Acta Chromatographica,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Nov. 29, 2023
Abstract
In
this
study,
two
different
ethanol-based
RP-HPLC
methods
for
assay
and
quantification
of
rivaroxaban
related
substances
in
tablets
were
developed,
based
on
green
analytical
chemistry
(GAC)
principles,
using
the
design
experiments
approach.
The
chromatographic
separation
was
performed
X-Bridge
C18
column
(250
×
4.6
mm,
5
µm
particle
size),
isocratic
elution
with
ethanol
:
water
(35:65,
%
v/v
)
gradient
ethanol/water
mobile
phase,
substances,
a
flow
rate
1.0
mL
min
−1
.
method
optimized
three
specified
impurities
(impurity
G,
impurity
H,
14)
selectivity
further
confirmed
forced
degradation
studies.
Both
validated
accordance
ICH
guidelines.
robustness
Central
Composite
Face
Design
Experiments.
Analytical
Eco-scale
approach
AGREE
metrics
that
both
are
GAC
principles.
proposed
applied
determination
10
mg
obtained
from
manufacturers
available
Macedonian
market.
IntechOpen eBooks,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Feb. 11, 2023
Increasing
interest
in
topics
related
to
health
and
quality
of
life
recent
years
has
led
a
growing
need
food,
environmental
bioanalytical
research
for
high-throughput
separation
techniques
able
cope
with
the
qualitative/quantitative
determination
large
number
compounds
very
complex
matrices.
High-performance
liquid
chromatography
(HPLC)
is
well-established
technique
widely
employed
many
fields.
The
versatility
chromatographic
modes,
coupled
low-resolution
high-resolution
mass
spectrometry,
makes
HPLC
among
best
options
solve
emerging
analytical
problems.
This
book
provides
an
overview
new
advances
high-performance
its
applications
different
Natural Products and Bioprospecting,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Jan. 2, 2025
The
integration
of
phytochemistry
into
forensic
science
has
emerged
as
a
groundbreaking
frontier,
providing
unprecedented
insights
nature's
secrets
through
the
precise
application
phytochemical
fingerprinting
phytotoxins
cutting-edge
approach.
This
study
explores
dynamic
intersection
and
science,
highlighting
how
unique
profiles
toxic
plants
their
secondary
metabolites,
serve
distinctive
markers
for
investigations.
By
utilizing
advanced
techniques
such
Ultra-High-Performance
Liquid
Chromatography
(UHPLC)
High-Resolution
Mass
Spectrometry
(HRMS),
detection
quantification
plant-derived
are
made
more
accurate
in
contexts.
Real-world
case
studies
presented
to
demonstrate
critical
role
plant
toxins
outcomes
legal
proceedings.
challenges,
potential,
future
prospects
integrating
were
discussed.
review
aims
illuminate
promising
tool
enhance
precision
depth
analyses,
offering
new
complex
stories
embedded
toxins.
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(46), P. 20444 - 20456
Published: Nov. 7, 2024
Humic
substances
are
organic
mixtures
of
extreme
complexity,
which
significantly
complicate
their
analysis
by
any
method.
Fractionation
into
more
homogeneous
seems
to
be
almost
the
only
way
overcome
these
difficulties.
Preparative
high-performance
liquid
chromatography
(HPLC)
provides
amounts
required
for
both
further
fractionation
and
studies
other
methods.
For
first
time,
isopropyl
alcohol
(IPA)
is
proposed
purposes;
its
advantages
shown
example
groundwater
fulvic
acids
(FA).
IPA
much
safer
than
conventional
solvents
elutes
most
nonpolar
compounds
that
nonelutable
methanol
acetonitrile.
The
isolated
fractions
differ
in
molecular
composition,
confirmed
ultrahigh-resolution
mass
spectrometry
spectroscopy.
Stepwise
IPA-gradient
HPLC-UV
each
preparative
fraction
demonstrates
possibilities
multidimensional
HPLC
FA.
were
studied
contents
a
broad
range
elements,
relationships
between
trace-element
compositions
revealed.
Our
data
reveals
existence
numerous
different
organometal
FA
composition;
thus,
approach
can
used
in-depth
study
mechanisms
element
migration
environment.
