Maternal undernutrition inhibits fetal rumen development： Novel miRNA-736-mediated dual targeting of E2F2 and MYBL2 in sheep DOI
Peng Jiao, Yun Xu,

Yun‐Qiong Gu

et al.

Published: May 13, 2025

Abstract Background Undernutrition disrupts pregnant ewe’s metabolic homeostasis and severely inhibits fetal growth development. In this study, undernourished nutrition-recovery sheep models rumen epithelial cells were utilized to investigate the mechanisms behind undernutrition-induced disruptions in metabolism Results Maternal undernutrition significantly reduced weight papilla length, width surface area. extremely suppressed nutrient energy production via JAK3/STAT3 signaling inhibit cell cycle progression development, while maternal nutritional recovery partially restored inhibition but failed alleviate Meanwhile, 64 differentially expressed miRNAs (DEMs) identified between ewes controls. Novel miR-736 was overexpressed both of models. E2F transcription factor 2 (E2F2) MYB proto-oncogene like (MYBL2) intersection genes (DEGs) DEMs target integrated analysis predicted as genes. Further, we confirmed that targeted downregulated E2F2 MYBL2 expressional levels. Silencing promoted apoptosis inhibited S-phase entry cells. Conclusions summary, disrupted elevated miR-736, which promote apoptosis, finally This study provides new insights into epigenetic underlying developmental deficits.

Language: Английский

Maternal undernutrition inhibits fetal rumen development： Novel miRNA-736-mediated dual targeting of E2F2 and MYBL2 in sheep DOI
Peng Jiao, Yun Xu,

Yun‐Qiong Gu

et al.

Published: May 13, 2025

Abstract Background Undernutrition disrupts pregnant ewe’s metabolic homeostasis and severely inhibits fetal growth development. In this study, undernourished nutrition-recovery sheep models rumen epithelial cells were utilized to investigate the mechanisms behind undernutrition-induced disruptions in metabolism Results Maternal undernutrition significantly reduced weight papilla length, width surface area. extremely suppressed nutrient energy production via JAK3/STAT3 signaling inhibit cell cycle progression development, while maternal nutritional recovery partially restored inhibition but failed alleviate Meanwhile, 64 differentially expressed miRNAs (DEMs) identified between ewes controls. Novel miR-736 was overexpressed both of models. E2F transcription factor 2 (E2F2) MYB proto-oncogene like (MYBL2) intersection genes (DEGs) DEMs target integrated analysis predicted as genes. Further, we confirmed that targeted downregulated E2F2 MYBL2 expressional levels. Silencing promoted apoptosis inhibited S-phase entry cells. Conclusions summary, disrupted elevated miR-736, which promote apoptosis, finally This study provides new insights into epigenetic underlying developmental deficits.

Language: Английский

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