This
thesis
investigates
blood-brain
barrier
(BBB)
transporter
changes
in
Alzheimer’s
disease
(AD)
using
stem
cell
models.
It
reviews
BBB
function
AD
and
successfully
differentiates
cells
into
components.
Findings
suggest
dysregulation
of
certain
transporters
AD,
potentially
contributing
to
onset.
study
also
explores
inflammation's
impact
on
expression
suggests
non-invasive
modulation
as
a
therapeutic
avenue.
Additionally,
it
inflammatory
responses
SARS-CoV-2
spike
protein
exposure
cell-derived
cells,
indicating
potential
implications
for
COVID-19
manifestation.
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: March 24, 2023
Abstract
Glioblastoma
(GBM)
is
the
most
malignant
brain
tumor
and
associated
with
a
low
survival
high
recurrence
rate.
Deletions
mutations
in
phosphatase
tensin
homolog
(PTEN),
suppressor
gene,
are
therapeutic
resistance.
In
this
study,
we
constructed
recombinant
Newcastle
disease
virus
(rNDV)
overexpressing
human
PTEN
(rNDV-PTEN)
GBM
cells.
overexpression
decreased
cell
proliferation
migration
induced
apoptosis
U87
MG
tumorigenesis
was
also
examined
vivo
using
orthotopic
mouse
models.
rNDV
rNDV-PTEN
crossed
blood–brain
barrier
to
reach
brain.
A
reduction
size
mice
treated
intravenously
confirmed
by
magnetic
resonance
imaging.
increased
mTOR
dephosphorylation
autophagy.
As
result,
levels
of
pre-apoptotic
markers
such
as
caspases
3,
8,
9
Bax
were
PTEN-overexpressing
Taken
together,
these
results
suggest
that
via
treatment
promotes
cells
disrupting
signaling
autophagy,
suggesting
new
strategy
treat
GBM.
Current Alzheimer Research,
Journal Year:
2023,
Volume and Issue:
20(6), P. 394 - 402
Published: June 1, 2023
Abstract:
Neurodegenerative
diseases
(NDDs)
encapsulate
conditions
in
which
neural
cell
populations
are
perpetually
degraded
and
nervous
system
function
destroyed.
Generally
linked
to
increased
age,
the
proportion
of
patients
diagnosed
with
a
NDD
is
growing
as
human
life
expectancies
rise.
Traditional
therapies
surgical
interventions
have
been
limited.
However,
recent
breakthroughs
understanding
disease
pathophysiology,
improved
drug
delivery
systems,
targeted
pharmacologic
agents
allowed
innovative
treatment
approaches
treat
NDDs.
A
common
denominator
for
administering
these
new
options
requirement
neurosurgical
skills.
In
present
narrative
review,
we
highlight
exciting
novel
preclinical
clinical
discoveries
being
integrated
into
care.
We
also
discuss
traditional
role
neurosurgery
managing
neurodegenerative
emphasize
critical
effectuating
newly
developed
treatments.
Biochimica et Biophysica Acta (BBA) - General Subjects,
Journal Year:
2023,
Volume and Issue:
1867(12), P. 130481 - 130481
Published: Oct. 5, 2023
Ultrasound-responsive
microbubbles
offer
a
means
of
achieving
minimally
invasive,
localised
drug
delivery
in
applications
including
regenerative
medicine.
To
facilitate
their
use,
however,
it
is
important
to
determine
any
cytotoxic
effects
they
or
constituents
may
have.
The
aim
this
study
was
test
the
hypothesis
that
phospholipid-shelled
are
non-toxic
human
bone-derived
cells
at
biologically-relevant
concentrations.Microbubbles
were
fabricated
using
combinations
1,2-distearoyl-sn-glycero-3-phosphocholine
(DSPC),
1,2-dibehenoyl-sn-glycero-3-phosphocholine
(DBPC),
polyoxyethylene(40)
stearate
(PEG40S)
and
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene-glycol)-2000]
(DSPE-PEG2000).
Microbubble
size
concentration
measured
as
function
time
temperature
by
optical
microscopy.
Effects
on
MG63
osteosarcoma
bone
marrow
stromal
(BMSCs)
for
up
72
h
assay
viability,
metabolic
activity
proliferation.DBPC:DSPE-PEG2000
significantly
more
stable
than
DSPC:PEG40S
under
all
conditions
tested.
Serum-containing
medium
had
no
detrimental
effect
microbubble
stability,
but
storage
37
°C
compared
4
reduced
stability
both
preparations,
with
almost
complete
dissolution
times
≥24
h.
greater
inhibitory
cell
metabolism
growth
DBPC:DSPE-PEG2000
microbubbles,
PEG40S
found
be
principle
component.
These
only
evident
high
concentrations
(≥20%
(v/v))
prolonged
culture
(≥24
h).
Increasing
cell-microbubble
contact
inversion
custom-built
device
metabolism.These
data
indicate
that,
over
broad
range
incubation
times,
have
little
osteoblastic
viability
growth,
component
formulations
investigated.
Frontiers in Neurology,
Journal Year:
2023,
Volume and Issue:
14
Published: Nov. 1, 2023
Due
to
the
complex
pathological
mechanisms
of
Alzheimer's
disease
(AD),
its
treatment
remains
a
challenge.
One
major
difficulties
in
treating
AD
is
difficulty
for
drugs
cross
blood–brain
barrier
(BBB).
Low-intensity
ultrasound
(LIUS)
novel
type
with
neuromodulation
function.
It
has
been
widely
reported
that
LIUS
combined
intravenous
injection
microbubbles
(MB)
can
effectively,
safely,
and
reversibly
open
BBB
achieve
non-invasive
targeted
drug
delivery.
However,
many
studies
have
MB-mediated
opening
(LIUS
+
MB-BBBO)
improve
deposition
cognitive
impairment
patients
mice
without
delivering
additional
drugs.
This
article
reviews
relevant
research
on
MB-BBBO
AD,
analyzes
potential
mechanisms,
summarizes
parameters.
This
thesis
investigates
blood-brain
barrier
(BBB)
transporter
changes
in
Alzheimer’s
disease
(AD)
using
stem
cell
models.
It
reviews
BBB
function
AD
and
successfully
differentiates
cells
into
components.
Findings
suggest
dysregulation
of
certain
transporters
AD,
potentially
contributing
to
onset.
study
also
explores
inflammation's
impact
on
expression
suggests
non-invasive
modulation
as
a
therapeutic
avenue.
Additionally,
it
inflammatory
responses
SARS-CoV-2
spike
protein
exposure
cell-derived
cells,
indicating
potential
implications
for
COVID-19
manifestation.
