Research Square (Research Square),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Dec. 5, 2022
Abstract
Background:
Metabolic
reprogramming
caused
by
SHMT1/2,
as
key
enzymes
in
one-carbon
metabolism,
has
been
considered
to
play
a
critical
role
occurrence
and
progression
of
hepatocellular
carcinoma
(HCC).
However,
the
associations
between
single
nucleotide
polymorphisms
(SNPs)
SHMT1/2
genes
susceptibility
HCC
remains
unknown.
The
purpose
this
study
was
investigate
relation
four
candidate
SNPs
risk
Chinese
population.
Materials
methods:
A
case-control
including
514
patients
234
controls
performed
determine
risk,isolated
via
mass
spectrometry.
Results:
Our
data
revealed
that
SHMT2
rs2229717
significantly
associated
with
when
compared
healthy
under
multiple
genetic
models
(Allele
T:
odds
ratio
(OR)
=
0.73,
P=0.023;
Dominant
Model:
OR
0.72,
P=0.044).
When
our
cohort
stratified
sex,
male
subjects
T
allele
TT
genotype
for
exhibited
decreased
(OR=0.67,
95%CI=0.50-0.91;
OR=0.43,
95%CI=0.18-0.99).
In
addition,
after
age
stratification
executed,
results
showed
both
GT
were
less
common
50
years
old.
Conclusion:
These
indicate
rs2229717is
predisposing
factor
may
affect
various
or
sex
subgroups,
suggesting
rs2229717may
be
useful
predictive
diagnostic
tool
some
populations.
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 16, 2023
Abstract
Transcription
factor
3
(TCF3)
has
a
vital
role
in
tumor
occurrence
and
progression.
However,
the
specific
functions
underlying
mechanisms
of
dysregulated
TCF3
hepatocellular
carcinoma
(HCC)
is
not
thoroughly
characterized.
Thus,
we
explored
levels
roles
HCC
samples.
In
addition,
knockdown
overexpression
models
were
developed
via
lentiviral
vectors
cells.
Transwell
as
well
vivo
metastasis
experiments
performed
to
measure
effects
on
cell
metastasis.
Then,
reverse
transcription-quantitative
PCR,
serial
deletion,
western
blotting,
site-directed
mutagenesis,
chromatin
immunoprecipitation
dual-luciferase
reporter
assays
done
determine
involved
pathomechanisms.
markedly
elevated
samples,
correlated
with
poor
prognosis.
Besides,
overexpressed
promoted
invasion
migration,
while
repressed
growth.
mediated
MMP11
expressions.
TCF3-associated
migration
its
attenuated
knockdown-mediated
repression
human-derived
positively
expression
level.
Overall,
these
findings
highlighted
that
could
be
regarded
prognostic
biomarker
regulator.
Research Square (Research Square),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Dec. 5, 2022
Abstract
Background:
Metabolic
reprogramming
caused
by
SHMT1/2,
as
key
enzymes
in
one-carbon
metabolism,
has
been
considered
to
play
a
critical
role
occurrence
and
progression
of
hepatocellular
carcinoma
(HCC).
However,
the
associations
between
single
nucleotide
polymorphisms
(SNPs)
SHMT1/2
genes
susceptibility
HCC
remains
unknown.
The
purpose
this
study
was
investigate
relation
four
candidate
SNPs
risk
Chinese
population.
Materials
methods:
A
case-control
including
514
patients
234
controls
performed
determine
risk,isolated
via
mass
spectrometry.
Results:
Our
data
revealed
that
SHMT2
rs2229717
significantly
associated
with
when
compared
healthy
under
multiple
genetic
models
(Allele
T:
odds
ratio
(OR)
=
0.73,
P=0.023;
Dominant
Model:
OR
0.72,
P=0.044).
When
our
cohort
stratified
sex,
male
subjects
T
allele
TT
genotype
for
exhibited
decreased
(OR=0.67,
95%CI=0.50-0.91;
OR=0.43,
95%CI=0.18-0.99).
In
addition,
after
age
stratification
executed,
results
showed
both
GT
were
less
common
50
years
old.
Conclusion:
These
indicate
rs2229717is
predisposing
factor
may
affect
various
or
sex
subgroups,
suggesting
rs2229717may
be
useful
predictive
diagnostic
tool
some
populations.
