Transcriptional Regulation of Neuropeptide Receptors Decodes Complexity of Peptidergic Modulation of Behavior and Physiology. DOI Creative Commons

SeungHeui Ryu,

Yanan Wei, Zekun Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 23, 2024

ABSTRACT The modulation of complex behaviors in response to environmental and physiological contexts is a fundamental aspect animal biology, with neuropeptides (NPs) playing crucial role this process. This study investigates the transcriptional regulation neuropeptide receptors (NPRs) as mechanism for context-dependent neuropeptidergic physiology behavior. We hypothesize that control NPR genes, rather than NPs themselves, critical determinant behavior physiology. Using multi-faceted approach, including comparative genomics, transcription factor network analysis, empirical validation model organisms such Drosophila melanogaster , we reveal regulatory landscape where expression tightly controlled. Our findings demonstrate genes exhibit higher number enhancers, CTCF-binding sites, open chromatin regions compared NP suggesting greater susceptibility modulation. architecture allows precise over signaling, enabling dynamic context-specific behavioral responses. results highlight importance NPR-expressing cells by mediating effects on show conserved across species, indicating an evolutionarily significant fine-tuning signaling. Furthermore, our provides insights into distinct mechanisms underlying multifunctionality their receptors, offering novel perspective behaviors. In conclusion, advances understanding signaling focusing NPRs. have broad implications development therapeutic strategies targeting systems various neurological disorders.

Language: Английский

Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Open Access
Tanya Wolff, Mark Eddison, Nan Chen

et al.

Published: Jan. 21, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed molecule neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Language: Английский

Citations

2
Cellular and molecular organization of the Drosophila foregut DOI Creative Commons
Haolong Zhu, William B. Ludington, Allan C. Spradling

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(11)

Published: March 5, 2024

The animal foregut is the first tissue to encounter ingested food, bacteria, and viruses. We characterized adult Drosophila using transcriptomics better understand how it triages consumed items for digestion or immune response manages resources. Cell types were assigned validated GFP-tagged Gal4 reporter lines. Foregut-associated neuroendocrine cells play a major integrative role by coordinating gut activity with nutrition, microbiome, circadian cycles; some express clock genes. Multiple epithelial cell comprise proventriculus, central organ that secretes peritrophic matrix (PM) lining gut. Analyzing synthesizing individual PM layers revealed abundant mucin production close enterocytes, similar mammalian intestinal mucosa. esophagus salivary gland secreted proteins likely line esophageal surface, of which may generate commensal niche housing specific microbiome species. Overall, our results imply coordinates dietary sensing, hormonal regulation, immunity in manner has been conserved during evolution.

Language: Английский

Citations

9
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Open Access
Tanya Wolff, Mark Eddison, Nan Chen

et al.

Published: Feb. 28, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Language: Английский

Citations

1
miR-7977 regulates the locomotor behavior by targeting diuretic hormone and SIFamide receptors in Tribolium castaneum DOI Creative Commons

Xiaochuan Pang,

Y. Li,

Roujian Lu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 307, P. 141929 - 141929

Published: March 10, 2025

Insect neuropeptides are crucial for chemical communication, influencing growth, metabolism, and behavior. MicroRNAs (miRNAs), as non-coding RNAs, primarily regulate target gene expression. However, the co-regulation between miRNAs in modulating locomotor behavior remains poorly understood. In this study, we found that miR-7977 inhibited expression of DHR decreased activity adults Tribolium castaneum. Moreover, over-expression lead to a decline both respiratory rate heart rate, an effect not observed upon knockdown, thus prompting our speculation about existence additional genes. Further investigations validated hypothesis. Ultimately, confirmed can SIFR modulate Our research unveils regulatory network miR-7977-DHR/SIFR, offering novel perspectives on intricate regulation insect physiological by small RNAs neuropeptides.

Language: Английский

Citations

1
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Creative Commons
Tanya Wolff, Mark Eddison, Nan Chen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 22, 2024

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent

Language: Английский

Citations

6
Synaptic connectome of a neurosecretory network in the Drosophila brain DOI Creative Commons
Theresa H. McKim, Jayati Gera,

Ariana Julia B. Gayban

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 29, 2024

Abstract Hormones mediate inter-organ signaling which is crucial in orchestrating diverse behaviors and physiological processes including sleep activity, feeding, growth, metabolism reproduction. The pars intercerebralis lateralis insects represent major hubs contain neurosecretory cells (NSC) that produce various hormones. To obtain insight into how hormonal regulated, we have characterized the synaptic connectome of NSC adult Drosophila brain. Identification neurons providing inputs to multiple subtypes implicates diuretic hormone 44-expressing as a coordinator physiology behavior. Surprisingly, despite most having dendrites subesophageal zone (primary taste processing center), gustatory are largely indirect. We also deciphered pathways via olfactory relayed NSC. Further, our analyses revealed substantial from descending NSC, suggesting regulate both endocrine motor output synchronize changes with appropriate behaviors. In contrast inputs, sparse mostly mediated by corazonin Therefore, additionally determine putative paracrine interconnectivity between peripheral tissues analyzing single-cell transcriptomic datasets. Our comprehensive characterization network provides platform understand complex networks they orchestrate animal physiology.

Language: Английский

Citations

5
The neuroendocrine and endocrine systems in insect – Historical perspective and overview DOI
Ian Orchard, Angela B. Lange

Molecular and Cellular Endocrinology, Journal Year: 2023, Volume and Issue: 580, P. 112108 - 112108

Published: Nov. 11, 2023

Language: Английский

Citations

11
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Open Access
Tanya Wolff, Mark Eddison, Nan Chen

et al.

Published: Jan. 21, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Language: Английский

Citations

0
Comparative perspectives on neuropeptide function and social isolation DOI
Kenta Asahina, Moriel Zelikowsky

Biological Psychiatry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Regulation of Feeding and Defecation in Drosophila by Trpγ, Piezo, and DH44R2 DOI

Sonali Puri,

Dharmendra Kumar Nath, Youngseok Lee

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 104267 - 104267

Published: Feb. 1, 2025

Language: Английский

Citations

0