergic signaling interaction between LV NSCs anterior cingulate cortex-subependymal-choline acetyltransferase+ (ChAT+) neuron (ACC-subep-ChAT+) circuit. We found monoamine oxidase B (MAOB), a key enzyme involved gamma-aminobutyric acid (GABA) synthesis, is expressed NSCs, ACC-subep-ChAT+ modulate MAOB activity. Additionally, express LRRC8D, core component volume-regulated anion channels, GABA transporter-1 (GAT-1, SLC6A1). show evidence that, through signaling, LRRC8D GAT-1 provide negative feedback signal to ChAT+ neurons, NSCs. These findings suggest MAOB-driven LRRC8D-regulated chloride transport, GAT-1-facilitated reuptake influence NSC dynamics LV.

Language: Английский

Citations

0
Olfactory bulb interneurons – The developmental timeline and targeting defined by embryonic neurogenesis DOI

Natalie J. Spence,

Eduardo Martín-López, Kimberly Han

et al.

Molecular and Cellular Neuroscience, Journal Year: 2025, Volume and Issue: unknown, P. 104007 - 104007

Published: March 1, 2025

Language: Английский

Citations

0
Embryonically generated interneurons (EGINs) exhibit a distinctive developmental program and lamination in the olfactory bulb DOI Open Access

Natalie J. Spence,

Eduardo Martín-López, Kimberly Han

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 25, 2024

Interneurons (INs) in the mouse olfactory bulb (OB) develop during an extensive period of time that begins embryo and continues throughout lifetime individual. The connectivity location these neurons is profoundly affected by generation, making developmental windows critical to understand circuitry OB. Here, we focus on OB embryonic generated interneurons (or EGINs), which have generally received less attention than those adult. Birthdates EGINs were differentiated injections thymidine analogs their final destinations phenotypes analyzed immunohistochemistry. We found retained adult distributed across all layers However, a lateral-to-medial neurogenic gradient seen only earliest EGINs. Within granule cell layer (GCL), predominantly accumulated superficial region at almost ages remained there adulthood. Immunostaining for calbindin, parvalbumin, tyrosine hydroxylase, calretinin largely negative suggesting may represent subpopulations INs are not yet fully characterized. Using utero electroporation label EGIN progenitors, demonstrated they reach primordial as early E13 begin differentiate apical dendrites E15. At E16, progenitors clustered into chain migrating neuroblasts denoting rostral migratory stream (RMS). Collectively, our data highlight importance studying isolated better formation circuits define responses.

Language: Английский

Citations

0