Continuous Tracking for Effective Tackling: Ad5/35 Platform‐Based JN1 Lineage Vaccines Development in Response to Evolving SARS‐CoV‐2 Variants

Journal of Medical Virology, Journal Year: 2025, Volume and Issue: 97(2)

Published: Feb. 1, 2025

ABSTRACT The SARS‐CoV‐2 virus is continuously evolving, such that JN.1 and its subvariants, including KP.2, KP.3, LB.1, are now predominant variants globally. derived from BA.2.86, which harbors more than 30 mutations in the spike protein compared with those of XBB BA.2, it carries an additional L455S mutation. Given rapid evolution these variants, assessing neutralization capacity current lineage vaccines against prevalent as critical. Phylogenetic trees using sequences antigenic cartography based on results reveal antigenically distant previously circulating variants. Moreover, subvariants showed inadequate titers other XBB.1.5‐containing vaccine mice. Immunization targeting JN.1, LB.1 demonstrated significant neutralizing activity These highlight importance development to keep pace need for updated variant.

Language: Английский

Comparative study of anti-SARS-CoV-2 receptor-binding domain total antibody titer before and after heterologous booster with mRNA-based COVID-19 vaccine

Narra J, Journal Year: 2024, Volume and Issue: 4(3), P. e788 - e788

Published: Dec. 20, 2024

The waning immunity following the COVID-19 vaccination become a significant concern and immunological dynamics of vaccine-induced antibodies after need to be explored. aim this study was compare anti-SARS-CoV-2 receptor-binding domain (RBD) antibody levels before booster dose with heterologous vaccine identify factors influencing receiving dose. A cross-sectional conducted in which individuals who received primary doses CoronaVac an mRNA-based were recruited using purposive sampling technique. titers RBD measured enzyme-linked immunosorbent assay (ELISA), plausible associated collected questionnaire-assisted face-to-face interview. Wilcoxon test used dose, while Kruskal-Wallis Mann-Whitney tests, followed by multivariate linear regression, assess total titers. results showed that there increase (1,558.7 BAU/mL vs 140.6 BAU/mL, p<0.001). analysis revealed age (p=0.555), sex (p=0.254), type (p=0.914), presence hypertension (p=0.541), diabetes (p=0.975), chronic obstructive pulmonary disease (COPD, p=0.620), gout (p=0.364) not However, significantly different between those without hyperlipidemia (p=0.021). This suggests could enhance immune responses against COVID-19, therefore, strategy may recommended as part preventive measures strengthen COVID-19.

Language: Английский

Comparison of inflammatory mediator cytokine responses to inactivated virus platform COVID-19 vaccines between elderly and young adult populations.

PubMed, Journal Year: 2024, Volume and Issue: 4(3), P. e1380 - e1380

Published: Dec. 1, 2024

The coronavirus disease 2019 (COVID-19) pandemic has encouraged global vaccine research, yet effectiveness in the elderly remains a concern due to immunosenescence. aim of this study was compare cytokine response elicited by an inactivated virus-based COVID-19 between and young adults, focusing on key cytokines involved cellular humoral immunity: tumor necrosis factor-alpha (TNF-α), interleukin (IL)-2, IL-6, IL-10, interferon-gamma (IFN-γ). A cross-sectional conducted Jakarta-Bogor region Indonesia from January 2023 December 2023. population divided into two age cohorts: (60-85 years) younger adults (30-40 years). Blood samples were collected twice, after first booster dose four weeks second dose. Serum concentrations measured using Luminex assays with microparticles conjugated monoclonal antibodies against TNF-α, IL-2, IFN-γ. Comparisons levels Student's t-tests or Mann- Whitney U tests as appropriate. total 74 individuals included, 37 each adult groups. results showed significant differences responses After booster, IL-6 IFN- γ significantly higher compared elderly. still group (p = 0.001). Data indicated that dose, TNF-α increased only 0.004), while IL-2 0.040) IFN-γ 0.006) IL-10 both groups (both had p 0.020). This highlights stronger pro-inflammatory responses, relied more for T-cell immunity, suggesting need vaccination strategies optimize immune responses.

Language: Английский