Procedure for a standardized preparation of skin prick test solutions for the diagnosis of occupational type I allergies in the absence of commercial extracts

Allergologie select, Journal Year: 2024, Volume and Issue: 8(1), P. 238 - 250

Published: Jan. 1, 2024

In order to ensure valid diagnostics for occupational test allergen solutions despite the ongoing reduction in availability of commercial extracts, a plan B was initiated possible production skin prick (SPT) public pharmacies. For important sources (wheat and rye, storage mites, animal epithelia, mold material) laboratory extraction methods were analyzed comparison pharmacy compatible regarding protein quantity quality SDS-PAGE combined with silver staining. Subsequently, using example bovine adapted procedures as well in-process final product controls transferred pharmacy. Allergen high content, such wheat rye grains showed good comparability extractable pattern, regardless applied method. contrast, source materials low total epithelia molds, can benefit from conditions mechanical disruption specific buffer additives. qualitative staining, characteristic patterns identified each source. Depending on method, only minor differences observed molds. Using two suppliers, resulting extracts displayed clear content mites quantitative A practical preparation attempt SPT successful. prepared comparable Bos d 2 equivalent qualities pattern compared previously available solution. Accordingly, it be assumed that standardized sufficient pharmacies if certified appropriate are available.

Language: Английский

Diagnostic Workup in IgE-Mediated Allergy to Asteraceae Weed Pollen and Herbal Medicine Products in Europe

Medicina, Journal Year: 2024, Volume and Issue: 60(9), P. 1494 - 1494

Published: Sept. 13, 2024

Anemophilous weeds from the Asteraceae family are highly allergenic and represent a significant source of aeroallergens in late summer autumn. Ragweed mugwort pollen allergies have become health burden Europe. Some people with respiratory to weed may also suffer hypersensitivity reactions herbal medicines obtained certain cross-reactive plants Compositae family, such as chamomile, marigold, purple coneflower. General physicians, ear, nose, throat (ENT) specialists, pulmonologists need be familiar diagnostic tests used by allergists clinical practice support accurate diagnosis patients. Allergists must aware suggestions European Medicines Agency (EMA)'s Herbal Medicinal Products Committee broad spectrum therapies educate their patients about potential risks.

Language: Английский