Age-trajectories of higher-order diffusion properties of major brain metabolites in cerebral and cerebellar grey matter using in vivo diffusion-weighted MR spectroscopy at 3T DOI Creative Commons
Kadir Şimşek, Cécile Gallea, Guglielmo Genovese

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 5, 2024

Abstract Healthy brain aging involves changes in both structure and function, including alterations cellular composition microstructure across regions. Unlike diffusion-weighted MRI (dMRI), MR spectroscopy (dMRS) can assess cell-type specific microstructural changes, providing indirect information on cell through the quantification interpretation of metabolites’ diffusion properties. This work investigates age-related higher-order properties three major intracellular metabolites (N-Acetyl-aspartate, Creatine Choline) beyond classical apparent coefficient cerebral cerebellar grey matter healthy human brain. Twenty-five subjects were recruited scanned using a semi-LASER sequence two regions-of-interest (ROI) at 3T: posterior-cingulate (PCC) cortices. Metabolites’ was characterized by quantifying metrics from Gaussian non-Gaussian signal representations biophysical models. All studied exhibited lower diffusivities higher kurtosis values cerebellum compared to PCC, likely stemming complexity cerebellum. Multivariate regression analysis (accounting for ROI tissue as covariate) showed slight decrease (or no change) all increase with age, none which statistically significant (p>0.05). The proposed age-trajectories provide benchmarks identifying anomalies could be related pathological mechanisms altering composition.

Language: Английский

Assessing potential correlation between T2 relaxation and diffusion of lactate in the mouse brain DOI Creative Commons
Eloïse Mougel,

Sophie Malaquin,

Julien Valette

et al.

Magnetic Resonance in Medicine, Journal Year: 2022, Volume and Issue: 88(5), P. 2277 - 2284

Published: July 30, 2022

While diffusion and T2 relaxation are intertwined, little or no correlation exists between of intracellular metabolites in the rodent brain, as measured by diffusion-weighted MRS at different TEs. However, situation might be for lactate, since it is present both extracellular spaces, which exhibit properties may also . Such a TE dependence would crucial to account when interpreting modeling lactate diffusion. Here we propose take advantage new sequence, where J-modulation canceled even long TE, thus retaining excellent signal, assess potential on mouse brain.Using frequency-selective spin-echo sequence that removes 1.3 ppm, preserving signal investigate effect 50.9 110.9 ms (while keeping time constant) apparent diffusivity kurtosis brain.Regardless metabolites, difference appears attenuation with increasing TE. For remain unchanged increases.No significant 50-110 range, confirming effects can ignored

Language: Английский

Citations

6
Age-trajectories of higher-order diffusion properties of major brain metabolites in cerebral and cerebellar grey matter using in vivo diffusion-weighted MR spectroscopy at 3T DOI Creative Commons
Kadir Şimşek, Cécile Gallea, Guglielmo Genovese

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 5, 2024

Abstract Healthy brain aging involves changes in both structure and function, including alterations cellular composition microstructure across regions. Unlike diffusion-weighted MRI (dMRI), MR spectroscopy (dMRS) can assess cell-type specific microstructural changes, providing indirect information on cell through the quantification interpretation of metabolites’ diffusion properties. This work investigates age-related higher-order properties three major intracellular metabolites (N-Acetyl-aspartate, Creatine Choline) beyond classical apparent coefficient cerebral cerebellar grey matter healthy human brain. Twenty-five subjects were recruited scanned using a semi-LASER sequence two regions-of-interest (ROI) at 3T: posterior-cingulate (PCC) cortices. Metabolites’ was characterized by quantifying metrics from Gaussian non-Gaussian signal representations biophysical models. All studied exhibited lower diffusivities higher kurtosis values cerebellum compared to PCC, likely stemming complexity cerebellum. Multivariate regression analysis (accounting for ROI tissue as covariate) showed slight decrease (or no change) all increase with age, none which statistically significant (p>0.05). The proposed age-trajectories provide benchmarks identifying anomalies could be related pathological mechanisms altering composition.

Language: Английский

Citations

0