Two draft genomes of enigmatic Solenogastres (Mollusca, Aplacophora) Epimenia babai and Neomenia megatrapezata DOI Creative Commons
Meghan K. Yap-Chiongco, S Pirro, Rebecca Varney

et al.

F1000Research, Journal Year: 2024, Volume and Issue: 13, P. 318 - 318

Published: April 23, 2024

Abstract* Many molluscan genomes have been published to date, however only three are from representatives of the subphylum Aculifera (Polyplacophora, Caudofoveata, and Solenogastres), sister taxon all other molluscs. Currently, genomic resources completely lacking for Solenogastres. This gap in knowledge hinders comparative evolutionary studies. Here, we sequenced solenogaster aplacophorans Epimenia babai Salvini-Plawen, 1997 Neomenia megatrapezata Salvini-Plawen & Paar-Gausch, 2004 using a hybrid approach combining Oxford Nanopore Illumina reads. For E. babai, produced 628 Mbp haploid assembly (N50 = 413 Kbp, L50 370) that is rather complete with BUSCO completeness score 90.1% (82.0% single, 8.1% duplicated, 6.0% fragmented, 3.9% missing). N. megatrapezata, 412 132 881) also 85.1% (81.7% 3.4% 6.8% Our annotation pipeline predicted 25,393 gene models 92.4% (80.5% 11.9% 4.9% 2.7% missing) 22,463 90.2% (81.0% 9.2% 4.7% 5.1% Phylogenomic analysis recovered Solenogastres as Polyplacophora sampled molluscs maximal support. These represent first whole-genome will be valuable future studies investigating this understudied group evolution whole.

Language: Английский

A genome-based phylogeny for Mollusca is concordant with fossils and morphology DOI Open Access
Zeyuan Chen, J. Antonio Baeza, Chong Chen

et al.

Science, Journal Year: 2025, Volume and Issue: 387(6737), P. 1001 - 1007

Published: Feb. 27, 2025

Extreme morphological disparity within Mollusca has long confounded efforts to reconstruct a stable backbone phylogeny for the phylum. Familiar molluscan groups—gastropods, bivalves, and cephalopods—each represent diverse radiation with myriad morphological, ecological, behavioral adaptations. The phylum further encompasses many more unfamiliar experiments in animal body-plan evolution. In this work, we reconstructed living on basis of metazoan BUSCO (Benchmarking Universal Single-Copy Orthologs) genes extracted from 77 (13 new) genomes, including multiple members all eight classes two high-quality genome assemblies monoplacophorans. Our analyses confirm proposed morphology show widespread genomic variation. flexibility likely explains both historic challenges their genomes evolutionary success.

Language: Английский

Citations

4
Still waters run deep in large-scale genome rearrangements of morphologically conservative Polyplacophora DOI Creative Commons
Julia D. Sigwart, Yunlong Li, Zeyuan Chen

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: April 17, 2025

A major question in animal evolution is how genotypic and phenotypic changes are related, another when whether ancient gene order conserved living clades. Chitons, the molluscan class Polyplacophora, retain a body plan general morphology apparently little changed since Palaeozoic. We present comparative analysis of five reference quality genomes, including four de novo assemblies, covering all chiton clades, an updated phylogeny for phylum. constructed 20 linkage groups (MLGs) show that these relatively bivalve karyotypes, but chitons they subject to re-ordering, rearrangement, fusion, or partial duplication vary even between congeneric species. The largest number novel fusions most plesiomorphic clade Lepidopleurida, chitonid Liolophura japonica has genome duplication, extending occurrence large-scale within Mollusca. extreme dynamic rearrangements this stands contrast other animals, demonstrating have overcome evolutionary constraints acting on groups. conservative phenome belies rapid extensive genome.

Language: Английский

Citations

2
Still waters run deep: Large scale genome rearrangements in the evolution of morphologically conservative Polyplacophora DOI Open Access
Julia D. Sigwart, Yunlong Li, Zeyuan Chen

et al.

Published: April 8, 2025

A major question in animal evolution is how genotypic and phenotypic changes are related, whether ancient gene order conserved living clades. Chitons, the molluscan class Polyplacophora, retain a body plan general morphology apparently little changed since Palaeozoic. We present comparative analysis of five reference quality genomes, including four de novo assemblies, covering all chiton clades, an updated phylogeny for phylum.We constructed 20 linkage groups (MLGs) that relatively bivalve karyotypes, but subject to re-ordering, rearrangement, fusion, or partial duplication among chitons, varying even between congeneric species. The largest number novel fusions most plesiomorphic clade Lepidopleurida, chitonid Liolophura japonica has genome duplication, extending occurrence large-scale within Mollusca.The extreme dynamic rearrangements this stands contrast other animals, demonstrating chitons have overcome evolutionary constraints acting on groups. conservative phenome belies rapid extensive genome.

Language: Английский

Citations

0
Two draft genomes of enigmatic Solenogastres (Mollusca, Aplacophora) Epimenia babai and Neomenia megatrapezata DOI Creative Commons
Meghan K. Yap-Chiongco, S Pirro, Rebecca Varney

et al.

F1000Research, Journal Year: 2024, Volume and Issue: 13, P. 318 - 318

Published: April 23, 2024

Abstract* Many molluscan genomes have been published to date, however only three are from representatives of the subphylum Aculifera (Polyplacophora, Caudofoveata, and Solenogastres), sister taxon all other molluscs. Currently, genomic resources completely lacking for Solenogastres. This gap in knowledge hinders comparative evolutionary studies. Here, we sequenced solenogaster aplacophorans Epimenia babai Salvini-Plawen, 1997 Neomenia megatrapezata Salvini-Plawen & Paar-Gausch, 2004 using a hybrid approach combining Oxford Nanopore Illumina reads. For E. babai, produced 628 Mbp haploid assembly (N50 = 413 Kbp, L50 370) that is rather complete with BUSCO completeness score 90.1% (82.0% single, 8.1% duplicated, 6.0% fragmented, 3.9% missing). N. megatrapezata, 412 132 881) also 85.1% (81.7% 3.4% 6.8% Our annotation pipeline predicted 25,393 gene models 92.4% (80.5% 11.9% 4.9% 2.7% missing) 22,463 90.2% (81.0% 9.2% 4.7% 5.1% Phylogenomic analysis recovered Solenogastres as Polyplacophora sampled molluscs maximal support. These represent first whole-genome will be valuable future studies investigating this understudied group evolution whole.

Language: Английский

Citations

1