CsNYC1a Mediates Chlorophyll Degradation and Albino Trait Formation in the Arbor-Type Tea Plant Camellia nanchuanica

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(24), P. 14087 - 14098

Published: June 7, 2024

Albino germplasms are prized tea plant mutants with yellow/white leaves. However, understanding of the albino mechanisms in non-Camellia sinensis species remains limited. This study elucidated trait formation Nanchuan Dachashu (C. nanchuanica), an arbor-type species, and its association quality. The yellow-leaved individual NH1 exhibited abnormal chloroplast ultrastructure reduced chlorophyll/carotenoid levels compared to green-leaved NL1. Integrating transcriptomics, metabolomics, yeast one-hybrid, transgenic approaches identified chlorophyll b reductase gene CsNYC1a as a key regulator, which was significantly up-regulated NH1, overexpression Arabidopsis recapitulated phenotype. In yeast, histone CsH1.2 binds promoter. These findings suggest that CsH1.2-CsNYC1a-mediated degradation may be mechanism underlying Dachashu. addition, germplasm higher polyphenol-to-amino acid ratio than NL1, offers more possibilities for breeding application.

Language: Английский

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Biosynthesis and Extraction of Chlorophyll, Carotenoids, Anthocyanins, and Betalaine In Vivo and In Vitro

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(9), P. 10662 - 10676

Published: Sept. 23, 2024

As natural bioactive compounds, plant pigments play crucial roles not only in phenotype, growth, development, and adaptation to stress but also hold unique value biotechnology, healthcare, industrial applications. There is growing interest the biosynthesis acquisition of pigments. Thus, this paper explores emerging extraction methods elucidates pathways four key pigments, chlorophylls, carotenoids, anthocyanins, betalaine vivo vitro. We comprehensively discuss application solvent, supercritical fluid [extraction], ultrasonic, microwave-assisted techniques, as well introducing enzymes, precursors, synthetic involved pigment synthesis. δ-Aminolevulinic acid represents a pivotal initiating enzyme for chlorophyll synthesis, whereas isopentenylpyrophosphate, (IPP) dimethylallyl pyrophosphate, (DMAPP) are closely associated with carotenoid biosynthesis. Phenylalanine tyrosine critical substances anthocyanin respectively. Hence, genes such chlI, crtB, PGT8, CYP76AD1, BvDODA can be employed heterologous vitro meet demand increased amount. determinant coloration, an in-depth exploration into high-quality provide basis developing superior offer new insights increasing yield.

Language: Английский

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Effects of Brassinosteroid on the Physiological Changes on Two Varieties of Tea Plants Under Salt Stress

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13445 - 13445

Published: Dec. 15, 2024

Salt stress is one of the abiotic stresses affecting crop quality and yield, application exogenous brassinosteroids (BRs) can be used in response to salt stress. However, function BR tea plants under remains elucidated. This study investigated effects spraying on malondialdehyde, soluble sugar, protein, antioxidant enzyme activities explored expression changes genes related synthesis pathways proline secondary metabolites (flavonoids theanine). The results show that 200 mM NaCl solution inhibits physiology plants, but 0.2 mg/L could partially reduce damage by increasing photosynthetic pigments, osmoregulatory substances (such as proline), activity enzymes (including peroxidase, catalase, superoxide dismutase), while decreasing malondialdehyde content salt-stressed leaves. qRT-PCR experiment also shows theanine) were upregulated stress, degradation downregulated, thus promoting accumulation both varieties. When subjected was regulated accordingly resist Moreover, had an obvious effect improving tolerance plants. Therefore, exploring a way improve trees provides reference for subsequent its mechanism, which great significance expanding introduction area trees, planting yield tea.

Language: Английский

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High-Throughput Transcriptomic Analysis of Circadian Rhythm of Chlorophyll Metabolism under Different Photoperiods in Tea Plants

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9270 - 9270

Published: Aug. 27, 2024

Tea plants are a perennial crop with significant economic value. Chlorophyll, key factor in tea leaf color and photosynthetic efficiency, is affected by the photoperiod usually exhibits diurnal seasonal variations. In this study, high-throughput transcriptomic analysis was used to study chlorophyll metabolism, under different photoperiods, of plants. We conducted time-series sampling skeleton (6L6D) continuous light conditions (24 L), measuring carotenoid content at interval 3 h h). Transcriptome sequencing performed six time points across two cycles, followed bioinformatics identify annotate differentially expressed genes (DEGs) involved metabolism. The results revealed distinct expression patterns biosynthetic pathway. levels

Language: Английский

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Developmental-specific regulation promotes the free amino acids accumulation in chlorotic tea plants (Camellia sinensis)

Journal of Plant Physiology, Journal Year: 2024, Volume and Issue: 303, P. 154371 - 154371

Published: Oct. 14, 2024

Language: Английский

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