Diet and skin health: The good and the bad

Nutrition, Journal Year: 2024, Volume and Issue: 119, P. 112350 - 112350

Published: Jan. 2, 2024

Language: Английский

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Exploring the Prospective Role of Propolis in Modifying Aging Hallmarks

Cells, Journal Year: 2024, Volume and Issue: 13(5), P. 390 - 390

Published: Feb. 24, 2024

Aging populations worldwide are placing age-related diseases at the forefront of research agenda. The therapeutic potential natural substances, especially propolis and its components, has led to these products being promising agents for alleviating several cellular molecular-level changes associated with diseases. With this in mind, scientists have introduced a contextual framework guide future aging research, called hallmarks aging. This encompasses various mechanisms including genomic instability, epigenetic changes, mitochondrial dysfunction, inflammation, impaired nutrient sensing, altered intercellular communication. Propolis, rich array bioactive compounds, functions as potent functional food, modulating metabolism, gut microbiota, immune response, offering significant health benefits. Studies emphasize propolis' properties, such antitumor, cardioprotective, neuroprotective effects, well ability mitigate oxidative stress, DNA damage, pathogenic bacteria growth. article underscores current scientific evidence supporting role controlling molecular characteristics linked hallmarks, hypothesizing geroscience research. aim is discover novel strategies improve quality life older individuals, addressing existing deficits perspectives area.

Language: Английский

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Research progress on the role of reactive oxygen species in the initiation, development and treatment of breast cancer

Progress in Biophysics and Molecular Biology, Journal Year: 2024, Volume and Issue: 188, P. 1 - 18

Published: Feb. 20, 2024

Language: Английский

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Thymoquinone Enhances Doxorubicin Efficacy via RAS/RAF Pathway Modulation in Ovarian Adenocarcinoma

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 536 - 536

Published: April 19, 2025

Background/Objectives: Ovarian cancer remains one of the most commonly diagnosed malignancies among women worldwide. The heterogeneity tumor subtypes and emergence treatment resistance have raised significant concerns regarding long-term efficacy chemotherapy, radiotherapy, immunotherapy. In response to these challenges, drug repurposing strategies—utilizing existing drugs in novel therapeutic contexts—have gained increasing attention. This study aimed investigate cytotoxic apoptotic effects combined application doxorubicin (DX) thymoquinone (TQ) on ovarian adenocarcinoma cells (OVCAR3). Methods: OVCAR3 were cultured RPMI medium supplemented with 10% fetal bovine serum (FBS) 1% penicillin/streptomycin. Cell viability proliferation assessed using MTT assay following various concentrations DX TQ. NucBlue immunofluorescence staining was employed examine nuclear morphology identify apoptosis-associated changes. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR) per-formed evaluate expression levels apoptosis-related oncogenic pathway genes, including RAF, RAS, Bcl-2, Bax. Results: results demonstrated that combination TQ significantly reduced cell induced apoptosis a dose-dependent manner. qRT-PCR analysis revealed downregulation Bcl-2 expression, along an upregulation Bax, indicating activation intrinsic pathway. These findings suggest exerts an-ti-proliferative pro-apoptotic by modulating RAS/RAF signaling cascade. Furthermore, co-administration potentiated effects, suggesting synergistic interaction between two agents. Conclusions: Histopathological molecular evaluations further confirmed suppression key pathways. Collectively, underscore potential as both monotherapy adjuvant conventional warranting validation preclinical clinical studies.

Language: Английский

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Apoptotic and Antiproliferative Effect of Thymoquinone on Ovarian Adenocarcinoma Cell Line Via The RAS/RAF Signaling Pathway

Published: July 30, 2024

Aim: Ovarian cancer is one of the most frequently diagnosed cancers in women world. Recognition differences between tumors and resistance to treatment patients have made potential chemotherapy, radiotherapy immunotherapy controversial. Especially against drug resistance, laboratories implemented diversion system by putting old drugs into new forms. In this study, cytotoxic apoptotic effects combination doxorubicin (DX) thymoquinone (TQ) on ovarian adenocarcinoma cells (OVCAR3) were investigated. Material Method: OVCAR3 cell line was cultured RPMI medium containing 10% FBS, 1% penicillin/streptomycin. Cell viability growth rate determined MTT test combining thymoquinone. Nucblue immunofluorescent staining used determine mechanism death at different doses compounds, qRT-PCR pathways underlying mechanism, western blot methods show protein levels. Result: We found that DX TQ reduced (MTT assay), induced apoptosis (RAF, RAS, Bcl2, Bax qRT-PCR) increased RAF, RAS levels DX-treated analysis. Conclusion: The results obtained revealed for first time also had cytotoxic/apoptotic anti-proliferative cells.

Language: Английский

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Antimicrobial, Anti-biofilm, Anti-swarming, Anti-quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye

Kastamonu University Journal of Forestry Faculty, Journal Year: 2024, Volume and Issue: 24(2), P. 158 - 167

Published: May 8, 2024

Aim of study: Studies on propolis have increased as it has been revealed that contains biologically active molecules. In the current study, was aimed to analyze biological activity, and cytotoxicity ethanolic extract three different samples from Türkiye. Material methods: The antibacterial activity extracts against 14 microorganisms assessed using agar well diffusion method microdilution method. Chromobacter violeceum used in quorum-sensing assay, Pseudomonas aeruginosa PAO1 strain swarming biofilm assays. Using MTT test, cytotoxic effect examined lung adenocarcinoma cell line (A549), pancreatic tumoral (AR42J), breast cancer (MDA-MB-231), normal epithelial (Vero). Main results: All were effective 8/14 included study. While all shown anti-quorum sensing there not any anti-swarming anti-biofilm each sample. It demonstrated every sample had a dose-dependent lines. Research highlights: Due by is considered potential influence creation novel medications future.

Language: Английский

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A Comprehensive review on exploring Thymoquinone as novel therapeutic molecule for clinical management of Glioblastoma Multiforme

Pharmacological Research - Natural Products, Journal Year: 2024, Volume and Issue: unknown, P. 100107 - 100107

Published: Oct. 1, 2024

Language: Английский

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EFFECTS OF EXTRACTION METHODS AND EXTRACTION VARIABLES ON BIOACTIVE COMPOUNDS OF PROPOLIS

GIDA / THE JOURNAL OF FOOD, Journal Year: 2023, Volume and Issue: 48(6), P. 1123 - 1131

Published: Oct. 12, 2023

Propolis içeriğinde bulundurduğu biyoaktif bileşenler sayesinde antik çağlardan beri geleneksel tıpta kullanılan, günümüzde ise zengin içeriği çok sayıda çalışmaya konu olmuş ve olacak bir arı ürünüdür. Doğal antioksidan antimikrobiyal maddelere olan ilgi tüketicinin bilinçlenmesi ile artmaktadır. Bilimin şeyi etkili en verimli yapma çabası bileşenlerin ekstraksiyonunu alan birçok yol açmıştır. Propolisten inceleyen, metotlardan modern tekniklere kadar yöntemin kullanıldığı araştırmalar bu çalışmada incelenmiş olup, yöntemlerin birbirine karşı etkinliği vurgulanmıştır. Farklı çözücü tiplerinin, farklı ekstraksiyon süresi sıcaklıklarının araştırdığı çalışmalar literatürde mevcuttur. Ekstraksiyon işlemine etki eden değişkenin mevcut olması nedeniyle propolis ekstraksiyonunda değişkenlerinin optimizasyonu çalışmalara ihtiyaç duyulmaktadır.

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