ANXA5: related mechanisms of osteogenesis and additional biological functions

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: April 24, 2025

Annexin A5 (ANXA5), also known as V, is a calcium-dependent phospholipid-binding protein and has high affinity with phosphatidylserine (PS). This characteristic facilitates its involvement in wide range of biological functions, including vesicle transport, the formation mineral phases extracellular matrix, anticoagulation antithrombotic, inhibition tumor growth, apoptosis regulation. ANXA5 plays role anti-inflammatory antithrombotic properties. It protective effects on nervous system. been reported to facilitate osteogenic differentiation take part chondrocyte mineralization. More more attention paid potential for bone defect repair. Most current studies mainly concentrate immune disorders, pregnancy disorders serve biomarker various diseases well detection. However, there still lack systematic involving multiple tissues, bone, cartilage, vessels, nerves process regeneration. Our study aims summarize functions tissue related signaling pathways ANXA5. work provides theoretical foundation applying clinical orthopedics future.

Language: Английский

“Dictionary of immune responses” reveals the critical role of monocytes and the core target IRF7 in intervertebral disc degeneration

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 17, 2024

Intervertebral disc degeneration (IDD) is widely regarded as the primary contributor to low back pain(LBP). As an immune-privileged organ, upon onset of IDD, various components nucleus pulposus (NP) are exposed host's immune system, accumulating cytokines. Cytokines facilitate intercellular communication within induce cells polarisation, and exacerbate oxidative stress in IDD.

Language: Английский

Serine protease inhibitor E2 protects against cartilage tissue destruction and inflammation in osteoarthritis by targeting NF-κB signaling

Lara D. Veeken, Journal Year: 2024, Volume and Issue: 63(11), P. 3172 - 3183

Published: Aug. 24, 2024

Abstract Objective OA is a chronic disease characterized by cartilage degeneration and inflammation, with no approved disease-modifying drugs. This study aimed to identify pathogenic genes elucidate their mechanism in OA. Methods We systematically identified combined sing-cell bulk transcriptome profiles of tissues Adenovirus carrying the serpin peptidase inhibitor clade E member 2 (serpinE2) or exogenous serpinE2 was injected into monosodium iodoacetate (MIA)-induced OA-model rats. Histological analysis, immunohistochemistry Alcian blue staining were performed. In vitro, immunofluorescence, quantitative real-time PCR (RT-qPCR), ELISA western blot assays Results exhibited elevated expression hypomethylation, showing positive association collagen pathway activities patients Silencing aggravated MIA-induced knee Conversely, intra-articular injection ameliorated articular degeneration, reduced pain-related behavioural responses relieve synovitis Exogenous not only attenuated elevation NLRP3, IL-1β caspase1 levels but also restored reduction cell viability induced lipopolysaccharide (LPS) chondrocytes. Mechanistically, we found that inhibited LPS-induced reactive oxygen species (ROS) release NF-κB signalling activation. Conclusions plays protective role synovium tissues, suggesting gene transfer molecules upregulate could be therapeutic candidates for

Language: Английский