Exploitation of waste biomass of the invasive alga species Rugulopteryx okamurae in Morocco: In-vitro antibacterial-antibiofilm properties and GC- MS metabolite profiling
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 8, 2025
Abstract
Since
2017,
the
invasive
brown
alga
Rugulopteryx
okamurae
has
posed
significant
biological,
ecological,
and
socio-economic
challenges
in
Morocco
due
to
its
rapid
proliferation
accumulation.
This
study
explores
antimicrobial
antibiofilm
properties
chemical
composition.
Methanol,
chloroform,
ethyl
acetate
(EtOAc)
extracts
were
evaluated
for
antibacterial
activity
against
six
human
pathogens,
including
clinical
reference
strains
of
Escherichia
coli
Staphylococcus
aureus.
Fourier-transform
infrared
spectroscopy
(FT-IR)
identified
functional
groups
such
as
hydroxyl
(O-H),
carbonyl
(C
=
O),
polysaccharides,
indicative
bioactive
compounds.
Gas
chromatography-mass
spectrometry
(GC-MS)
revealed
key
components:
oleic
acid
butyl
ester
EtOAc
extract,
methyl
tetradecanoate
chloroform
pentadecanoic
methanolic
extract.
The
potential
was
assessed
using
microdilution
growth
curve
assays,
with
minimal
inhibitory
bactericidal
concentrations
ranging
from
3.12–50
mg/mL.
Growth
analysis
showed
bacteriostatic
effects
through
lag
phase
extension
rate
suppression.
extract
demonstrated
highest
activity,
reducing
biofilm
formation
by
73%
E.
(clinical
strain
TT-23)
70%
S.
aureus
70).
These
results
suggest
that
R.
contains
compounds
promising
properties,
potentially
valuable
mitigating
impacts
while
offering
applications
development.
Language: Английский
Sulphated polysaccharide extracted from the Mediterranean edible seaweed Caulerpa racemosa: structural, rheological and biological features
Sustainable Chemistry and Pharmacy,
Journal Year:
2025,
Volume and Issue:
45, P. 102034 - 102034
Published: April 24, 2025
Language: Английский