International Journal for Research in Applied Science and Engineering Technology,
Journal Year:
2023,
Volume and Issue:
11(8), P. 1057 - 1069
Published: Aug. 13, 2023
Abstract:
A
simple,
precise,
accurate
method
was
developed
for
the
estimation
of
molnupiravir
by
RP
HPLC.
Chromatographic
conditions
used
are
stationary
phase
Hemochrom
C18
(4.6mm
x
250mm
5μm),
mobile
methanol
5
minutes
then
0.1%
orthophosphoric
acid
and
in
a
ratio
50:50
from
–
20
min.
The
flow
rate
maintained
at
1.0
ml/min,
detection
wavelength
235
nm,
column
temperature
set
to
30.
C
diluent
were
water
(50:50)
finalized
as
an
optimized
method.
System
suitability
parameters
studied
injecting
standard
six
times
results
well
under
acceptance
limits.
Theretention
time
10
validated
linearity,
accuracy,
precision,
specificity,
limit
quantification,
detection,
robustness.
quantificationwas
found
be
0.5
μg/ml
1.5
respectively
recovery
capsule
98.72
%.
proposed
successfully
applied
quantitative
determination
formulation.
Journal of The Electrochemical Society,
Journal Year:
2024,
Volume and Issue:
171(6), P. 067507 - 067507
Published: June 3, 2024
For
the
first
time,
a
green
ecofriendly
approach
is
applied,
to
develop
molecularly
imprinted
polymer
(MIP)
electrochemical
for
assay
of
oral
anti-viral
molnupiravir
(MLN)
in
various
matrices
as
confirmed
by
referring
analytical
eco-scale,
procedure
index
(GAPI),
Raynie
and
Driver,
greenness
metric
(AGREE),
national
environmental
(NEMI).
AuNPs
were
chronoamperometry
deposited
on
glassy
carbon
electrode
surface
(GCE)
using
0.01
gm
ml
−1
gold
solution
(HAuCl
4
.3H
2
O)
0.5
M
H
SO
at
constant
potential
−0.4
V
60
s.
Then,
MIP
was
created
electropolymerizing
O
PDA
GCE
cyclic
voltammetry
range
between
−0.5
+1
15
cycles
(scan
rate:
50
mV.s
).
To
optimize
sensor’s
performance,
factors
such
number
electropolymerization
cycles,
template:
monomer
ratio,
binding
rebinding
pH
buffer,
extraction
solvent,
deposition
time
Au
nanoparticles,
order
electrode;
surface,
well
differential
pulse
parameters
including
accumulation
step,
all
investigated.
The
detection
limit
set
0.00098
ng
(3
×
10
−12
M)
linearity
MLN
0.033
164
(1
−10
–5
−7
M).
sensor
employed
determination
its
pharmaceutical
product
(capsules),
spiked
human
plasma,
urine
samples
with
mean
recovery
%
±
relative
standard
deviation
(RSD)
(99.82%
0.53),
(99.88%
0.62),
(97.90%
0.70),
respectively.
investigated
provided
good
reproducibility,
repeatability,
durability.
Acceptable
selectivity
regarding
when
mixed
structurally
comparable
compounds
proved
RSD
(97.10%
0.03).
Additionally,
exposed
oxidative,
hydrolytic,
thermal
stress
conditions,
results
stability-indicating
studies
served
an
indicator
selectivity.
developed
coupled
portable
potentiostat,
making
it
promising
point-of-care
diagnostic
platform
on-site
measurements.
International Journal of Applied Pharmaceutics,
Journal Year:
2025,
Volume and Issue:
unknown, P. 204 - 224
Published: March 7, 2025
Objective:
In
the
current
study,
a
new
approach
was
developed
for
simultaneous
determination
of
molnupiravir
with
its
degradation
products
using
RP-HPLC
by
following
Analytical
Quality
Design
(AQbD)
approach,
proactive
strategy
advancing
pharmaceutical
research.
Methods:
Development
carried
out
applying
two
levels
fractional
factorial
design
(2[5-1]
Resolution:
V,
four
center
points,
and
no
replicates)
to
five
independent
factors.
The
separation
achieved
liquid
chromatography,
equipped
XBridge
BEH
Shield
(250
mm
x
4.6
mm,
3.5
µm)
RP18
column
at
260
nm
gradient
elution.
A
force
study
performed
in
different
stressed
conditions
major
degradant
identified
modern
analytical
techniques.
Results:
dependent
factors
were
assessed
against
H-N
plot,
Pareto
chart,
3D
surface
plot
applied
through
ANOVA.
Major
obtained
from
stress
characterized
LC-MS,
NMR,
FTIR
method
found
be
specific,
precise
(%
RSD
≤
2.0),
linear
(r
=
0.9999)
accurate
(99.2-101.4
%)
0.2-0.6
mg/ml
range.
LOD
LOQ
0.02
%
(0.08
µg/ml,
S/N
ratio
≥
3)
0.05
(0.2
10,
RSD:
2.2)
respectively.
solutions
stable
48
hour
clear
glassware
room
temperature.
depicts
successful
development
validation
assay
related
substances
Experiments
(DoE)
approach.
Conclusion:
Fractional
Factorial
(FFD)
experiment
explored
interactions
Expert
software,
version
13.0.
This
AQbD-driven
provided
an
in-depth
understanding
chromatographic
factors,
ensuring
reliability
effectiveness
simultaneously
determining
purity
impurity
molnupiravir,
assisting
regulatory
compliance.
Research Journal of Pharmacy and Technology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1680 - 1687
Published: April 12, 2025
Molnupiravir
is
an
anti-viral
medication.
A
new
stability
indicating
RP-UFLC
method
was
proposed
for
the
estimation
of
in
capsules.
Shimadzu
Model
CBM-20A/20
Alite
UFLC
system
with
PDA
detector
and
Zorbox
C18
column
were
used
chromatographic
study.
Mobile
phase
mixture
consisting
0.1%
Acetic
acid:
Acetonitrile
(35:65,
v/v)
a
flow
rate
0.8
ml/min
chosen
elution
(Detection
wavelength
245
nm).
The
linear
over
concentration
range
2.0-100
mg/ml
regression
equation,
y
=
29137x
+
3455.8
(R²
0.9999).
LOD
LOQ
found
to
be
0.4574
1.548
respectively.
Forced
degradation
studies
performed
validated
as
per
ICH
guidelines.
RSC Advances,
Journal Year:
2023,
Volume and Issue:
13(19), P. 13224 - 13239
Published: Jan. 1, 2023
Since
the
discovery
of
first
case
infected
with
severe
acute
respiratory
syndrome
coronavirus-2
(SARS
CoV-2)
in
Wuhan,
China
December
2019,
it
has
turned
into
a
global
pandemic.
According
to
World
Health
Organization
(WHO)
statistics,
about
603.7
million
confirmed
coronavirus
cases
and
6.4
deaths
have
been
reported.
Remdesivir
(RMD)
was
U.S.
Food
Drug
Administration
(FDA)
approved
antiviral
drug
for
treatment
pediatrics
adults
different
disease
severities,
ranging
from
mild
severe,
both
hospitalized
non-hospitalized
patients.
Various
regimens
are
used
Covid-19
treatment,
all
which
rely
on
use
agents
including
ritonavir
(RTN)/nirmatrelvir
(NTV)
combination,
molnupiravir
(MLP)
favipiravir
(FVP).
Optimizing
analytical
methods
selective
sensitive
quantification
above-mentioned
drugs
pharmaceutical
dosage
forms
biological
matrices
is
must
current
Several
techniques
were
reported
estimation
antivirals
therapy.
Chromatographic
include
Thin
Layer
Chromatography
(TLC)
densitometry,
High
Performance
(HPTLC),
Reversed
Phase-High
Liquid
(RP-HPLC),
Tandem
Mass
Spectrometry
(HPLC-MS/MS)
or
Ultraviolet
detectors
(HPLC-UV),
Ultra
High-Performance
(UHPLC-MS/MS)
(UPLC-UV)
Micellar
(MLC).
In
addition
other
spectroscopic
Paper
Spray
(PS-MS),
UV-Visible
Spectrophotometry,
Spectrofluorimetry.
Herein,
we
will
focus
clarification
trendy,
simple,
rapid,
accurate,
precise,
sensitive,
selective,
eco-friendly
analysis
anti-Covid-19
as
well
matrices.
Research Journal of Pharmacy and Technology,
Journal Year:
2024,
Volume and Issue:
unknown, P. 5210 - 5214
Published: Nov. 18, 2024
A
type
of
antiviral
medicine
called
molnupiravir
is
used
to
treat
viral
infections.
Using
green
solvent
as
methanol,
a
precise
and
affordable
UV
spectrophotometric
technique
has
been
created.
It
was
demonstrated
how
determine
the
Molnupiravir
concentration
in
pharmaceutical
dose
formulations
bulk
samples
at
preset
λ
max
236nm.
Beer
limitations
were
found
have
regression
equation
(y=0.0457x-0.0031)
an
excellent
correlation
coefficient
(R2=0.999).
The
quantity
marketed
brands
from
local
markets
determined
using
this
method
successfully,
results
agreement
with
claim
on
label.
According
standards
International
Conference
Harmonisation
(ICH),
method's
linearity,
accuracy,
precision,
robustness,
ruggedness
all
validated.
obtained
outcomes
suitability
for
regular
analysis
well
formulation.
BMC Chemistry,
Journal Year:
2023,
Volume and Issue:
17(1)
Published: Sept. 21, 2023
Abstract
COVID-19
is
a
serious
virus
that
can
have
lot
of
effects,
one
which
secondary
bacterial
infection
be
more
life-threatening
and
even
lethal
than
the
initial
viral
infection.
Hence
fast
sensitive
HPLC/UV
method
was
developed
validated
for
first
estimation
binary
mixture
molnupiravir
(MOL)
ertapenem
(ERT)
as
co-administrated
medicine
management
in
pharmaceutical
dosage
forms,
human
plasma
samples.
The
drug
combination
separated
within
5
min
via
RP-ODS
column
using
isocratic
elution
with
mobile
phase
0.05
M
phosphate
buffer
(pH
3.5):
acetonitrile
76:
24%
ratio
v/v.
presented
provided
linear
response
ranging
from
0.03
to
17.0
0.05–20
µg
mL
−1
LOD
values
0.009
0.008
MOL
ERT
respectively.
good
separation
high
sensitivity
HPLC
provide
determination
cited
drugs
without
matrix
interference
percent
recovery
94.97
±
2.05
98.44
1.92.
Based
on
results,
this
could
utilized
monitor
quality
control
therapeutic
laboratories.
Delta University Scientific Journal,
Journal Year:
2024,
Volume and Issue:
7(1), P. 0 - 0
Published: April 1, 2024
The
outbreak
of
the
COVID-19
pandemic
has
resulted
in
a
significant
global
mortality
rate.
According
to
World
Health
Organization
(WHO),
virus
impacted
population
exceeding
500
million
people,
leading
around
6.5
deaths.
antiviral
medications
remdesivir
and
molnupiravir
are
pre-existing
drugs
that
were
strategically
repositioned
repurposed
context
combating
pandemic.
determination
these
antivirals
required
establishment
rapid,
sensitive,
precise,
reliable
methodologies.
current
review
entails
thorough
examination
various
analytical
methods
have
been
reported
for
quantitative
estimation
drugs,
this
includes
spectrophotometric,
spectrofluorometric,
electrochemical,
chromatographic
techniques.
Those
techniques
applied
quantify
examined
single
dosage,
co-formulated
synthetic
mixture
as
well
biological
fluids.
Therefore,
aim
is
highlight
assay
both
drugs.
