Cortistatin exerts an immunomodulatory and neuroprotective role in a preclinical model of ischemic stroke DOI Creative Commons

J Castillo-González,

Lara Buscemi, Pablo Vargas-Rodríguez

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 210, P. 107501 - 107501

Published: Nov. 7, 2024

Ischemic stroke is the result of a permanent or transient occlusion brain artery, leading to irreversible tissue injury and long-term sequelae. Despite ongoing advancements in revascularization techniques, remains second cause death worldwide. A comprehensive understanding complex interconnected mechanisms, along with endogenous mediators that modulate responses essential for development effective interventions. Our study investigates cortistatin, neuropeptide extensively distributed immune central nervous systems, known its immunomodulatory properties. With neuroinflammation peripheral deregulation as key pathological features ischemia, cortistatin emerges promising therapeutic candidate. To this aim, we evaluated potential effect well-established middle cerebral artery (MCAO) preclinical model. findings indicated administration at 24 h post-stroke significantly reduced neurological damage enhanced recovery. Importantly, cortistatin-induced neuroprotection was multitargeted, it modulated glial reactivity astrocytic scar formation, facilitated blood-brain barrier recovery, regulated local systemic dysfunction. Surprisingly, immediate early time points proved be not beneficial even detrimental. These results emphasize importance spatio-temporal dynamics pathology develop innovative strategies appropriate windows. Premature interruption certain neuroinflammatory processes might inadvertently compromise neuroprotective mechanisms. In summary, our highlights novel pleiotropic approach against ischemic stroke, offering new treatment options patients who undergo intervention but unsuccessful

Language: Английский

Responsive nanoparticles synergize with Curcumin to break the “reactive oxygen Species-Neuroinflammation” vicious cycle, enhancing traumatic brain injury outcomes DOI Creative Commons
Xianhua Fu, Yongkang Zhang, Guojie Chen

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 5, 2025

Traumatic brain injury (TBI) disrupts oxygen homeostasis in the brain, leading to excessive reactive species (ROS) production and dysregulated antioxidant mechanisms, which fail clear excess ROS. This ROS overload promotes expression of pro-inflammatory genes, releasing cytokines chemokines creating a vicious "ROS-neuroinflammation" cycle, making it essential break this cycle for effective TBI treatment. In study, we developed cysteine-alanine-glutamine-lysine (CAQK) peptide-modified nanoparticles (C-PPS/C) co-delivery curcumin (Cur) modulate oxidative neuroinflammatory disturbances after TBI. mice, C-PPS/C accumulated injured regions, where poly (propylene sulfide)120 scavenged ROS, reducing stress, while Cur release further suppressed inflammation. broke protecting blood-brain barrier (BBB), acute edema, promoting long-term neurological recovery. Further investigation showed that inhibited NF-κB pathway, gene mitigating inflammation, suggesting promising approach

Language: Английский

Citations

1
Towards advanced regenerative therapeutics to tackle cardio-cerebrovascular diseases DOI Creative Commons
Xi Chen,

Weiping Lin,

Micky D. Tortorella

et al.

American Heart Journal Plus Cardiology Research and Practice, Journal Year: 2025, Volume and Issue: 53, P. 100520 - 100520

Published: March 1, 2025

The development of vascularized organoids as novel modelling tools the human cardio-cerebrovascular system for preclinical research has become an essential platform studying tissues/organs personalized therapeutics during recent decades. Organ-on-chip technology is promising investigating physiological in vitro responses drug screening and advanced disease models. Vascularized tissue/organ-on-a-chip benefits every step discovery pipeline a tool with close genome relevance to investigate systems biology. Simultaneously, cardio-cerebrovascular-on-chip-integrated microfluidic serves alternative animal (patho-)physiological processes blood vessels embryonic disease. Integrated next-generation techniques, such three-dimensional bioprinting both cells matrix, may enable organoid-on-chip-based therapeutics.

Language: Английский

Citations

0
Research Progress on Pericytes in Central Nervous System Diseases DOI

浩 潘

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(04), P. 3357 - 3368

Published: Jan. 1, 2025

Language: Английский

Citations

0
The current approaches to modeling the brain ischemia–reperfusion and inflammation: from animal models toward vascularized and neuroimmune cerebral organoids DOI
Pavel P. Tregub,

Daria D. Volegova,

Arseniy K. Berdnikov

et al.

Reviews in the Neurosciences, Journal Year: 2025, Volume and Issue: unknown

Published: May 27, 2025

Abstract For several decades, the modeling of brain diseases in experimental animals has remained one key components studying pathogenesis central nervous system pathology and searching for new methods prevention therapy. In recent years, approaches to pathological conditions vitro have been active development; these will not only reduce number animal studies but also allow us take a step toward reproducing human-specific mechanisms pathology. this review, we characterize most common rodent models cerebral ischemia reperfusion, as well neuroinflammation inherent neurodegeneration (in particular, Parkinson’s disease), which are reproduced vivo . This review addresses engineering technical challenges prospects development , e.g., vascularized microglia-containing/neuroimmune organoids, may be useful overcoming shortcomings limitations current models.

Language: Английский

Citations

0
Enhancing Upper Limb Exoskeletons Using Sensor-Based Deep Learning Torque Prediction and PID Control DOI Creative Commons
Farshad Shakeriaski, Masoud Mohammadian

Sensors, Journal Year: 2025, Volume and Issue: 25(11), P. 3528 - 3528

Published: June 3, 2025

Upper limb assistive exoskeletons help stroke patients by assisting arm movement in impaired individuals. However, effective control of these systems to survivors is a complex task. In this paper, novel approach proposed enhance the upper using torque estimation and prediction proportional–integral–derivative (PID) controller loop more optimally integrate exoskeleton robot, which aims eliminate system uncertainties. First, model for from Electromyography (EMG) signals predictive robot elbow are trained. The trained data consisted two-dimensional high-density surface EMG (HD-sEMG) record myoelectric activity five muscles (biceps brachii, triceps anconeus, brachioradialis, pronator teres) during voluntary isometric contractions twelve healthy subjects performing four different tasks (supination/pronation flexion/extension) one minute each, were on long short-term memory (LSTM), bidirectional LSTM (BLSTM), gated recurrent units (GRU) deep neural network models. These models estimate predict requirements. Finally, estimated predicted used online as input PID dynamic, optimally. results showed that method creates strong innovative greater independence rehabilitation improvement.

Language: Английский

Citations

0
Cortistatin exerts an immunomodulatory and neuroprotective role in a preclinical model of ischemic stroke DOI Open Access
Julia Castillo-González, Lara Buscemi, Pablo Vargas-Rodríguez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 9, 2024

Abstract Ischemic stroke is the result of a permanent or transient occlusion brain artery, leading to irreversible tissue injury and long-term sequelae. Despite ongoing advancements in revascularization techniques, remains second cause death worldwide. A comprehensive understanding complex interconnected mechanisms, along with endogenous mediators that modulate responses essential for development effective interventions. Our study investigates cortistatin, neuropeptide extensively distributed immune central nervous systems, known its immunomodulatory properties. With neuroinflammation peripheral deregulation as key pathological features ischemia, cortistatin emerges promising therapeutic candidate. To this aim, we evaluated potential effect well-established middle cerebral artery (MCAO) preclinical model. findings indicate administration at 24 hours post-stroke significantly reduces neurological damage enhances recovery. Importantly, cortistatin-induced neuroprotection was multitargeted, it modulated glial reactivity astrocytic scar formation, facilitated blood-brain barrier recovery, regulated local systemic dysfunction. Surprisingly, immediate early time points proved be not beneficial even detrimental. These results emphasize importance spatio-temporal dynamics pathology develop innovative strategies. Premature interruption certain neuroinflammatory processes might inadvertently compromise neuroprotective mechanisms. In summary, our highlights novel pleiotropic approach against ischemic stroke, offering new treatment options patients whom intervention unsuccessful. Graphical abstract

Language: Английский

Citations

1
Cortistatin exerts an immunomodulatory and neuroprotective role in a preclinical model of ischemic stroke DOI Creative Commons

J Castillo-González,

Lara Buscemi, Pablo Vargas-Rodríguez

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 210, P. 107501 - 107501

Published: Nov. 7, 2024

Ischemic stroke is the result of a permanent or transient occlusion brain artery, leading to irreversible tissue injury and long-term sequelae. Despite ongoing advancements in revascularization techniques, remains second cause death worldwide. A comprehensive understanding complex interconnected mechanisms, along with endogenous mediators that modulate responses essential for development effective interventions. Our study investigates cortistatin, neuropeptide extensively distributed immune central nervous systems, known its immunomodulatory properties. With neuroinflammation peripheral deregulation as key pathological features ischemia, cortistatin emerges promising therapeutic candidate. To this aim, we evaluated potential effect well-established middle cerebral artery (MCAO) preclinical model. findings indicated administration at 24 h post-stroke significantly reduced neurological damage enhanced recovery. Importantly, cortistatin-induced neuroprotection was multitargeted, it modulated glial reactivity astrocytic scar formation, facilitated blood-brain barrier recovery, regulated local systemic dysfunction. Surprisingly, immediate early time points proved be not beneficial even detrimental. These results emphasize importance spatio-temporal dynamics pathology develop innovative strategies appropriate windows. Premature interruption certain neuroinflammatory processes might inadvertently compromise neuroprotective mechanisms. In summary, our highlights novel pleiotropic approach against ischemic stroke, offering new treatment options patients who undergo intervention but unsuccessful

Language: Английский

Citations

0