Role of Antioxidative Peptide Gly‐Met Derived From Goat Milk Dadih Toward Antiaging Effects Through the SKN‐1/Nrf2 Pathway in C. elegans DOI Creative Commons
Wieda Nurwidada Haritsah Zain, Issei Yokoyama, Irdha Mirdhayati

et al.

Journal of Food Biochemistry, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

Fermented dairy products have been studied for their physiological functions, particularly antioxidant properties. Dadih is a traditional Indonesian fermented product produced from buffalo milk and prepared in bamboo tubes that exhibit functional Compared to cow milk, goat has gained more attention dadih production due its low allergen count ease of digestion. In our previous study, we identified the dipeptide glycine‐methionine (Gly‐Met) generated as novel peptide. This study aimed investigate antiaging effects Gly‐Met Caenorhabditis elegans using an vivo model. Initially, effective concentration (EC 50 ) was determined exhibited activity value 0.96 mg/mL. Our results demonstrated significantly extended mean lifespan, enhanced resistance oxidative stress, reduced reactive oxygen species (ROS) accumulation C. . Furthermore, mRNA expression Nsy-1 , Sek-1 Pmk-1 are target genes p38 MAPK signaling pathway upregulated C Subsequently, Skn-1 Gcs-1 levels were upregulated. Activation SKN‐1 may be modulated by pathway. revealed Gly‐Met, antioxidative peptide dadih, contribute through SKN‐1/Nrf2

Language: Английский

Ginsenoside Rh4 inhibits colorectal cancer via the modulation of gut microbiota-mediated bile acid metabolism DOI Creative Commons
Xue Bai, Zhiguang Duan, Jianjun Deng

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Dysbiosis of the gut microbiota is emerging as a pivotal factor in pathogenesis colorectal cancer (CRC). Ginsenoside Rh4 (Rh4) an active compound isolated from ginseng with beneficial effects modulating intestinal inflammation and dysbiosis, but how regulates to alleviate CRC remains underexplored. We investigated impact on mechanism its action inhibiting via modulation microbiota. used AOM/DSS model employed transcriptomics, genomics metabolomics techniques explore inhibitory CRC. Furthermore, we experiments involving antibiotic treatment fecal transplantation (FMT) investigate role Finally, elucidated key functional bacteria metabolites regulated by Our research findings indicated that repaired barrier damage caused CRC, alleviated inflammation, inhibited development Additionally, microbiota-dependent manner. increased diversity microbiota, enriched probiotic Akkermansia muciniphila (A. muciniphila), dysbiosis Subsequently, A. muciniphila-mediated bile acid metabolism. promoted production UDCA enhancing activity 7α-hydroxysteroid dehydrogenase (7α-HSDH). further activated FXR, modulated TLR4-NF-κB signaling pathway, thus results confirm inhibits manner microbiota-mediated metabolism promoting UDCA, which activates FXR receptor pathway. has potential be modulator for preventing

Language: Английский

Citations

8
Effect of Saccharomyces cerevisiae CICC 32883 Fermentation on the Structural Features and Antioxidant Protection Effect of Chinese Yam Polysaccharide DOI Creative Commons
Zichao Wang,

Yi Zheng,

Ziru Lai

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(4), P. 564 - 564

Published: Feb. 8, 2025

In this study, Chinese yam polysaccharide (CYP) was fermented by Saccharomyces cerevisiae CICC 32883, and its structural features antioxidant activities before after fermentation were analyzed. S. 32883-fermented CYP (CYP-SC) had higher carbohydrate content lower protein than the nonfermented (CYP-NF). The monosaccharide composition of CYP-SC unaffected, but proportion changed. Compared with CYP-NF's molecular weight polydispersity 124.774 kDa 6.58, respectively, those reduced to 20.384 3.379. Antioxidant results showed that better effects CYP-NF in scavenging DPPH, ABTS, hydroxyl, superoxide radicals. Moreover, enhancing oxidation capacity protecting HepG2 cells CYP-NF. Furthermore, on alleviating repairing H2O2-damaged are superior This work offers a green efficient method for activity dietary plant polysaccharides.

Language: Английский

Citations

1
Comprehensive review of plant-derived anti-hyperlipidemia peptides: Production, anti-hyperlipidemia mechanism, and structure-activity relationship study DOI
Liuyi Wei, Hao Wu, Xufeng Wang

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 461, P. 140715 - 140715

Published: Aug. 2, 2024

Language: Английский

Citations

4
Peptide Design for Enhanced Anti-Melanogenesis: Optimizing Molecular Weight, Polarity, and Cyclization DOI Creative Commons
Sheila Amara Putri, Rani Maharani,

Iman Permana Maksum

et al.

Drug Design Development and Therapy, Journal Year: 2025, Volume and Issue: Volume 19, P. 645 - 670

Published: Jan. 1, 2025

Melanogenesis is a biochemical process that regulates skin pigmentation, which crucial role in protecting against ultraviolet radiation. It also associated with hyperpigmentation conditions such as melasma and age spots, negatively impact aesthetics self-confidence. Tyrosinase (TYR), key enzyme the melanogenesis pathway, catalyzes biosynthesis of melanin skin. Inhibition tyrosinase particularly by blocking its active site preventing binding natural substrates tyrosine, can reduce production, making it promising therapeutic target for treating hyperpigmentation. Peptides have emerged therapeutics to regulate minimizing side effects conventional whitening therapeutics. This review designed offer comprehensive analysis current strategies peptide design aimed at optimizing anti-melanogenic activity, focusing on molecular weight, polarity, cyclization enhancing efficacy stability. was found optimal size within range 400-600 Da. The balance between hydrophilic hydrophobic properties peptides effective TYR inhibition, higher hydrophilicity enhances affinity stronger catalytic while hydrophobicity contribute through alternative mechanisms. Cyclization their structural stability, serum resistance, reducing toxicity. increases resistance enzymatic degradation improves specificity limiting conformational flexibility. Additionally, rigidity internal hydrogen bonding cyclic aid membrane permeability, them more use. Peptide optimizations modification, polarity change, been shown be reliable safe agents inhibition. Future studies exploring specific amino acid chains are required improve potential clinical applications these treatment.

Language: Английский

Citations

0
The effects of <i>Lactobacillus</i> fermentation on the quality changes and flavor characteristics of <i>Aronia melanocarpa</i> juice using physicochemical analysis and electronic nose techniques DOI Creative Commons
Yinan Wu, Rui Chen,

Minjun Liu

et al.

Food Innovation and Advances, Journal Year: 2025, Volume and Issue: 4(1), P. 99 - 107

Published: Jan. 1, 2025

Language: Английский

Citations

0
Effect of sheep placenta extract on D-galactose-induced aging mouse DOI Creative Commons
Shan He, Yuebin Wu, Kaifeng Lu

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 26, 2025

Introduction Sheep placenta extract (SPE) is a representative traditional medicinal substance that exhibits multiple experimentally validated physiological properties, including anti-aging effects, wound healing acceleration, antioxidant activity, and anti-inflammatory mechanisms. However, the mechanism by which SPE influences delay of aging still not yet clear. Methods Exploring effects sheep on D-gal induced senescence in mouse model macrogenomics metabolomics. Results In serum mice treated with SPE, levels function such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) were notably higher compared to those blank group, whereas malondialdehyde (MDA) decreased. We revealed alleviated changes gut microbiota caused mice, significant decrease Firmicutes/Bacteroidetes (F/B) ratio gut. Furthermore, Akkermansia muciniphila ( A. ), known for its regulating immune response potential showed increase 1177.94%. The analysis UHPLC-QE-MS combined orthogonal partial least squares discriminant (OPLS-DA) screening differential metabolites metabolic profiles upregulation cis -5,8,11,14,17-eicosapentaenoic acid (EPA) triptolide metabolites, following treatment, are commonly believed have immunosuppressive, anti-inflammatory, anti-proliferative, anti-tumor effects. Discussion role ameliorating may be associated increased abundance accumulation two EPA triptolide, serum.

Language: Английский

Citations

0
Phytochemicals and Health Benefits of Sweet Orange Fruit and Juice: A Comparative Summary and Reflection on Last Decade of Research DOI
Kewen Wang, Fuqing Gao, Fukai Li

et al.

Food Reviews International, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 26

Published: April 8, 2025

Language: Английский

Citations

0
Decreasing the pH value would improve the swallowing characteristics of whey protein isolate-enriched thickened fluids for dysphagia populations DOI
Zihang Cheng,

Kedu Wang,

Dongling Qiao

et al.

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116435 - 116435

Published: April 1, 2025

Language: Английский

Citations

0
Structural characterization of a low-molecular weight linear O-acetyl-glucomannan in Lilium lancifolium from Tibet and its protected H2O2-induced oxidative stress in HUVEC cells DOI Creative Commons
Lei Yuan,

Zheng-Chang Zhong,

Yu Liu

et al.

Chemical and Biological Technologies in Agriculture, Journal Year: 2024, Volume and Issue: 11(1)

Published: Jan. 25, 2024

Abstract In recent years, the biological activity of plant polysaccharides has received increasing attention. Polysaccharides, as one main components lilies, have pharmacological effects in regulating immunity, anti-tumor, antioxidant, antibacterial, and hypoglycemic effects. To systematically analyze structural characteristics polysaccharide, polysaccharide LP-1 was prepared from Lilium lancifolium bulbs by water extraction ethanol precipitation, ion exchange chromatography gel filtration chromatography. Structural characterizations show that weight-average relative molecular weight is 5.3 kDa. consists mannose glucose at a molar ratio 1.4:1. Its primary structure Glc p -α-D-(1 → {4)-β-D-Glc -(1 4)-β-D-2- O -acetyl-Man [4)-β-D-Glc 4)-β-D-Glc 4)-β-D-Man -(1] 2 } 4)-α-D-Glc p. Acetylation occurs -2 site mannose. Acetyl substitution degree 8.21%. The results elucidation showed low-molecular linear -acetylated glucomannan. oxidative stress experiments indicate exerts antioxidant on HUVEC activating Nrf2/HO-1 signaling pathway, thereby improving endothelial cell damage induced H , potential to be developed an drug. Graphical

Language: Английский

Citations

2
Facile Preparation of Magnetic COF-on-COF for Rapid Adsorption and Determination of Sulforaphane from Cruciferous Vegetables DOI Creative Commons
Jie Zhou,

Dan Xu,

Jiayong Cao

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(3), P. 409 - 409

Published: Jan. 26, 2024

Sulforaphane (SFN) is a natural isothiocyanate compound widely abundant in cruciferous vegetables with multiple bioactive functions. However, traditional analytical methods for the extraction and determination of SFN are cumbersome, time-consuming, low sensitivity large amounts organic solvents. Herein, novel magnetic COF-on-COFs (MB-COFs) were fabricated using Fe3O4 as core COFs-1 grown COFs-2 shell, they used efficient adsorbents dispersive solid-phase rapid quantification by combining HPLC-MS/MS. At optimal ratio to COFs-2, MB-COFs had spherical cluster-like structure rough surface, sufficient response separation (1 min). Due introduction exhibited outstanding efficiencies (92.5–97.3%), which was about 18–72% higher than that bare COFs. Moreover, showed good adsorption capacity (Qm 18.0 mg/g), (5 min) desorption (30 s) SFN, favorable reusability (≥7 cycles) virtue their unique hierarchical porous structure. The kinetic data well fitted pseudo-second-order, Ritchie-second-order, intra-particle diffusion, Elovich models, while isotherm highly consistent Langmuir, Temkin, Redlich–Peterson models. Finally, under optimized conditions, developed method wide linear range (0.001–0.5 mg/L), high (limits 0.18–0.31 μg/L), satisfactory recoveries (82.2–96.2%) precisions (1.8–7.9%), negligible matrix effect (0.82–0.97). Compared previous methods, proposed faster more sensitive significantly reduces use solvents, can achieve detection large-scale samples practical scenarios. This work reveals potential analysis vegetables.

Language: Английский

Citations

2