Heliyon,
Journal Year:
2024,
Volume and Issue:
11(1), P. e41588 - e41588
Published: Dec. 31, 2024
Detection
of
biomolecules,
Glutathione
(GSH)
in
particular,
is
important
because
it
helps
assess
antioxidant
capacity,
cellular
protection,
detoxification
processes,
and
potential
disease
associations.
Monitoring
glutathione
levels
can
provide
valuable
information
about
overall
health
well-being.
Many
medical
disorders
have
been
connected
to
levels.
Higher
seen
several
cancer
cell
types,
which
may
increase
their
resistance
radiation
chemotherapy.
be
measured
through
various
methods,
such
as
colorimetric
assays
fluorescent
probes.
However,
surface-enhanced
Raman
scattering
(SERS)
has
known
an
efficient
selective
technique
for
biomolecule
detection.
Here
this
perspective
review,
we
reported
two
distinctive
methods
based
on
SERS
detection
GSH;
heat-induced
method
reversed
reporting
agent
method.
Several
variables
that
impact
the
scheme
were
elaborated
"heat-induced
method,"
including
pretreatment,
nanoparticle
reduction
time,
process
temperature,
pH
colloidal
solution,
concentration
citrate
buffer,
participating
nanoparticles.
To
choose
best
a
reverse
using
approaches,
agents
examined
second
In
order
grasp
situation
at
hand,
detection-specifically,
GSH
schemes-was
briefly
discussed.
spectroscopy
its
associated
terminology
then
covered
followed
by
outlook
end.
meet
demands
real-time
applications
everyday
life
enhance
detection-in
detection-such
thorough
investigation
unavoidable.
Nutrients,
Journal Year:
2021,
Volume and Issue:
13(8), P. 2791 - 2791
Published: Aug. 14, 2021
The
prevalence
of
gastritis
in
humans
is
constantly
growing
and
a
prediction
an
increase
this
health
problem
observed
many
countries.
For
reason,
effective
dietary
therapies
are
sought
that
can
alleviate
the
course
disease.
objective
study
was
to
determine
effect
chemically
pure
oat
beta-glucan
preparations
with
different
molar
masses,
low
or
high,
used
for
30
days
patients
histologically
diagnosed
chronic
gastritis.
enrolled
48
people
both
genders
ages
recruited
from
129
diagnosis.
Before
after
therapy,
hematological,
biochemical,
immunological
redox
balance
parameters
were
determined
blood
number
lactic
acid
bacteria
SCFA
concentrations
feces.
Our
results
demonstrated
beneficial
beta-glucans
high
mass
humans,
resulting
reduced
mucosal
damage
healthy
changes
fecal
concentration
peripheral
serum
glutathione
metabolism
antioxidant
defense
parameters.
This
fraction
highly
purified
safe
humans.
Its
action
use,
which
sheds
new
light
on
nutritional
treatment
PeerJ,
Journal Year:
2024,
Volume and Issue:
12, P. e18382 - e18382
Published: Nov. 11, 2024
Background
Oxidative
stress
caused
by
exposure
to
ultraviolet
(UV)
light
on
the
skin
can
damage
deoxyribonucleic
acid
(DNA)
and
cause
keratinocytes
undergo
apoptosis.
Endogenous
antioxidants
which
play
a
role
in
trapping
free
radicals
are
also
unable
overcome
excess
reactive
oxygen
species
(ROS)
body
due
UV
exposure,
so
exogenous
needed.
Polyphenolic
compounds
extracted
from
natural
ingredients
such
as
flavonoids,
quercetin,
epicatechin
have
quite
strong
antioxidant
activity.
This
is
influenced
chemical
structure
of
these
rich
hydroxyl
groups
aromatic
groups.
allows
compound
become
an
electron
donor
that
it
neutralize
radicals.
In
vitro
research
was
used
see
potential
effectiveness
antiaging
antioxidant.
The
study
aims
confirm
assay.
Methods
viability
test
human
fibroblast
(BJ)
cells
carried
out
using
water-soluble
tetrazolium
(WST)
BJ
were
UV-induced
cell
model
premature
aging.
Epicatechin
6.25,
12.5,
25
µg/mL
administered
cells.
gene
expression
Collagen
I
Alpha
1
(COL1A1),
matrix
metalloproteinase-1
(MMP-1),
growth
factor-2
(FGF-2),
glutathione
peroxidase-1
(GPX-1)
analyzed
quantitative
real-time
polymerase
chain
reaction
(qRT-PCR).
Elastin
(ELN),
hyaluronidase
(HAase),
cyclooxigenase-2
(COX-2),
8-hydroxydeoxyguanosine
(8-OhdG),
melatonin
(MT)
protein
levels
enzyme-linked
immunosorbent
assay
(ELISA).
apoptosis
flow
cytometry.
Results
Treatment
with
increased
relative
including
COL1A1
(5.94),
FGF-2
(8.34),
GPX-1
(8.09),
decreased
MMP-1
(2.90)
compared
ELN
(107.7
ng/mg
protein)
MT
(830
treatment
HAase
(505.96
protein),
COX-2
(33.69
8-OHdG
(97.87
succeeded
maintaining
percentage
live
reducing
apoptosis,
necrotic
Conclusions
has
be
agent
Cells,
Journal Year:
2021,
Volume and Issue:
10(9), P. 2419 - 2419
Published: Sept. 14, 2021
Cryptosporidiosis
is
caused
by
an
opportunistic
protozoan
parasite
(Cryptosporidium
parvum
and
C.
hominis)
known
as
a
of
humans,
especially
children
immunocompromised
patients.
The
current
study
was
designed
to
evaluate
the
therapeutic
efficacy
mixture
fig
olive
leaf
extracts
alternative
medicinal
plant.
Parasitological
examination
for
oocysts
in
stool
histopathological
alterations
small
intestines
were
examined.
Additionally,
biochemical
analyses
liver
kidney
functions
addition
antioxidant
parameters
such
superoxide
dismutase
(SOD),
glutathione
peroxidase
(GSH)
catalase
(CAT)
plasma
evaluated.
Our
results
showed
that
marked
reduction
shedding
amelioration
intestinal
changes
hepatic
or
renal
detected
all
treated
groups
compared
control
infected
group.
with
tested
at
ratios
1:3
1:5
significant
decrease
number
other
groups.
Results
exhibited
increase
SOD,
CAT
GSH
levels
one.
This
suggested
convenient
promising
agent
Cryptosporidiosis.
Nutrients,
Journal Year:
2023,
Volume and Issue:
15(3), P. 656 - 656
Published: Jan. 28, 2023
Obesity,
and
its
consequences
for
human
health,
is
a
huge
complicated
problem
that
has
no
simple
solution.
The
constant
search
natural
safe
compounds
with
systemic
action
can
be
used
obesity
prophylactics
treatment
hampered
by
the
limited
availability
variable
quality
of
biomass
wild
medicinal
plants.
Plant
cell
biotechnology
an
alternative
approach
sustainable
production
vegetative
or
individual
phytochemicals
high
therapeutic
potential.
In
this
study,
suspension
plants,
Dioscorea
deltoidea
Wall.,
Tribulus
terrestris
L.,
Panax
japonicus
(T.
Nees)
C.A.
Mey,
produced
in
20
L
630
bioreactors,
were
tested
effects
rat
models
alimentary-induced
obesity.
Three-month
intake
water
infusions
dry
(100
mg/g
body
weight)
against
background
hypercaloric
diet
reduced
weight
gain
proportion
fat
mass
obese
animals.
addition,
preparation
intracellular
dehydration
balanced
amounts
intra-
extracellular
fluids
as
determined
bioimpedance
spectroscopy.
A
significant
decrease
glucose
cholesterol
levels
blood
was
also
observed
result
administration
all
species.
Hypocholesterolemic
activity
line
P.
>
D.
T.
terrestris/liraglutide
intact
group
control
group.
By
sum
parameters
tested,
culture
considered
most
effective
mitigating
diet-induced
obesity,
positive
sometimes
exceeding
those
reference
drug
liraglutide.
safety
assessment
phytopreparation
showed
toxic
effect
on
reproductive
function
animals
their
offspring.
These
results
support
potential
application
biotechnologically
plant
species
remedies
related
complications,
particularly
long-term
during
pregnancy
lactation
periods
when
conventional
often
contraindicated.
Journal of Chemical Education,
Journal Year:
2023,
Volume and Issue:
100(7), P. 2591 - 2600
Published: June 26, 2023
Molecules
and
Computer:
Chemistry
Calculations
in
Class
(MC4)
is
a
computational
laboratory
intended
for
final-year
high
school
or
undergraduate
students.
The
topic
the
antioxidant
potential
of
anthocyanidins,
which
chemically
related
to
their
radical
scavenging
action
via
mechanism
hydrogen
atom
transfer
(HAT).
This
combines
(bio)chemical
nutraceutical
concepts
with
organic
chemical
reactions
involving
species.
It
allows
students
apply
important
physicochemical
(thermodynamic)
concepts,
such
as
Gibbs
free
energy
reaction
solvation.
Finally,
procedure
can
easily
be
tailored
resources
at
hand
well
knowledge
In
fact,
when
computing
facilities
are
not
available,
whole
set
molecular
structures
data
provided
simple
datasheet
required
analysis.
Alternatively,
protocol
useful
scripts
so
that
generate
own
results
by
experiencing
approach
chemistry.
Journal of Personalized Medicine,
Journal Year:
2024,
Volume and Issue:
14(3), P. 221 - 221
Published: Feb. 20, 2024
Background:
Multiple
myeloma
is
a
hematological
malignancy
characterized
by
anemia,
antibodies
causing
kidney
damage,
and
damage
to
multiple
organs,
which
come
together
cause
morbidity.
Although
oxidative
stress
not
core
pathological
aspect
of
myeloma,
reactive
oxygen
species
(ROS)
antioxidant
balance
have
been
shown
play
role
in
the
disease
process
are
considered
its
management.
In
presented
study,
we
aim
assess
reliability
specific
oxidant
variables
as
potential
biomarkers
for
determine
these
might
exhibit
higher
sensitivity
predicting
myeloma.
Methods:
This
case-control
study
was
conducted
between
March
2023
August
2023.
A
total
30
patients,
newly
diagnosed
according
diagnostic
criteria
revised
International
Myeloma
Study
Group
2014,
volunteers
without
were
included
this
study.
Serum
glutathione
peroxidase
(GSH-Px),
superoxide
dismutase
(SOD)
catalase
(CAT),
malondialdehyde
(MDA)
nitric
oxide
(NO)
levels
measured
with
first
blood
samples
taken
after
inclusion.
Results:
The
groups
had
similar
age
(p
=
0.623)
sex
distribution
1.000).
MDA
(cut-off:
>4.35,
p
<
0.001),
GSH-Px
(<59.8,
CAT
(<67.2,
SOD
(<21.2,
NO
(>38.5,
0.001)
could
significantly
detect
Multivariate
logistic
regression
revealed
that
increased
0.003)
decreased
independently
associated
disease.
Conclusions:
presence
patients
clearest
indicator
stress.
These
parameters
may
help
identify
therapeutic
targets
develop
strategies
control
progression.
