Deciphering the role of metal ion transport-related genes in T2D pathogenesis and immune cell infiltration via scRNA-seq and machine learning

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 24, 2025

Introduction Type 2 diabetes (T2D) is a complex metabolic disorder with significant global health implications. Understanding the molecular mechanisms underlying T2D crucial for developing effective therapeutic strategies. This study employs single-cell RNA sequencing (scRNA-seq) and machine learning to explore pathogenesis of T2D, particular focus on immune cell infiltration. Methods We analyzed scRNA-seq data from islet cells nondiabetic (ND) patients, identifying differentially expressed genes (DEGs), especially those related metal ion transport (RMITRGs). employed 12 algorithms develop predictive models assessed infiltration using single-sample gene set enrichment analysis (ssGSEA). Correlations between key RMITRGs were investigated, interactions among these explored through protein-protein interaction (PPI) network analysis. Additionally, we performed detailed cell-cell communication identify signaling pathways in T2D. Results Our identified 1953 DEGs ND Stepglm[backward] plus GBM model demonstrating high accuracy 13 hub RMITRGs. Twelve protein structures predicted AlphaFold 3, revealing potential functional conformations. observed strong correlation cells, PPI revealed interactions. Cell-cell highlighted 16 active pathways, CXCL, MIF, COMPLEMENT linked inflammatory responses, WNT, KIT, LIFR, HGF uniquely activated Conclusion emphasizing transport, signaling, These findings suggest enhance management. The provide valuable insights into disease targets intervention.

Language: Английский

Applications and prospects of biomaterials in diabetes management

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: March 7, 2025

Diabetes is a widespread metabolic disorder that presents considerable challenges in its management. Recent advancements biomaterial research have shed light on innovative approaches for the treatment of diabetes. This review examines role biomaterials diabetes diagnosis and treatment, as well their application managing diabetic wounds. By evaluating recent developments alongside future obstacles, highlights promising potential care, underscoring importance enhancing patient outcomes refining methodologies.

Language: Английский

Diabetic Cardiomyopathy: Role of Cell Death, Exosomes, Fibrosis and Epicardial Adipose Tissue

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9481 - 9481

Published: Aug. 31, 2024

Diabetic cardiomyopathy (DCM) represents one of the typical complications associated with diabetes. It has been described as anomalies in heart function and structure, consequent high morbidity mortality. DCM development can be by two stages; first is characterized left ventricular hypertrophy diastolic dysfunction, second failure (HF) systolic dysfunction. The proposed mechanisms involve cardiac inflammation, advanced glycation end products (AGEs) angiotensin II. Furthermore, different studies have focused their attention on cardiomyocyte death through programmed cell death, such apoptosis, autophagy, necrosis, pyroptosis ferroptosis. Exosome release, adipose epicardial tissue aquaporins affect development. This review will focus description involved progression

Language: Английский