Molecular Detection of HPV E7 Gene Using TaqMan Probe-Based RT-PCR: A Case-Control Study in Hilla City, Iraq DOI Creative Commons

Mohammed Mohsen Mohammed Al-Ziara,

Zaytoon A. Al-Khafaji,

Nadia Mudher Al-Hilli

et al.

Al-Rafidain Journal of Medical Sciences ( ISSN 2789-3219 ), Journal Year: 2025, Volume and Issue: 8(1), P. 106 - 111

Published: Feb. 18, 2025

Background: Human papillomavirus (HPV) is a key driver of cervical cancer, with high-risk genotypes, mainly HPV 16 and 18, responsible for approximately 70% cases. Persistent infections immunological evasion by increase the risk malignant transformation. Objective: To evaluate effect TaqMan probes in real-time PCR pivotal role DNA detection, targeting highly conserved regions like E7 gene. Methods: A case-control study was conducted on 71 women Hilla City, Iraq, to detect DNA. The used swabs blood samples, Statistical analysis, including chi-square tests, evaluated associations between demographic, clinical, behavioral variables. Results: Out 22 tested positive HPV, primarily using (95.5%). Amplification gene showed high efficiency (100.9%) specificity. Significant were found infection factors such as smoking, rural residence, family history cancers. No co-infections observed. Conclusions: This underscores effectiveness probe-based RT-PCR detection highlights critical demographic factors. findings advocate expanding vaccination diagnostic access, particularly underserved regions.

Language: Английский

Molecular Detection of HPV E7 Gene Using TaqMan Probe-Based RT-PCR: A Case-Control Study in Hilla City, Iraq DOI Creative Commons

Mohammed Mohsen Mohammed Al-Ziara,

Zaytoon A. Al-Khafaji,

Nadia Mudher Al-Hilli

et al.

Al-Rafidain Journal of Medical Sciences ( ISSN 2789-3219 ), Journal Year: 2025, Volume and Issue: 8(1), P. 106 - 111

Published: Feb. 18, 2025

Background: Human papillomavirus (HPV) is a key driver of cervical cancer, with high-risk genotypes, mainly HPV 16 and 18, responsible for approximately 70% cases. Persistent infections immunological evasion by increase the risk malignant transformation. Objective: To evaluate effect TaqMan probes in real-time PCR pivotal role DNA detection, targeting highly conserved regions like E7 gene. Methods: A case-control study was conducted on 71 women Hilla City, Iraq, to detect DNA. The used swabs blood samples, Statistical analysis, including chi-square tests, evaluated associations between demographic, clinical, behavioral variables. Results: Out 22 tested positive HPV, primarily using (95.5%). Amplification gene showed high efficiency (100.9%) specificity. Significant were found infection factors such as smoking, rural residence, family history cancers. No co-infections observed. Conclusions: This underscores effectiveness probe-based RT-PCR detection highlights critical demographic factors. findings advocate expanding vaccination diagnostic access, particularly underserved regions.

Language: Английский

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