Modulation of MMP9 and AKT by Escin in Retinal Pigment Epithelial Cells: Exploring Novel Therapeutic Approaches for Proliferative Vitreoretinopathy

Clinical and Experimental Health Sciences, Journal Year: 2025, Volume and Issue: 15(1), P. 175 - 181

Published: March 23, 2025

Objective: The aim of this study was to investigate the anti-inflammatory and antioxidant effects Escin molecule obtained from horse chestnut seed extract on retinal pigment epithelial cell lines (ARPE-19). Methods: In research, ARPE-19 line, which is a commercially available line derived normal eyes 19-year-old male, utilized. administered cells in varying concentrations 100, 50, 10, 5, 1 micromolar throughout 48-hour timeframe. IC50 concentration subsequently determined through MTT viability assays. To determine migration, wound healing assay executed. quantify MMP9 AKT protein levels, analysis conducted using Western blot. Additionally, mRNA expression levels EGF, EGFR, PDGF-β, PDGFβ-R, HIF1A were analyzed RT-PCR. Results: inhibited migration RPE cells. blot showed that escin decreased proteins. Furthermore, it found PDGFß, suppressed following administration. Conclusion: has potential slow disease progression by suppressing With its anti-angiogenic properties, shows promise for developing new therapeutic approaches treatment diseases.

Language: Английский

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Qualitative Investigation and Screening of Antimicrobial Activity of Stem Extract of Clerodendrum Infortunatum Plant

Journal for Research in Applied Sciences and Biotechnology, Journal Year: 2024, Volume and Issue: 3(3), P. 67 - 75

Published: June 14, 2024

Clerodendrum infortunatum is also known as hill glory bower. plants are widely distributed throughout the whole world. Up to now, many species of C. have been described in various indigenous systems medicine that used preparation folklore medicines for treatment life-threatening disease, and more very well studied their chemical constituents biological activities It Unani, Ayurveda, siddha system years. In Infortunatum compounds, including monoterpentine its derivatives, sesquiterpene, di-terpenoids, tri-terpentine, flavonoid, quercetin, acacetin, gallic acid, sterols flavonoid glycoside, phenylethanoid steroids steroid glycosides, cyclohexylethanoids, anthraqunine, cyanogenic others isolated identified. present study, Chloroform, pet.ether, water stem extract obtained by Soxhlet extraction was screened detect presence or absence several bioactive compounds which reported cure different diseases. Anti-microbial analysis carried out against lacto-bacillus, E.coli staphylococcus aureus organisms agar diffusion method.It observed zone recorded this organism. The results indicates chloroform, pet.ether having anti- microbial efficiency controlling microorganisms. So, clerodendrum plant benificial on human health.

Language: Английский

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A Comparative Study of In Vitro Antibacterial Activity Capacity of Leaf Extract of Zanthoxylum armatum (Timur)

Journal for Research in Applied Sciences and Biotechnology, Journal Year: 2024, Volume and Issue: 3(3), P. 53 - 58

Published: June 14, 2024

Zanthoxylum armatum also known as Timru and toothache tree. This is a sub deciduous aromatic, branched, scandent, or erect shrub small tree that grows to 6–7 meter in height belongs the Rutaceous family. plant common hot valleys of Himalayas from Jammu Bhutan, Nepal, Pakistan. It covers considerable portion Orissa Andhra Pradesh India considered divine contiguous areas. The seeds contain many important phytoconstituents essential oils which contribute its biological activities including larvicidal, anti-inflammatory, analgesic, antinociceptive, antioxidant, antibiotic, hepatoprotective, antiplasmodial, cytotoxic, antiproliferative, anthelmintic, antiviral antifungal. contains alkaloids, oil, resins, sterols, triterpenes, glycosides, saponins, tannins flavonoids. Because Significant medicinal properties excessive increase demand has put this category endangered plants. research paper focuses on Antibacterial activity zanthoxylum plant.

Language: Английский

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Evaluation of the Antioxidant Activity of a Leaf Extract of Cranberry (Vaccinium macrocarpon)

Journal for Research in Applied Sciences and Biotechnology, Journal Year: 2024, Volume and Issue: 3(3), P. 76 - 80

Published: June 14, 2024

Introduction: Cranberry (Vaccinium Macrocarpon) is one of the extremely accessible conventionally used herbal plants with various biological activities. However, actions on antioxidant properties and chemically investigated for its phytoconstituents in current studied. Therefore, scope exploration to screen effects leaf extracts. Materials Methods: The activity (in vitro) was assessed UV Spectroscopic. Hydrogen peroxide (free radical) scavenging methods were employed check vitro property. Result: Plant methanol, Ether Distilled water extract had a substantial impact hydrogen radical less than standard bioactive compound like flavanoids, tannins glycosides shown be positive. Conclusion: These findings suggested that plant leaves are comprised significant properties. It could promising source existence other therapeutic agents.

Language: Английский

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Review on Allopurinol in the Treatment of Gout

Journal for Research in Applied Sciences and Biotechnology, Journal Year: 2024, Volume and Issue: 3(6), P. 68 - 74

Published: Dec. 27, 2024

Gout and pain are synonymous, a study in this issue of the BJP reports novel anti-nociceptive effect allopurinol, drug most commonly used to treat gout. Allopurinol works by inhibiting xanthine oxidase (XO), enzyme responsible for converting hypoxanthine uric acid which is deposited as crystals joints gout sufferers. Hypoxanthine metabolite of, possible precursor to, adenosine. find that acute inhibition XO with allopurinol produces modest adenosine A1 receptor-mediated common tests chemical thermal nociception mice. A concomitant increase cerebrospinal fluid levels supports their hypothesis increases via salvage from hypoxanthine. Elevating endogenous metabolism well-established strategy producing anti-nociception many preclinical models, but likely be particularly beneficial some chronic states because pro-nociceptive reactive oxygen species produced activity. Thus, may have unexpected benefits associated inflammation, diabetes vascular dysfunction.

Language: Английский

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Current Prospective of Hypertension Disease and their Future Aspects in Different Stage of Female Health

Journal for Research in Applied Sciences and Biotechnology, Journal Year: 2024, Volume and Issue: 3(6), P. 60 - 67

Published: Dec. 27, 2024

Cardiovascular disease is the primary cause of mortality in women. Due to age and worsening risk factors over menopausal transition, coronary heart events increases postmenopausal women with diabetes. Randomized studies have conflicted regarding beneficial impact estrogen therapy upon intermediate cardiovascular markers events. Therefore, not currently recommended for indications other than symptom management. However, at low adverse events, can be used minimize symptoms. The estimated using engines calculation breast cancer conjunction screening tools such as mammography. Use therapy, statins, anti-platelet agents guided by calculators particularly younger Risk management remains focused lifestyle behaviors achieving optimal levels factors, including lipids, glucose, blood pressure. pharmacologic therapies address these specific hypoglycemic agents, may provide some additional benefit prevention. minimal limited life expectancy complications intensive should also considered.

Language: Английский

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Diagnostic Tool for Diabetic Retinopathy using Thermal Eye Images

Published: Jan. 1, 2023

Diabetic Retinopathy (DR) occurs as an effect of Diabetes, affecting the retina causing loss vision. Worldwide 27% diabetic individuals are estimated to have DR, leading 0.4 million blindness. As DR population increases every year, early diagnosis is need hour avoid further progression disease thereby preventing In this work, performed using thermal images eye. According earlier research on eye, increase in ocular surface temperature caused by dilatation less with retinopathy than control participants, making it a possible parameter for diagnosis. This imaging modality non-invasive and time consuming easier diagnostic tool large scale screening. For work real acquired from both eyes 20 participants 16 total 62 eye images. The significant features pre-processed extracted Scale invariant transform Gabor Transform methods. processed optimal Machine learning algorithms classify normal accuracy proposed tools 96%. results indicate that IR system could be efficient method non-contact detection anomalies patients diabetes.

Language: Английский

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Effects of Vitex Agnus Castus Leave Extract on Induced Retinal Angiogenesis in Rabbit

Indian Journal of Forensic Medicine & Toxicology, Journal Year: 2021, Volume and Issue: 15(3), P. 1508 - 1514

Published: May 17, 2021

Retinal pathologic angiogenesis is the leading cause of blindness in human generate from age-relatedmacular degeneration (AMD), diabetic retinopathy and premature (ROP).The aim this studyis to investigate effect vitex agnus castus leave extract on induced retinal rabbitsby intravitreal injection VEGF. Alcoholic prepared formulated forintravitreal injection. Twenty four pigmented rabbits included study allocated into equalgroups(6 each group): group, group treated by bevacizumab, grouptreated 100mcg Vitex extract, 50mcg castusleave as Parameters include matrixmetalloproteinase-9, intracellular adhesionmolecule-1. The result current revealed that leaveextract was reduced level MMP-9 ICAM-1 comparison with inducedgroup after one week induction , although it non-significant (P>0.05).

Language: Английский

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