Let-7b-5p sensitizes breast cancer cells to doxorubicin through Aurora Kinase B

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0307420 - e0307420

Published: Jan. 9, 2025

MicroRNAs (miRNAs) are small, non-coding RNAs that regulate the expression level of target genes in cell. Breast cancer is responsible for majority cancer-related deaths among women globally. It has been proven deregulated miRNAs may play an essential role progression breast cancer. shown many cancers, including cancer, aberrant be associated with drug resistance. This study investigated effect let-7b-5p, detected by bioinformatics methods, on Dox resistance through Aurora Kinase B ( AURKB ) gene. In silico analysis using publicly available miRNA expression, GEO datasets revealed let-7b-5p significantly downregulated BC. Further studies potential targets was most negatively correlated and closely Expression via quantitative PCR confirmed upregulated tissue samples. Later, functional conducted MCF-10A, MCF-7, MDA-MB-231 cell lines demonstrated inhibits cells sensitizes them to conclusion, it let-7b-5p/ axis significant disruption this contribute trigger

Language: Английский

Elevated expression of let-7b-3p enhances aggressiveness of larynx squamous cell carcinoma cells

Anatolian Current Medical Journal, Journal Year: 2025, Volume and Issue: 7(1), P. 27 - 32

Published: Jan. 10, 2025

Aims: Larynx squamous cell carcinoma (LSCC) is the second most common head and neck malignancy. While let-7b-3p has been shown to have a role in cancer progression malignancies, there no research examining association between LSCC let-7b-3p. This study aimed investigate expression status of potential roles this microRNA (miRNA) LSCC. Methods: Using quantitative real-time polymerase chain reaction (qRT-PCR), we examined let-7b3p 36 samples neighboring normal tissues. Then, miRNA mimic was transfected into Hep-2 cells via lipofectamine 2000 reagents. Cell viability determined using detection (CVDK-8) kit, migration evaluated with scratch assay. To identify differentially expressed genes (DEGs) larynx GSE137308 GSE130605 datasets were downloaded reanalyzed Gene Expression Omnibus (GEO2R) tool. Potential target investigated prediction functional annotation database (miRDB). Shared geo miRDB results identified relationship these literature. Results: We demonstrated that levels significantly upregulated tumor tissues comparison corresponding Mimic transfection enhanced proliferation migration. In vitro bioinformatics analysis showed overexpression can enhance through MYBPC1. Conclusion: It let-7b-3p/MYBPC1 axis could potentially affect process. Let-7b-3p be biomarker for LSCC, therefore, let-7b-3p/ MYBPC1/LSCC should elucidated new studies.

Language: Английский