The complete mitochondrial genome and phylogenetic analysis of Lotus corniculatus (Fabaceae, Papilionoideae) DOI Creative Commons

Xiaofei Chen,

Zinian Wu,

Yanting Yang

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 11, 2025

Introduction Lotus corniculatus is a perennial leguminous herb and serves as high-quality forage, playing key role in both grassland ecological restoration the development of grazing livestock farming. Methods In this study, we successfully assembled L. mitochondrial genome investigated various related aspects, including genomic features, RNA editing sites, codon preference, gene transfer events, phylogeny. Results discussion We found that length 401,301 bp, its GC content 45.15%. It consists 53 genes, comprising 32 protein-coding 3 ribosomal 18 genes. A total 146 scattered repeats, 8 tandem 124 simple sequence repeats are present genome. thorough examination all genes revealed 485 instances 9579 codons. Additionally, 57 homologous fragments were identified chloroplast genomes, accounting for approximately 4.04% Furthermore, phylogenetic tree based on data from 33 species belonging to four Fabaceae subfamilies two other families validated evolutionary relationship Lotus. These findings have significant implications understanding organization evolution well identification genetic markers. They also offer valuable perspectives relevant devising strategies molecular breeding categorization legumes.

Language: Английский

Organelle genomes of two Scaevola species, S. taccada and S. hainanensis, provide new insights into evolutionary divergence between Scaevola and its related species DOI Creative Commons

Danni Meng,

Tianxin Lu,

Meng He

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 24, 2025

Chloroplast and mitochondrial genomes harbor crucial information that can be utilized for elucidating plant evolution environmental adaptation. The organellar genomic characteristics of Goodeniaceae, a sister family to Asteraceae, remain unexplored. Here, using combination short-read long-read sequencing technologies, we successfully assembled the complete two Goodeniaceae species native China, Scaevola taccada S. hainanensis. genome collinearity analysis revealed expanded its length through inverted repeat expansion large single copy fragment duplication, resulting in 181,022 bp (S. taccada) 182,726 hainanensis), ~30 kb increase compared related species. Mitochondrial exhibit multi-ring topology, forming dual chromosomes 314,251 276,175 hainanensis). Sequence variation demonstrated substantial chloroplast sequence divergence (Pi = 0.45) an gene number within genus. Relative synonymous codon usage (RSCU) has higher bias A/U-ending codons than mitochondria, with chloroplasts RSCU values ranging from 0.32 1.94, whereas 0.38 1.62. Phylogenetic analyses support monophyly Asteraceae-Goodeniaceae group, extended evolutionary branches Scaevola, coupled analysis, indicate rapid Scaevola. Organellar-nuclear horizontal transfer identified specific increased numbers photosynthesis-related genes chloroplast-nuclear events taccada. Our study not only provides insights understanding adaptation mechanisms coastal plants, but also contributes Goodeniaceae.

Language: Английский

Citations

0
Analysis of the complete mitogenomes of three high economic value tea plants (Tea-oil Camellia) provide insights into evolution and phylogeny relationship DOI Creative Commons
Heng Liang, Huasha Qi, Chunmei Wang

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 24, 2025

Tea-oil Camellia species play a crucial economic and ecological role worldwide, yet their mitochondrial genomes remain largely unexplored. In this study, we assembled analyzed the complete of oleifera C. meiocarpa, revealing multi-branch structures that deviate from typical circular genome observed in most plants. The mitogenomes span 953,690 bp (C. oleifera) 923,117 meiocarpa), containing 74 76 annotated genes, respectively. Comparative genomic analyses indicated meiocarpa share closer genetic relationship, whereas drupifera is more distantly related. Codon usage analysis revealed natural selection plays dominant shaping codon bias these genomes. Additionally, extensive gene transfer events were detected among three species, highlighting dynamic nature evolution Camellia. Phylogenetic reconstruction based on genes exhibited incongruence with chloroplast phylogenies, suggesting potential discordance due to hybridization events, incomplete lineage sorting (ILS), or horizontal (HGT). Furthermore, identified species-specific markers, which provide valuable molecular tools for distinguishing species. Our findings enhance understanding diversity Camellia, offering essential resources phylogenetics, identification, evolutionary research woody

Language: Английский

Citations

0
Complete mitochondrial genome of Medicago sativa ssp. falcata (Papilionoideae, Fabaceae): characterization and phylogenetic analysis DOI

Taiyou Ou,

Zinian Wu, Qian Liu

et al.

Planta, Journal Year: 2025, Volume and Issue: 261(6)

Published: April 29, 2025

Language: Английский

Citations

0
De novo assembly and comparative analysis of the first complete mitogenome in Distylium (Distylium racemosum) DOI Creative Commons

Yaling Wang,

Zhongxiao Zhang,

Xinru Chen

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: May 15, 2025

The genus Distylium (Hamamelidaceae) is highly valued for its applications in ornamental horticulture, industry, and construction. Although plastid genomes (plastomes) of multiple species have been characterized, no mitochondrial (mitogenomes) reported this genus. In study, we assembled annotated the complete mitogenome racemosum using HiFi sequencing data. comprises a longer circular chromosome shorter linear (904,264 bp total length), revealing structurally complex conformation. We 67 genes, including 43 protein-coding genes (PCGs), 21 tRNA three rRNA genes. Analyses identified exceptionally high repetitive sequence content, with 304 simple repeats, 1,508 dispersed 50 tandem representing highest repeat content among Saxifragales mitogenomes to date. Additionally, 49 DNA sequences were detected, only one plastid-derived gene ( trnC-GCA ) transferred mitogenome. predicted 697 RNA editing sites across 42 PCGs, further underscoring genome’s dynamic evolution. Phylogenetic reconstruction based on plastomes from 18 indicated D. occupied basal position within Saxifragales, which consistent APG IV classification system. This study provides first comprehensive mitogenomic resource genus, offering valuable insights molecular classification, identification, germplasm conservation plants.

Language: Английский

Citations

0
The complete mitochondrial genome and phylogenetic analysis of Lotus corniculatus (Fabaceae, Papilionoideae) DOI Creative Commons

Xiaofei Chen,

Zinian Wu,

Yanting Yang

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 11, 2025

Introduction Lotus corniculatus is a perennial leguminous herb and serves as high-quality forage, playing key role in both grassland ecological restoration the development of grazing livestock farming. Methods In this study, we successfully assembled L. mitochondrial genome investigated various related aspects, including genomic features, RNA editing sites, codon preference, gene transfer events, phylogeny. Results discussion We found that length 401,301 bp, its GC content 45.15%. It consists 53 genes, comprising 32 protein-coding 3 ribosomal 18 genes. A total 146 scattered repeats, 8 tandem 124 simple sequence repeats are present genome. thorough examination all genes revealed 485 instances 9579 codons. Additionally, 57 homologous fragments were identified chloroplast genomes, accounting for approximately 4.04% Furthermore, phylogenetic tree based on data from 33 species belonging to four Fabaceae subfamilies two other families validated evolutionary relationship Lotus. These findings have significant implications understanding organization evolution well identification genetic markers. They also offer valuable perspectives relevant devising strategies molecular breeding categorization legumes.

Language: Английский

Citations

0