Transcriptome Analysis Reveals Sertoli Cells Adapting Through Redox and Metabolic Pathways Under Heat Stress in Goats DOI Open Access
Yang Guang, Yiwei Wang,

Pengyun Ji

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(12), P. 1582 - 1582

Published: Dec. 9, 2024

Background/Objectives: Climate change-induced temperature elevations pose significant challenges to livestock reproduction, particularly affecting testicular function in small ruminants. This study investigates the acute heat-stress response goat Sertoli cells (SCs), aiming elucidate molecular mechanisms underlying heat-induced damage male reproductive tissues. Methods: SCs were isolated from testes of 4-month-old black goats and exposed heat stress (44 °C for 2.5 h). We employed transcriptome sequencing, CCK-8 assay, electron microscopy, ROS measurement, autophagy detection, Western blot analysis, lactate concentration measurement. Bioinformatics analyses including Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway, protein–protein interaction network performed on data. Results: Heat significantly reduced SC viability, induced oxidative autophagy, altered gene expression profiles. identified 1231 differentially expressed genes, with enrichment membrane-related processes metabolic pathways. Metabolism-related PKLR, ACOT11, LPCT12, downregulated. Protein–protein analysis revealed ten hub genes potentially crucial response: HSP90AA1, HSPA5, BAG3, IGF1, HSPH1, IL1A, CCL2, CXCL10, ALB, CALML4. Conclusions: provides comprehensive insights into stress. The pathways, those related metabolism response, offer potential targets developing strategies mitigate effects reproduction. These findings contribute our understanding climate change impacts animal husbandry may inform development resistant lines.

Language: Английский

Low-Intensity Pulsed Ultrasound Responsive Scaffold Promotes Intramembranous and Endochondral Ossification via Ultrasonic, Thermal, and Electrical Stimulation DOI

Wanru Jia,

Tianlong Wang, Feng Chen

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: 19(4), P. 4422 - 4439

Published: Jan. 21, 2025

Multiple physical stimuli are expected to produce a synergistic effect promote bone tissue regeneration. Low-intensity pulsed ultrasound (LIPUS) has been clinically used in repair for the mechanical stimulation that it provides. In addition, LIPUS can also excite biomaterials generate other such as thermal or electrical stimuli. this study, scaffold based on decellularized adipose (DAT) is established by incorporating polydopamine-modified multilayer black phosphorus nanosheets (pDA-mBP@DAT). Their under and potential mechanisms further investigated. This possesses piezoelectric properties generates mild thermogenic stimulus when stimulated LIPUS. With superior properties, demonstrated have good cytocompatibility vitro vivo. Simultaneously, promotes cell attachment, migration, osteogenic differentiation pDA-mBP@DAT scaffold. Furthermore, combined use of significantly affects regenerative rat models critical-sized calvarial defects. The possible include promoting osteogenesis neovascularization activating Piezo1. study presents insight into speeding up regeneration combination scaffolds.

Language: Английский

Citations

1
Ultrasound-assisted immunotherapy for malignant tumour DOI Creative Commons
Xiaowen Cai, Yujie Liu,

Guosheng Luo

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 13, 2025

Malignant tumour represents a significant global public health concern. The advent of immunotherapy has brought about revolutionary shift in the landscape treatment, offering ray hope to patients across globe. Immunotherapy strategies have demonstrated considerable promise clinical trials. However, immunosuppressive environment within microenvironment constituted obstacle advancement immunotherapies. It is therefore imperative develop more efficacious and personalised approaches. utilisation non-invasive ultrasound-assisted promising strategy. Ultrasound capacity induce an immune response stimulate other drugs achieve specific response, thereby reducing toxic side effects treatment enhancing outcome immunotherapy. This paper presents systematic introduction various mechanisms related ultrasound reviews recent advancements immunotherapy, including ultrasonic ablation, combined application with contrast agents, sonodynamic therapy.

Language: Английский

Citations

0
PU-H71 (NSC 750424): a molecular masterpiece that targets HSP90 in cancer and beyond DOI Creative Commons
Sameh Saber, Rasha Abdelhady, Mai A. Elhemely

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 5, 2024

Heat shock protein 90 (HSP90) is a pivotal molecular chaperone with multifaceted roles in cellular health and disease. Herein, we explore how HSP90 orchestrates stress responses, particularly through its partnership heat factor 1 (HSF-1). PU-H71, selective inhibitor of HSP90, demonstrates significant potential cancer therapy by targeting wide array oncogenic pathways. By inducing the degradation multiple client proteins, PU-H71 disrupts critical signaling pathways such as MAPK, PI3K/Akt, JAK/STAT, EGFR, mTOR, which are essential for cell survival, proliferation, metastasis. We examined impact on combating triple-negative breast enhancing effectiveness carbon-ion beam therapy, offering new avenues treatment. Furthermore, dual inhibition HSP90A HSP90B1 proves highly effective context myeloma, providing fresh hope patients this challenging malignancy. delve into to induce apoptosis B-cell lymphomas that rely Bcl6 highlighting relevance realm hematologic cancers. Shifting our focus hepatocellular carcinoma, innovative approaches chemotherapy. Moreover, current review elucidates capacity suppress glial activation paving way developing novel therapeutic strategies neuroinflammatory disorders. Additionally, present report also suggests promising role JAK2-dependent myeloproliferative neoplasms. Eventually, sheds more light functions well benefit an diseases, laying foundations development could achieve better treatment outcomes.

Language: Английский

Citations

1
Anti-inflammatory role of low-intensity pulsed ultrasound in inhibiting lipopolysaccharide-induced M1 polarization of RAW264.7 cells via Wnt2b/AXIN/β-catenin DOI Creative Commons

Juan Juan Yin,

Yu Bao, Minghan Xu

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e18448 - e18448

Published: Nov. 13, 2024

Background Low-intensity pulsed ultrasound (LIPUS) is a special type of low-intensity ultrasound. In periodontal disease, LIPUS applied as an adjuvant and non-invasive treatment. It has been reported that significantly shifts the macrophage phenotype from M1 to M2, but specific mechanism behind this shift still unknown. Methods RAW264.7 cells were induced M1/M2 polarization with lipopolysaccharide (LPS)/interleukin-4 (IL4). was performed for 25 min two times, 24 h apart, at intensity 45 mW/cm 2 stimulate cells. PolyA mRNA sequencing conducted both LPS-induced The expression Wnt2b in downregulated by siRNA. surface markers downstream inflammatory cytokines detected using flow cytometry. relative proteins Wnt2b/AXIN/β-catenin pathway assessed reverse transcription real-time polymerase chain reaction (RT-qPCR) Western blot. Results reversed cells, decreased CD80 CD86. addition, enhanced M2 upregulated CD163 CD206. polyA results indicated Wnt signaling participated LIPUS-treated RAW264.7. RT-qPCR showed higher M1- or M2-polarized Knocking down shown inhibitory effect on increase knockdown also regulated AXIN, β-catenin, factors such tumor necrosis factor alpha (TNFα) interleukin-6 (IL6). Conclusions plays anti-inflammatory role inhibiting Wnt2b/AXIN/β-catenin-dependent way. may play therapeutic diseases inflammation through regulation differentiation.

Language: Английский

Citations

0
Transcriptome Analysis Reveals Sertoli Cells Adapting Through Redox and Metabolic Pathways Under Heat Stress in Goats DOI Open Access
Yang Guang, Yiwei Wang,

Pengyun Ji

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(12), P. 1582 - 1582

Published: Dec. 9, 2024

Background/Objectives: Climate change-induced temperature elevations pose significant challenges to livestock reproduction, particularly affecting testicular function in small ruminants. This study investigates the acute heat-stress response goat Sertoli cells (SCs), aiming elucidate molecular mechanisms underlying heat-induced damage male reproductive tissues. Methods: SCs were isolated from testes of 4-month-old black goats and exposed heat stress (44 °C for 2.5 h). We employed transcriptome sequencing, CCK-8 assay, electron microscopy, ROS measurement, autophagy detection, Western blot analysis, lactate concentration measurement. Bioinformatics analyses including Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway, protein–protein interaction network performed on data. Results: Heat significantly reduced SC viability, induced oxidative autophagy, altered gene expression profiles. identified 1231 differentially expressed genes, with enrichment membrane-related processes metabolic pathways. Metabolism-related PKLR, ACOT11, LPCT12, downregulated. Protein–protein analysis revealed ten hub genes potentially crucial response: HSP90AA1, HSPA5, BAG3, IGF1, HSPH1, IL1A, CCL2, CXCL10, ALB, CALML4. Conclusions: provides comprehensive insights into stress. The pathways, those related metabolism response, offer potential targets developing strategies mitigate effects reproduction. These findings contribute our understanding climate change impacts animal husbandry may inform development resistant lines.

Language: Английский

Citations

0