Natural Polyamine Spermidine Inhibits the In Vitro Oxidation of LDL DOI Creative Commons
Christine Rossmann,

Azra Darko,

Gerd Kager

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 955 - 955

Published: Feb. 19, 2025

Spermidine is a natural autophagy-inducer and anti-aging compound. Herein, we investigated potential autophagy-independent mechanism of spermidine, namely its capability to directly impede LDL oxidation, an early step in atherogenesis. In our vitro-model, oxidation was induced by the addition CuCl2 presence increasing concentrations degree lipid, as well protein part LDL, measured. We found that spermidine concentration-dependently inhibited production lipid hydroperoxides, malondialdehyde, oxidation-specific immune epitopes particle, associated with decreased relative electrophoretic mobilities, respectively. For example, LPO content significantly lower when oxidized 500 µg/mL (26.9 ± 1.6 nmol/mg LDL) than absence (180.6 7.7 p < 0.0001). When oxLDL obtained under concentrations, cytotoxicity EA.hy926 cells decreased. Quantum chemical calculations show reaction between hydroxyl radicals exergonic. conclude direct inhibitor due scavenge radicals. Thus, supplementation might be suitable tool atherogenesis (cardio)vascular diseases. Further prospective clinical studies are needed evaluate atheroprotective/health-promoting effects spermidine-rich diets.

Language: Английский

Natural Polyamine Spermidine Inhibits the In Vitro Oxidation of LDL DOI Creative Commons
Christine Rossmann,

Azra Darko,

Gerd Kager

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 955 - 955

Published: Feb. 19, 2025

Spermidine is a natural autophagy-inducer and anti-aging compound. Herein, we investigated potential autophagy-independent mechanism of spermidine, namely its capability to directly impede LDL oxidation, an early step in atherogenesis. In our vitro-model, oxidation was induced by the addition CuCl2 presence increasing concentrations degree lipid, as well protein part LDL, measured. We found that spermidine concentration-dependently inhibited production lipid hydroperoxides, malondialdehyde, oxidation-specific immune epitopes particle, associated with decreased relative electrophoretic mobilities, respectively. For example, LPO content significantly lower when oxidized 500 µg/mL (26.9 ± 1.6 nmol/mg LDL) than absence (180.6 7.7 p < 0.0001). When oxLDL obtained under concentrations, cytotoxicity EA.hy926 cells decreased. Quantum chemical calculations show reaction between hydroxyl radicals exergonic. conclude direct inhibitor due scavenge radicals. Thus, supplementation might be suitable tool atherogenesis (cardio)vascular diseases. Further prospective clinical studies are needed evaluate atheroprotective/health-promoting effects spermidine-rich diets.

Language: Английский

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